Integrated transcriptome and metabolome analysis revealed hub genes and metabolites associated with subzero temperature tolerance following cold acclimation in rapeseed (Brassica rapa L.).

Rapeseed naturally endures low temperature in late autumn and early winter to develop cold adaptation, named as cold acclimation (CA). The underlying mechanism by which CA induces plant resistance to subzero temperature tolerance is unclear. This study examined the transcriptome and metabolome of strong (Longyou 7) and weak (Longyou 99) cold-tolerant rapeseed varieties treated with treatments CA and sub-zero temperatures at fourth leaf stage.

Identification of ARF gene family and functional analysis of CqARF05 under drought and salt stress in quinoa.

The ARF gene family is crucial for regulating plant growth, development, and responses to various signaling pathways. In this study, 26 quinoa ARF genes (CqARF01-CqARF26) were identified, with encoded proteins varying in length from 553 to 1092 amino acids, molecular weights from 60.66 to 111.

Novel insights into the unique characterization of N6-methyladenosine RNA modification and regulating cold tolerance in winter Brassica rapa.

N6-methyladenosine (mA) is an mRNA modification considered essential in plants, and is a key player in gene regulation at the transcriptional and translational levels. In present study, we mapped Brassica rapa's whole transcriptome mA profile under low-temperature stress in different cold tolerant varieties to elucidate the mA methylation pattern. The distribution of mA modifications changed significantly under low temperature stress.

Integrated transcriptome and metabolome analysis revealed molecular regulatory mechanism of saline-alkali stress tolerance and identified bHLH142 in winter rapeseed (Brassica rapa).

Soil salinization is one of the main problems leading to a reduction in arable land area. In the present study, strongly salt-tolerant lines were screened for germination rates and physiological indices. The mechanism of saline-alkali stress tolerance in winter rapeseed was examined using transcriptome and metabolome analyses.

Methyl-Sensitive Amplification Polymorphism (MSAP) Analysis Provides Insights into the DNA Methylation Changes Underlying Adaptation to Low Temperature of L.

Background: DNA methylation can change rapidly to regulate the expression of stress-responsive genes. Previous studies have shown that there are significant differences in the cold resistance of winter rapeseed ( L.) after being domesticated in different selection environments; however, little is known about the epigenetic regulatory mechanisms of its cold resistance formation.

Impact of Straw Incorporation on the Physicochemical Profile and Fungal Ecology of Saline-Alkaline Soil.

Improving the soil structure and fertility of saline-alkali land is a major issue in establishing a sustainable agro-ecosystem. To explore the potential of different straw returning in improving saline-alkaline land, we utilized native saline-alkaline soil (SCK), wheat straw-returned saline-alkaline soil (SXM) and rapeseed straw-returned saline-alkaline soil (SYC) as our research objects. Soil physicochemical properties, fungal community structure and diversity of saline-alkaline soils were investigated in different treatments at 0-10 cm, 10-20 cm and 20-30 cm soil depths.

Genome-wide identification of RNA recognition motif (RRM1) in Brassica rapa and functional analysis of RNA-binding protein (BrRBP) under low-temperature stress.

Background: The RNA recognition motif (RRM) is primarily engaged in the processing of mRNA and rRNA following gene transcription as well as the regulation of RNA transport; it is critical in preserving RNA stability.

Results: In this study, we identified 102 members of the RRM1 gene family in Brassica rapa, which were dispersed across 10 chromosomes with the ninth chromosome being the most extensively distributed. The RRM1 gene family members of Brassica rapa and Arabidopsis thaliana were grouped into 14 subclades (I-XIV) using phylogenetic analysis.

Cloning and functional analysis of the gene in L.

The gene plays an important role in the formation of apical meristem and organ edges in plants. The apical meristematic tissue of () is associated with cold resistance, however, the role of the gene in cold resistance of is unclear. In this study, we used bioinformatics software to analyze the structure of gene, real-time fluorescence quantitative PCR to detect the expression level of , constructed transgenic by the flower dipping method and subcellular localization for functional validation.

Physiological Analysis and Genetic Mapping of Short Hypocotyl Trait in L.

Hypocotyl length is a botanical trait that affects the cold tolerance of L. (). In this study, we constructed an F segregating population using the cold-resistant short hypocotyl variety '16VHNTS158' and the cold-sensitive long hypocotyl variety 'Tianyou 2288' as the parents, and BSA-seq was employed to identify candidate genes for hypocotyl length in .

Genome-Wide Identification of Gene Family and Functional Analysis of of Winter Rapeseed ( L.) under Cold Stress.

The largest gene families in plants were found to be Glutathione transferases (GSTs), which played significant roles in regulating plant growth, development, and stress response. Within the gene family, members were found to play a crucial role in the low-temperature response process of plants. A comprehensive study identified a total of 70 genes.

Identification of CDK gene family and functional analysis of CqCDK15 under drought and salt stress in quinoa.

as one of the oldest cultivated crops in the world, quinoa has been widely valued for its rich nutritional value and green health. In this study, 22 CDK genes (CqCDK01-CqCDK22) were identified from quinoa genome using bioinformatics method. The number of amino acids was 173-811, the molecular weight was 19,554.

Genome-Wide Identification of C2H2 ZFPs and Functional Analysis of under Low-Temperature Stress in Winter Rapeseed ().

Zinc-finger protein (ZFP) transcription factors are among the largest families of transcription factors in plants. They participate in various biological processes such as apoptosis, autophagy, and stemness maintenance and play important roles in regulating plant growth and development and the response to stress. To elucidate the functions of ZFP genes in the low-temperature response of winter ( L.

A Chromosome Level Genome Assembly of a Winter Turnip Rape (.) to Explore the Genetic Basis of Cold Tolerance.

Winter rapeseed (.) is an important overwintering oilseed crop that is widely planted in northwest China and suffers chronic low temperatures in winter. So the cold stress becomes one of the major constraints that limit its production.

iTRAQ-based quantitative proteome analysis insights into cold stress of Winter Rapeseed (Brassica rapa L.) grown in the field.

Winter rapeseed (Brassica rapa L.) is a major oilseed crop in Northern China, where its production was severely affected by chilling and freezing stress. However, not much is known about the role of differentially accumulated proteins (DAPs) during the chilling and freezing stress.

Salicylic acid induces tolerance of Vitisriparia×V.labrusca to chilling stress by altered photosynthetic, antioxidant mechanisms and expression of cold stress responsive genes.

The yield and quality of wine grapes are severely persecuted by low-temperaturestresses. Salicylic acid (SA) assists plants in coping with abiotic stresses such as drought, heavy metal toxicity, and osmotic stress. The objective of this study was to evaluate the effect of foliar spraying of different concentrations of SA on the mitigation of cold damage in grapes, which is useful for the cultivation of wine grapes.

Number variation of high stability regions is correlated with gene functions.

Various regulatory elements in messenger RNAs (mRNAs) carrying the secondary structure play important roles in a wide range of expression processes. Numerous recent works have focused on the discovery of these functional elements that contain the conserved mRNA structures. However, to date, regions with high structural stability have been largely overlooked.

Universally increased mRNA stability downstream of the translation initiation site in eukaryotes and prokaryotes.

Local secondary structures in coding sequences have important functions across various translational processes. To date, however, the local structures and their functions in the early stage of translation elongation remain poorly understood. Here, we surveyed the structural stability in the first 180 nucleotides of the coding sequence of 27 species using computational method.