HPClas: A data-driven approach for identifying halophilic proteins based on catBoost.

Halophilic proteins possess unique structural properties and show high stability under extreme conditions. This distinct characteristic makes them invaluable for application in various aspects such as bioenergy, pharmaceuticals, environmental clean-up, and energy production. Generally, halophilic proteins are discovered and characterized through labor-intensive and time-consuming wet lab experiments.

Computation-Aided Development of Next-Generation Extractants for Trivalent Actinide and Lanthanide Separation.

The chemical similarities between trivalent actinides [An(III)] and lanthanides [Ln(III)] present a significant challenge in differentiating and separating them, which is a key step toward closing the nuclear fuel cycle. However, the existing separation approaches commonly suffer from demerits such as inadequate separation factors, limited stripping efficiency, and undesired coextraction. In this study, a novel unsymmetrical phenanthroline-derived amide-triazine (Et-Tol-CyMe-ATPhen) extractant was first designed and then screened with theoretical computation.

Validation of a Novel Strategy for Fluorescence Quenching for a Self-Quenching Fluorogenic Probe and Its Application for Visual Loop-Mediated Isothermal Amplification Detection During Food Safety Analysis.

The self-quenching fluorogenic probe facilitates precise identification of LAMP (loop-mediated isothermal amplification) amplicons, unaffected by non-specific products resulting from primer dimers. However, low quenching efficiency by surrounding nucleobases leads to high background signal, posing significant challenges for visual inspection with the naked eye. The present study aims to identify an oligonucleotide sequence that is complementary to the self-quenching fluorogenic probe, and to employ the fluorescence super-quenching mechanism of double-stranded DNA to establish a visualization system for the LAMP assay.

Extraction and Complexation of Fission Products with Unsymmetrical Phenanthroline Diamide Ligands from Simulated High-Level Liquid Waste.

The extraction performance and complexation properties between some typical fission products (FPs) with phenanthroline-based ligands ,,-triethyl--tolyl-1,10-phenanthroline-2,9-dicarboxamide (DE-ET-DAPhen) and -ethyl-,-dioctyl--tolyl-1,10-phenanthroline-2,9-dicarboxamide (DO-ET-DAPhen) were described in this work. The low distributions of Ln(III) observed in the solvent extraction study showed the potential of the ligands for the separation of actinides and lanthanides in high-level liquid waste (HLLW). Further extraction studies on other FPs showed that the ligands could efficiently extract Pd(II) and Cd(II) using -octanol as a diluent.

Investigating the mechanism of Qifu Yin in ameliorating memory disorders through pseudo-targeted lipidomics.

Memory disorder (MD) is a neurodegenerative disease with an increasing incidence rate that adversely affects the quality of life of patients. Qifu Yin (QFY), a classic traditional Chinese medicine formula used for treating dementia, is known for its neuroprotective properties, although its mechanism of action requires further exploration. In this study, D-galactose combined with aluminum chloride was used to establish an MD rat model, and behavior, histopathology, and related indicators were used to evaluate the pharmacodynamics of the formula in the rats.

Uneven distribution of prokaryote-derived horizontal gene transfer in fungi: a lifestyle-dependent phenomenon.

Unlabelled: Horizontal gene transfer (HGT) in fungi is less understood despite its significance in prokaryotes. In this study, we systematically searched for HGT events in 829 representative fungal genomes. Using a combination of sequence similarity and phylogeny-based approaches, we detected 20,093 prokaryotic-derived transferred genes across 750 fungal genomes, via 8,815 distinct HGT events.

Computed tomography enterography-based deep learning radiomics to predict stratified healing in patients with Crohn's disease: a multicenter study.

Objectives: This study developed a deep learning radiomics (DLR) model utilizing baseline computed tomography enterography (CTE) to non-invasively predict stratified healing in Crohn's disease (CD) patients following infliximab (IFX) treatment.

Methods: The study included 246 CD patients diagnosed at three hospitals. From the first two hospitals, 202 patients were randomly divided into a training cohort (n = 141) and a testing cohort (n = 61) in a 7:3 ratio.

Physiological and Genetic Aspects of Resistance to Abiotic Stresses in Species.

Abiotic stress is one of the key factors harming global agriculture today, seriously affecting the growth and yield of vegetables. Pepper is the most widely grown vegetable in the world, with both high nutritional and economic values. Currently, the increase in global extreme weather events has heightened the frequency of abiotic stresses, such as drought, high and low temperatures, waterlogging, and high salt levels, which impairs pepper growth and development, leading to its reduced yield and quality.

A Novel Method for Rapid Screening of Salmonidae Ingredients and Accurate Detection of Atlantic Salmon () Simultaneously Using Duplex Real-Time PCR Coupled with Melting Curve Analysis.

The substitution of ingredients with Salmonidae, particularly , has led to widespread reports of financial losses and health risks globally, emphasizing the urgent need for the development of a rapid and precise method for species identification. The aim of the present study was to develop a novel method for the rapid screening of Salmonidae ingredients and the accurate detection of simultaneously using multiplex real-time PCR coupled with melting curve analysis. Specifically, primer sets specific for and Salmonidae were cross-confirmed.

