Transient glycan-shield reduction induces CD4-binding site broadly neutralizing antibodies in SHIV-infected macaques.

Unlabelled: Broadly neutralizing antibodies (bNAbs) targeting the HIV-1 CD4-binding site (CD4bs) occur infrequently in macaques and humans and have not been reproducibly elicited in any outbred animal model. To address this challenge, we first isolated RHA10, an infection-induced rhesus bNAb with 51% breadth. The cryo-EM structure of RHA10 with HIV-1 envelope (Env) resembled prototypic human CD4bs bNAbs with CDR-H3-dominated binding.

Interventions to improve patient health education competence among nursing personnel: A scoping review.

Aim: To determine what intervention strategies have been used in interventions aimed at improving the patient health education competence of nursing personnel.

Background: There is a growing body of research on educational interventions for improving nursing personnel's patient health education competence, but there are significant differences in the teaching objectives, content and methods of these studies, as well as a lack of standardized educational strategies.

Design: The scoping review was based on the Joanna Briggs Institute methodology.

Integrating Chemoselective Labeling and Laser-Cleavable Mass Tagging for Determination of Sialic Acids in Glycoconjugates.

Sialic acids are the terminal units of glycans in glycoproteins or glycolipids. The determination of sialic acids in glycoconjugates is crucial since they regulate essential biological functions and have a significant nutritional value. To achieve a specific and high-throughput in situ determination of sialic acids in glycoconjugates, a laser-desorption/ionization mass spectrometry (LDI-MS)-based strategy is reported by integrating chemoselective labeling and laser-cleavable mass tagging.

Interventions to enhance the core competencies of clinical nursing preceptors: a protocol for a systematic review.

Introduction: Clinical nursing preceptors (CNPs) teach nursing skills to students in real medical scenarios and develop their professionalism. The adequacy of CNPs' teaching competencies affects the effectiveness of student learning, so it is crucial to seek the best evidence for teaching competency interventions. This report describes a protocol for a systematic review to identify and analyse interventions to enhance the teaching competencies of CNPs.

Flexible silicon for high-performance photovoltaics, photodetectors and bio-interfaced electronics.

Silicon (Si) is currently the most mature and reliable semiconductor material in the industry, playing a pivotal role in the development of modern microelectronics, renewable energy, and bio-electronic technologies. In recent years, widespread research attention has been devoted to the development of advanced flexible electronics, photovoltaics, and bio-interfaced sensors/detectors, boosting their emerging applications in distributed energy sources, healthcare, environmental monitoring, and brain-computer interfaces (BCIs). Despite the rigid and brittle nature of Si, a series of new fabrication technologies and integration strategies have been developed to enable a wide range of c-Si-based high-performance flexible photovoltaics and electronics, which were previously only achievable with intrinsically soft organic and polymer semiconductors.

Interventions to enhance the core competencies of clinical nursing preceptors: A systematic review.

Unlabelled: Aim This systematic review aimed to identify intervention studies on core competencies of clinical nursing preceptors, evaluate their quality using quantitative indicators, and summarize the effectiveness of the intervention programs and limitations of included studies.

Design: This review was reported in accordance with the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) 2020 checklist.

Data Sources: Between May 1, 2024 and May 30, 2024, we searched PubMed, Web of Science, CINAHL, Medline, EMBASE and ProQuest (Health & Medical Collection) to get relevant literature published between January 2010 and May 2024.

Interventions to improve patient health education competence among nursing personnel: a scoping review protocol.

Introduction: Patient health education has gradually become an indispensable and important part of nursing work. However, nursing personnel's performance in this domain remains below satisfactory levels. The absence of patient health education competence (PHEC) constitutes a significant impediment to the effective implementation of such education by nursing personnel.

Aberrant METTL1-mediated tRNA mG modification alters B-cell responses in systemic autoimmunity in humans and mice.

Upon activation, naive B cells exit their quiescent state and enter germinal center (GC) responses, a transition accompanied by increased protein synthesis. How protein translation efficiency is adequately adjusted to meet the increased demand requires further investigation. Here, we identify the methyltransferase METTL1 as a translational checkpoint during GC responses.

Classification and epidemiologic analysis of 86 diseases in .

Following the release of in May 2018, the Chinese government officially published in September 2023. To date, there is no unified standard and international consensus for rare diseases, and epidemiologic data for most rare diseases in China are lacking. We investigated 86 rare diseases on the second list using Orphanet and other databases to clarify the classification, nomenclature, and epidemiologic data for these diseases, and we summarized the genotype and phenotype of hereditary diseases.

The Application of Augmented Reality Technology in Perioperative Visual Guidance: Technological Advances and Innovation Challenges.

In contemporary medical practice, perioperative visual guidance technology has become a critical element in enhancing the precision and safety of surgical procedures. This study provides a comprehensive review of the advancements in the application of Augmented Reality (AR) technology for perioperative visual guidance. This review begins with a retrospective look at the evolution of AR technology, including its initial applications in neurosurgery.

Serum level of IFN-λ is elevated in idiopathic inflammatory myopathies.

Background: Idiopathic inflammatory myopathies (IIM) are a heterogeneous group of autoimmune disorders with uncertain pathogenesis. Interferon (IFN)-λ has recently been described as an important mediator of immune responses. The main purpose of this study is to find out whether IFN-λ is involved in IIM.

Role of Rab35 in modulating lipid metabolism and viral entry during pseudorabies virus infection.

Pseudorabies virus (PRV), the causative agent of Aujeszky's disease in swine, is a significant pathogen in veterinary medicine. Rab35 is a key regulatory GTPase involved in diverse cellular functions, including endocytic recycling, cytokinesis, and the regulation of the actin cytoskeleton. Although Rab35's roles in these processes are well-documented, its contribution to PRV replication dynamics had not been previously elucidated.

