Molecular mechanism of protein-lipid interactions in steamed egg gelation and deterioration: A quantitative proteomic study.

Steamed egg (SE), a traditional egg dish, exhibits steaming time-dependent textural properties. This study investigated the molecular mechanisms underlying SE gel formation and deterioration through quantitative proteomics combined with physicochemical characterization. Results showed optimal gel formation at 11 min steaming, while prolonged steaming (23 min) led to gel cracking and sensory deterioration.

Research note: Proteomics profiling reveal key proteins in egg white emulsification.

Egg white proteins are widely recognized as excellent natural emulsifiers, yet the molecular mechanisms underlying their emulsification properties remain incompletely understood, particularly regarding the roles of individual proteins in complex natural systems. Using 4D-label-free quantitative proteomics, we systematically investigated protein dynamics during egg white emulsification by comparing egg white (EW) and the aqueous phases of egg white emulsions (EWE-W). Proteomic analysis identified 96 distinct proteins, with 64 showing significant abundance changes during emulsification.

Circulating TERT serves as the novel diagnostic and prognostic biomarker for the resectable NSCLC.

Background: Telomerase reverse transcriptase (TERT) is a catalytic subunit of telomerase and required for cancer development. This study aims to reveal its clinical utility for diagnosis and prognosis of resectable NSCLC.

Methods: TERT was quantitatively evaluated by the enzyme-linked immunosorbent assay (ELISA) from 69 patients before and after the surgery.

Pyruvate Abundance Confounds Aminoglycoside Killing of Multidrug-Resistant Bacteria via Glutathione Metabolism.

To explore whether the metabolic state reprogramming approach may be used to explore previously unknown metabolic pathways that contribute to antibiotic resistance, especially those that have been neglected in previous studies, pyruvate reprogramming was performed to reverse the resistance of multidrug-resistant . Surprisingly, we identified a pyruvate-regulated glutathione system that occurs by boosting glycine, serine, and threonine metabolism. Moreover, cysteine and methionine metabolism played a key role in this reversal.

Designing cobalt-nickel dual-atoms on boron, nitrogen-codoped carbon nanotubes for carbon dioxide electroreduction to syngas.

Developing highly efficient electrocatalysts to produce syngas with a stable hydrogen/carbon monoxide (H/CO) ratio in a wide potential window via electrochemical carbon dioxide (CO) reduction is desperately required but still challenging. Herein, a dual-atomic site on boron, nitrogen-codoped carbon nanotubes (BCN) has been designed, containing both cobalt (CoN) and nickel (NiNB) sites. Benefiting from the structure advantage and the bifunctional Co/Ni sites, such designed catalyst (CoNi-BCN) demonstrates remarkable performance for syngas production, achieving a stable H/CO ratio of 1.

Comparative analysis of complications and technique survival in peritoneal dialysis catheter insertion: single purse-string suture vs. double purses-string suture.

Objective: Single- and double-purse-string suture methods are both widely used in open surgical catheterization for peritoneal dialysis. This study aimed to compare the post-insertion complications and technical survival of the two methods.

Methods: This retrospective study matched 142 patients who underwent peritoneal catheterization using the single- (Group S) or double- (Group D) purse-string suture method.

Triglycerides and HDL Cholesterol Mediate the Association Between Waist Circumference and Hyperuricemia in Normal-Weight Men.

Purpose: Hyperuricemia has traditionally been associated with obesity and dyslipidemia. However, the relationship between waist circumference (WC) and hyperuricemia in normal-weight men is still unclear, particularly regarding the roles of triglycerides (TG) and high-density lipoprotein cholesterol (HDL-C). The aim of this research was to explore the mediating roles of TG and HDL-C in the association between WC and hyperuricemia in normal-weight men.

Longitudinal Analysis of Antibody Dynamics in Severe Fever with Thrombocytopenia Syndrome Patients - High-Incidence Regions of China, 2010-2023.

What Is Already Known About This Topic?: Severe fever with thrombocytopenia syndrome (SFTS) is a serious tick-borne disease in East Asia with high mortality, particularly affecting the elderly. Since its discovery in 2010, inconsistencies in small-scale studies and the lack of decade-long research on antibody levels in large population samples after natural infection, along with the absence of an effective vaccine, highlight the need for large-scale, long-term data in high-incidence regions of China.

What Is Added By This Report?: This study of 1,410 serum samples from SFTS patients in high-incidence regions of China reveals that immunoglobulin M (IgM) levels peak at 8-14 days post-infection, declining to nearly undetectable levels by 180 days.

Exploring Ion Transmission Mechanisms in Clay-Based 2D Nanofluidics for Osmotic Energy Conversion.

Clay-based 2D nanofluidics present a promising avenue for osmotic energy harvesting due to their low cost and straightforward large-scale preparation. However, a comprehensive understanding of ion transport mechanisms, and horizontal and vertical transmission, remains incomplete. By employing a multiscale approach in combination of first-principles calculations and molecular dynamics simulations, the issue of how transmission directions impact on the clay-based 2D nanofluidics on osmotic energy conversion is addressed.

Rice Under Dry Cultivation-Maize Intercropping Improves Soil Environment and Increases Total Yield by Regulating Belowground Root Growth.