Bidirectional enhancement of output performance of nanogenerators by M-COFs materials.

The emergence of nanogenerators, which have the ability to capture mechanical energy from the environment and to collect and transmit tiny energy, is rapidly becoming a hot research topic. The performance of electrode materials is the key to the efficiency of nanogenerators. Covalent organic skeletons (COFs), a class of crystalline organic porous materials with the advantages of large specific surface area, high porosity, tunable structure, and flexible tailorability, have very significant advantages in being used as nanogenerator materials.

Efficient Adsorption and Degradation of Tetracycline Hydrochloride Using Metal-Organic Framework-Derived Cobalt-Based Catalysts and Mechanism Insight.

High-performance Co-based catalysts were derived by pyrolysis using synthesized MOFs as self-sacrificial templates. Various catalytic systems were constructed by peroxymonosulfate (PMS) to degrade tetracycline hydrochloride (TC). Adsorption-degradation efficiencies, cycle performance, dynamics, and the adsorption catalytic mechanism of various catalytic systems for TC were studied.

Effects of low temperature air exposure and immersion on antioxidant, immune, intestinal flora and metabolome of Chinese mitten crab (Eriocheir sinensis).

The aim of this study was to investigate the effects of immersion on immune enzyme activity, haemolymph index, intestinal microbiome and metabolome of E. sinensis after low temperature air exposure. The results showed that low temperature air exposure induced stress response, which led to hepatopancreas injury and increased membrane permeability, but this situation was reversible and alleviated after immersion.

Global Transcriptomic and Characteristics Comparisons between Mouse Fetal Liver and Bone Marrow Definitive Erythropoiesis.

Erythropoiesis occurs first in the yolk sac as a transit "primitive" form, then is gradually replaced by the "definitive" form in the fetal liver (FL) during fetal development and in the bone marrow (BM) postnatally. While it is well known that differences exist between primitive and definitive erythropoiesis, the similarities and differences between FL and BM definitive erythropoiesis have not been studied. Here we performed comprehensive comparisons of erythroid progenitors and precursors at all maturational stages sorted from E16.

Effects of dietary ginger (Zingiber officinale) polysaccharide on the growth, antioxidant, immunity response, intestinal microbiota, and disease resistance to Aeromonas hydrophila in crucian carp (Carassius auratus).

Ginger polysaccharides (GP) promote growth and development in fish. However, the effects of GP on crucian carp remain unclear. The present study investigated the effects of GP on the growth performance, immunity, intestinal microbiota, and disease resistance in crucian carp.

Four new species of from the Xizang Autonomous Region and other provinces of China.

is the largest genus in the Russulales and is widespread throughout the world. Almost all species are known to be ectomycorrhizal with high ecological and edible values, and some are lethal poisonous. In this study, four new species belonging to the subgenus crown clade are identified based on morphological and phylogenetic evidence from the Xizang Autonomous Region and other provinces of China.

Hexadecanoic acid produced in the co-culture of S. cerevisiae and E.coli promotes oxidative stress tolerance of the S.cerevisiae cells.

Co-fermentation performed by Saccharomyces cerevisiae and Escherichia coli or other microbes has been widely used in industrial fermentation. Meanwhile, the co-cultured microbes might regulate each other's metabolisms or cell behaviors including oxidative stress tolerance through secreting molecules. Here, results based on the co-culture system of S.

[Establishment and evaluation of a rabbit model of frozen shoulder induced by persistent strain injuries and ice compression].

Objective: To evaluate the rabbit modle of frozen shoulder induced by persistent strain injuries and ice compression.

Methods: Twelve clean, healthy male New Zealand rabbits with a mass of (2 500±500) g were selected and randomly divided into a blank group and a control group with 6 rabbits in each group. In the control group, the rabbits were modeled with persistent strain injuries and ice compression, the general conditions of the rabbits and the active and passive activities of the shoulder joint were observed and their body weights were recorded.

Epimerization effects on coordination behaviours of phenanthroline-based phosphine-oxide ligands with uranyl ions.

Epimers of the (1,10-phenanthroline-2,9-diyl)bis(ethyl(phenyl)phosphine oxide) (Et-Ph-BPPhen) ligand with two chiral centers (,/, and ,) were synthesized. The configurational effects on the coordination ability and mechanism between these epimeric ligands and uranyl ions were thoroughly investigated. This work is helpful to reveal the effects of different conformations of epimeric ligands on their coordination properties.

Untargeted LC-MS metabolomics reveals the metabolic responses in the Eriocheir sinensis gills exposed to salinity and alkalinity stress.

In recent years, saline-alkaline aquaculture development has become an important measure for China to expand its fishery development space to ensure food safety. Previous studies have verified that salinity and alkalinity positively influence the quality of Chinese mitten crabs (Eriocheir sinensis). However, the regulatory mechanism of E.

[Analysis of signal peptides and secreted proteins in the whole proteome of ].

The signal peptide is a key factor that affects the efficiency of protein secretion in . Currently, the most used signal peptide is the α-mating factor (MFα) pre-pro leader from . This exogenous signal peptide has been successfully utilized to express and secret many heterologous proteins.