The study of routine laboratory factors in children with mycoplasma pneumoniae pneumonia: serum uric acid may have anti-inflammatory effect.

Background: High uric acid levels are a risk factor for cardiovascular disorders, and metabolic diseases; however, the role of serum uric acid (sUA) during the mycoplasma pneumoniae pneumonia (MPP) of children is poorly known. This study aimed to clarify the effects of sUA during the MPP of children.

Methods: This was a prospective cohort study of children with MPP from multi-center inpatient departments from September 2019 to August 2020.

ITPKA induces cell senescence, inhibits ovarian cancer tumorigenesis and can be downregulated by miR-203.

Overcoming senescence is a feature of ovarian cancer cells; however, the mechanisms underlying senescence regulation in ovarian cancer cells remain largely unknown. In this study, we found that ITPKA was downregulated in ovarian cancer samples, and the lower expression correlated with poor survival. Overexpression of ITPKA inhibited the anchorage-independent growth of ovarian cancer cells and induced senescence.

[Investigation and morphological observation of breeding in flourmills in Huaibei].

Objective: To investigate the breeding in the flourmill and further observe its morphological characteristics.

Methods: The samples were collected from different habitats of a flourmill and then separated, and the chipping was kept. The mites were selected and counted from the chipping under an optical microscope.

[Optimization and comparison of extraction methods of mitochondrial DNA of ].

Objective: To optimize the extraction methods of mitochondrial genome DNA (mtDNA) of .

Methods: The pyrolysis, protein K variable-temperature digestion and high-concentration potassium acetate purification were applied to optimize the high-concentration-salt precipitation method, and then the optimized method was compared with two common extraction methods, the sucrose density gradient centrifugation method and traditional high-concentration-salt precipitation method. The mtDNA samples were identified by using spectrophotometry, agarose gel electrophoresis and the amplification products of COX1.

[Preliminary investigation of breeding status in ground dust flour in Qiqihaer City].

Objective: To investigate the breeding status in the ground dust flour collected in Qiqihaer City.

Methods: Totally, 16 aliquots of ground dust flour were respectively collected from a college canteen and 15 households in Qiqihaer City. Then 10 g dust flour was taken from individual sample for isolation of the mites that were made of slide specimen, and the mites were identified and classified under a microscope.

MiR-203 promotes the growth and migration of ovarian cancer cells by enhancing glycolytic pathway.

MicroRNAs (miRNAs) play an important role in the tumorigenesis of ovarian cancer. Previously, we have reported the dysregulation of miR-203 in the ovarian cancer tissues. However, the biological functions and molecular mechanisms of miR-203 in ovarian cancer remain unknown.

[Exploration on the Technology of Counter Staining of Fluke].

Fixed samples of Clonorchis sinensis and Fasciolopsis buski were stained with acetocarmine and malachite green, or stained with acetocarmine only. The samples displayed three different colors after staining with acetocarmine and malachite green. The digestive system, excretory system and the surrounding muscle tissue were stained reddish, the uterus was bright green, and the vitellarium at each side of the worm was tan.

[Population dynamics and spatial distribution pattern of ].

Objective: To characterize the population dynamics and spatial distribution of in the flour warehouse, so as to provide the basic evidence for improving the sampling guidelines that are essential for effective pest monitoring and management.

Methods: The samples from flour warehouses of four localities were collected, examined and counted for in every month in Wuhu City. The dispersion pattern target, Iwao / regression analysis and Taylor power method were used for analyzing the spatial distribution pattern of in the flour warehouses.

[Investigation on Aspidogastrea infection in freshwater mussels in Anhui Province].

Objective: To investigate the prevalence of trematode Aspidogastrea in freshwater mussels in Anhui Province, China.

Methods: The freshwater mussels living in different water areas within Anhui territory were harvested and dissected from April to May, 2015. Then the flukes were collected from the pericardial cavities of the clams into a petri dish containing small amount of saline.

[ and its hypopus found in feed of ].

Objective: To observe the and its hypopus in feed of .

Methods: Feed samples were collected from farms, and and its hypopus were separated from the samples by the vibration sieve method and floating method. The glass specimens were prepared and and its hypopus were observed under an optical microscope.

[Investigation on species and community ecology of Acaroid mites breeding in stored traditional Chinese animal medicinal materials].

Objective: To investigate the species of Acaroid mites breeding in the stored traditional Chinese animal medicinal materials and the relationship between its community and habitats.

Methods: A total of 30 samples of traditional Chinese animal medicinal herbs were collected from Wuhu City, Anhui Province, China. The mites were isolated by the directly microscopic and floatation microscopic examinations, and then identified and counted under a light microscope.

[Investigation on mites and insects breeding status of bed mats in college dormitories in Wuhu City].

Objective: To understand the breeding situations of mites and insects from bed mats in dormitories in a college in Wuhu City, so as to provide evidences for improving the prevention and control of mites.

Methods: From March to May, 2015, the dust samples from bed mats in student dormitories were collected and detected for mites and insects by microscopy. In addition, the intervention measures including soaking the mats in the warm water, exposing the mats to the sunlight for a long time and cleaning up the indoor environment were carried out, and the breeding situations of mites and insects before and after the intervention were compared.

[Investigation on Cheyletoidea mites breeding in culture environment of and morphologic observation of Cunliffe].

Objective: To investigate the species of Cheyletoidea mites breeding in the culture environment of and to observe the morphology of Cunliffe.