Under the one-season-a-year cropping pattern in Northeast China, continuous cropping is one of the main factors contributing to the degradation of black soil. Previous studies (on maize-soybean, maize-peanut, and maize-wheat intercropping) have shown that intercropping can alleviate this problem. However, it is not known whether intercropping is feasible for maize and rice under dry cultivation, and its effects on yield and soil fertility are unknown.

Prediction method of surface subsidence induced by block caving method based on UAV oblique photogrammetry.

Unmanned Aerial Vehicle (UAV) oblique photogrammetry has been extensively employed in mining, albeit predominantly for reconstructing three-dimensional scenes and detecting changes within mining sites, lacking predictive capabilities. Leveraging 3D real scene model data, this study presents a two-stage prediction model, merging the probabilistic integral method with recurrent neural network (PIMF-RNN), to mitigate the impact of internal and external factors on surface subsidence, thereby enhancing predictive accuracy. Building upon this framework, a methodology was developed to forecast the maximum surface subsidence height and affected area under the block caving method, offering crucial data support for mitigating hazards associated with this mining technique.

Circulating exosomal protein EFEMP1 and SERPINC1 as diagnostic biomarkers for epithelial ovarian cancer.

Objectives: Caner-derived exosomes, containing diverse nucleic acids and proteins, are being exploited in diagnostic biomarker development. This study aims to screen and identify the altered exosomal proteins between epithelial ovarian cancer (EOC) patient and healthy volunteers, and to evaluate their diagnostic accuracy for EOC.

Methods: Exosomes were separate by ultracentrifugation, and then subjected to TEM, qNano, and western blot for identification.

Impact of bladder size and shape on the accuracy of formula method for bladder volume evaluation.

Background: To explore the impact of bladder size and shape on the accuracy of the formula method (V = 0.52 × d × d × d) for bladder volume evaluation.

Methods: Data was retrospectively collected from 220 patients without reportable bladder diseases.

Control of DNA demethylation by superoxide anion in plant stem cells.

Superoxide anion is thought to be a natural by-product with strong oxidizing ability in all living organisms and was recently found to accumulate in plant meristems to maintain stem cells in the shoot and undifferentiated meristematic cells in the root. Here we show that the DNA demethylase repressor of silencing 1 (ROS1) is one of the direct targets of superoxide in stem cells. The Fe-S clusters in ROS1 are oxidized by superoxide to activate its DNA glycosylase/lyase activity.

Circulating Exosomal Transfer RNA-Related Fragments as Diagnostic Biomarkers for Non-small Cell Lung Cancer.

Purpose: Exosomal transfer RNA-derived fragments [exo-tRF] possess the capacity to be employed as biomarkers for several types of cancer. We aim to ascertain the diagnostic significance of exosomal 5'tRF-TyrGTA and 5'tRF-ValTAC in non-small cell lung cancer [NSCLC].

Methods: Ultracentrifugation was deployed to obtain serum exosomes from NSCLC patients and healthy donors.

Straw Incorporation with Exogenous Degrading Bacteria (ZJW-6): An Integrated Greener Approach to Enhance Straw Degradation and Improve Rice Growth.

Rice straw is an agricultural waste, the disposal of which through open burning is an emerging challenge for ecology. Green manufacturing using straw returning provides a more avant-garde technique that is not only an effective management measure to improve soil fertility in agricultural ecosystems but also nurtures environmental stewardship by reducing waste and the carbon footprint. However, fresh straw that is returned to the field cannot be quickly decomposed, and screening microorganisms with the capacity to degrade straw and understanding their mechanism of action is an efficient approach to solve such problems.

Intrapuparial stage aging and PMI estimation based on the developmental transcriptomes of forensically important (Diptera: Calliphoridae) gene expression.

Background: The expression profiles of differentially expressed genes (DEGs) during pupal development have been demonstrated to be vital in age estimation of forensic entomological study. Here, using forensically important (Diptera: Calliphoridae), we aimed to explore the potential of intrapuparial stage aging and postmortem interval (PMI) estimation based on characterization of successive developmental transcriptomes and gene expression patterns.

Methods: We collected pupae at 11 successive intrapuparial stages at 20 °C and used the RNA-seq technique to build the transcriptome profiles of their intrapuparial stages.

Alteration in lipid metabolism is involved in nitrogen deficiency response in wheat seedlings.

Changes of membrane lipid composition contribute to plant adaptation to various abiotic stresses. Here, a comparative study was undertaken to investigate the mechanisms of how lipid alteration affects plant growth and development under nitrogen (N) deficiency. Two wheat cultivars: the N deficiency-tolerant cultivar Xiaoyan 6 (XY) and the N deficiency-sensitive cultivar Aikang 58 (AK) were used to test if the high N-deficiency tolerance was related with lipid metabolism.

Seasonal impact of diurnal temperature range on intracerebral hemorrhage in middle-aged and elderly people in central China.

Objectives: This study investigated the seasonal impact of diurnal temperature range (DTR) on hospitalization rates for intracerebral hemorrhage (ICH) in middle-aged and elderly adults.

Methods: We collected data on the DTR and hospitalization records of ≥45-year-old patients with ICH in 2019 in Hunan Province, central China. Time-series analyses were performed using a distributed lag non-linear model.