The vitamin D receptor is essential for the replication of pseudorabies virus.

The vitamin D receptor (VDR) is a nuclear steroid receptor that regulates the expression of genes across various biological functions. However, the role of VDR in pseudorabies virus (PRV) infection has not yet been explored. We discovered that VDR positively influenced PRV proliferation because knockdown of VDR impaired PRV proliferation, whereas its overexpression promoted it.

Potent and broad HIV-1 neutralization in fusion peptide-primed SHIV-infected macaques.

An antibody-based HIV-1 vaccine will require the induction of potent cross-reactive HIV-1-neutralizing responses. To demonstrate feasibility toward this goal, we combined vaccination targeting the fusion-peptide site of vulnerability with infection by simian-human immunodeficiency virus (SHIV). In four macaques with vaccine-induced neutralizing responses, SHIV infection boosted plasma neutralization to 45%-77% breadth (geometric mean 50% inhibitory dilution [ID] ∼100) on a 208-strain panel.

SLM Magnesium Alloy Micro-Arc Oxidation Coating.

In this study, we utilized Selective Laser Melting (SLM) technology to fabricate a magnesium alloy, and subsequently subject it to micro-arc oxidation treatment. We analyzed and compared the microstructure, elemental distribution, wetting angle, and corrosion resistance of the SLM magnesium alloy both before and after the micro-arc oxidation process. The findings indicate that the SLM magnesium alloy exhibits surface porosity defects ranging from 2% to 3.

Ablation of mitophagy receptor FUNDC1 accentuates septic cardiomyopathy through ACSL4-dependent regulation of ferroptosis and mitochondrial integrity.

Sepsis evokes compromised myocardial function prompting heart failure albeit target therapy remains dismal. Our study examined the possible role of mitophagy receptor FUNDC1 in septic cardiomyopathy. A sepsis model was established using cecal ligation and puncture (CLP) in FUNDC1 knockout (FUNDC1) and WT mice prior to the evaluation of cardiac morphology, echocardiographic and cardiomyocyte contractile, oxidative stress, apoptosis, necroptosis, and ferroptosis.

Methylation-related differentially expressed genes as potential prognostic biomarkers for cervical cancer.

Aim: To discover novel methylation-related differentially expressed genes (MRDEGs) for cervical cancer, with a focus on their potential for early diagnosis and prognostic assessment.

Materials & Methods: We integrated data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. TCGA-MRDEGs were identified by analyzing differentially methylated genes (DMGs) and their correlation with gene expression.

Decoding the origins, spread, and global risks of mcr-9 gene.

Background: The global spread of the plasmid-mediated mcr (mobilized colistin resistance) gene family presents a significant threat to the efficacy of colistin, a last-line defense against numerous Gram-negative pathogens. The mcr-9 is the second most prevalent variant after mcr-1.

Methods: A dataset of 698 mcr-9-positive isolates from 44 countries is compiled.

Causal Association of Golgi Protein 73 With Coronary Artery Disease: Evidence from Proteomics and Mendelian Randomization.

Identification of the unknown pathogenic factor driving atherosclerosis not only enhances the development of disease biomarkers but also facilitates the discovery of new therapeutic targets, thus contributing to the improved management of coronary artery disease (CAD). We aimed to identify causative protein biomarkers in CAD etiology based on proteomics and 2-sample Mendelian randomization (MR) design. Serum samples from 33 first-onset CAD patients and 31 non-CAD controls were collected and detected using protein array.

PathoTracker: an online analytical metagenomic platform for Klebsiella pneumoniae feature identification and outbreak alerting.

Clinical metagenomics (CMg) Nanopore sequencing can facilitate infectious disease diagnosis. In China, sub-lineages ST11-KL64 and ST11-KL47 Carbapenem-resistant Klebsiella pneumoniae (CRKP) are widely prevalent. We propose PathoTracker, a specially compiled database and arranged method for strain feature identification in CMg samples and CRKP traceability.

Layer-by-layer fabrication of covalent organic frameworks on stainless steel needles as solid-phase microextraction probe coupled with electrospray ionization mass spectrometry for enrichment and determination of tyrosine kinase inhibitors in biosamples.

Sunitinib, N-desmethyl imatinib, dasatinib, imatinib, and bosutinib are tyrosine kinase inhibitors (TKIs) that are commonly employed in the treatment of a multitude of cancers. However, the inappropriate concentrations of TKIs can result in ineffective treatment or the emergence of multiple adverse effects. Consequently, the development of a rapid and sensitive analytical method for TKIs is of paramount importance for the safe administration of drugs.

Pectin-based cinnamon essential oil Pickering emulsion film with two-sided differential wettability: A major role in the spatial distribution of microdroplets.

Pickering emulsions have attracted much attention as a novel emulsifying technology. This research to explore Zein-Citrus pectin nanoparticles stabilized cinnamon essential oil (CEO) Pickering emulsion (ZCCPEs) for constructing Pickering emulsion edible film (PEF). Unlike traditional research, which focuses on antibacterial and antioxidant activities, our research examined the physical properties of PEF, specifically changes in wettability.

A homogeneous binary matrix assisted laser desorption/ionization time-of-flight mass spectrometry assay for determination of artificial sweeteners in beverages.

Artificial sweeteners have been widely used as additives in various beverages. Due to the safety risks associated with artificial sweeteners, it is essential to develop a simple, rapid, and high-throughput method for the analysis of artificial sweeteners. Here, we report a homogeneous binary matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) assay for the simultaneous analysis of sweeteners including aspartame (ASP), neotame (NEO), and advantame (ADV) with a simple dilution step.