Methods: The soil samples from an farm in northern Anhui were collected. The mites in the soil samples were separated directly under a microscope and the glass specimens were made to observe the morphological feature of the mites under a light microscope, then the mites species were identified and classified based on the morphological characteristics.

[Metorchis orientalis founded in basin of Wuhu City, Anhui Province].

Objective: To investigate the epidemic situation of Metorchis orientalis in Wuhu City, Anhui Province.

Methods: Three lakes (Fengming, Longwo and Kui lakes) were selected in Wuhu City, and the poultries around the lakes and freshwater fishes in the lakes were captured to test the infection of M. orientalis, and the infection rates were calculated.

[Composition and diversity of Acaroid mites' communities in indoor environments of kindergartens in Wuhu City].

Objective: To understand the breeding situation of Acaroid mites in indoor environments of kindergartens in Wuhu City, so as to provide the evidence for its prevention and control.

Methods: From March to June and September to December in 2014, dust samples were collected from 15 kindergartens of 3 ranks every month. Acaroid mites in the samples were isolated, identified and counted.

HISTOPATHOLOGY OF GASTRIC WALL IN CHINESE ALLIGATOR ALLIGATOR SINENSIS INFECTED WITH ORTLEPPASCARIS SINENSIS (NEMATODA: ASCARIDOIDEA).

Crocodiles are susceptible to infection with a wide array of external and internal gastrointestinal helminths, yet little is known on the histopathology following infection or the effects of these parasites. The present study was aimed at evaluating the impact of infection by Ortleppascaris sinensis (Nematoda: Ascaridoidea) on the stomach of captive Alligator sinensis. The histological examination of the stomach revealed presence of superficial ulcer in mucous layer and granulomatous inflammation in submucous layer at entire gastric walls of the Alligator sinensis.

[Adult of Drosophila melanogaster parasitized in human nasal cavity: a case report].

We reported a case of adult Drosophila melanogaster parasitized in nasal cavity of a 81-year-old woman who was living in Xuancheng City, Anhui Province now. She was admitted for treatment of cerebral infarction and water accumulation in the lungs in 2014 June. The patient was also suffering from secretory otitis media, a history of hypertension and heart stents were placed in 2007.

[Experimental study on determination of viability of Schistosoma japonicum cercariae by staining].

Objective: To determine the viability of Schistosoma japonicum cercariae by staining.

Methods: Schistosoma japonicum cercariae were stained by 0.4% trypan blue, 0.

Preliminary report on the intestinal parasites and their diversity in captive Chinese alligators.

Objective: Although intestinal parasites are commonly detected in either wild or farmed crocodiles in other parts of the world, yet few reports have ever been on the captive bred Chinese alligators (Alligator sinensis) since they are only distributed in the southern areas of the Yangtze River. The current work was undertaken to investigate the intestinal parasites and their diversity in the Chinese crocodilian species.

Methods: In May and October of 2013, we randomly collected a total of 328 fecal samples of the captive Chinese alligators in a breeding centre in south Anhui province.

EMSY promoted the growth and migration of ovarian cancer cells.

Epithelial ovarian cancer is one of the most common and aggressive diseases among the female reproductive organ malignancies, and the molecular mechanism underlying this disease remains largely unknown. EMSY, a binding partner of BRCA2, has been reported to be amplified in ovarian cancer. However, the expression pattern and biological functions of EMSY in the progression of ovarian cancer are not fully understood.

[Experimental study on quick determination of viability of Oncomelania hupensis by staining].

Objective: To find out a quick, simple and convenient method of determining the viability of Oncomelania hupensis.

Methods: O. hupensis snails were stained for 30 minutes by 0.

[Investigation on species and community ecology of cheyletoid mites breeding in the stored traditional Chinese medicinal materials].

Objective: To investigate the species of Cheyletoid mites breeding status in the stored traditional Chinese medicinal materials and the relationship between its community and habitats.

Methods: A total of 60 samples of the traditional Chinese medicinal materials were collected from Huainan, Wuhu and Xuancheng in China. The mites were isolated with shakesieve shock, directicopy, Tullgren funnel, waternacopy and redricopy, and identified and counted under the light microscope.

[Killing effect of exogenous NO on cercariae of Schistosoma japonicum in vitro].

Objective: To explore the killing effects of exogenous NO on the cercariae of Schistosoma japonicum in vitro and the blocking effects of NO inhibitors.

Methods: The cercariae of S. japonicum were collected from naturally infected snails, and then formulated into a 1000 cercariae/ml suspension with RPMI 1640 medium.

Upregulation of microRNA-203 is associated with advanced tumor progression and poor prognosis in epithelial ovarian cancer.

MicroRNA-203 (miR-203), possessing tumor suppressive or promotive activities, has been found to be downregulated or upregulated in different cancer types. The purpose of this study was to investigate whether the increased expression of miR-203 can be used as a noninvasive diagnostic and prognostic biomarker in epithelial ovarian cancer (EOC). Real-time quantitative PCR was performed to detect the expression levels of miR-203 in EOC tissues.

[Reform and practice on the experiment teaching of medical parasitology].

A new model of education is investigated to meet the new idea of experiment teaching in university. Therefore the establishment of experiment teaching model of medical parasitology needs to be correspondently reformed. A variety of new management measures are taken to raise the efficiency of experiment teaching in training the students in the College.