ST11 Carbapenem-Resistant Klebsiella pneumoniae Clone Harboring Capsular Type KL25 became the primarily prevalent capsular serotypes in a Tertiary Teaching Hospital in China.

Objective: The spread of carbapenem-resistant Klebsiella pneumoniae (CRKP) that produces Klebsiella pneumoniae carbapenemase-2 (KPC-2) has garnered significant attention due to their increasing prevalence. In China, the dissemination of KPC-2-producing CRKP is predominantly linked to sequence type 11 (ST11), with the capsular serotypes KL47 and KL64 being the primary capsular identified.

Methods: Sixty-four CRKP-ST11 strains were collected from 2020 to 2023.

A novel bHLH transcription factor, FabHLH110, is involved in regulation of anthocyanin synthesis in petals of pink-flowered strawberry.

The pink-flowered strawberry is a kind of perennial herb that serves as both an ornamental plant with a range of red-colored petals and a food crop, which was produced by distant hybridization of Fragaria × Potentilla. Although there have been numerous reports on anthocyanin synthesis in strawberry fruits, the mechanism by which bHLH transcription factors regulate anthocyanin synthesis in strawberry red petals remains unclear. In this study, a total of 376 FabHLHs were finally identified, which were divided into 25 subfamilies.

Gene regulatory network inference during cell fate decisions by perturbation strategies.

With rapid advances in biological technology and computational approaches, inferring specific gene regulatory networks from data alone during cell fate decisions, including determining direct regulations and their intensities between biomolecules, remains one of the most significant challenges. In this study, we propose a general computational approach based on systematic perturbation, statistical, and differential analyses to infer network topologies and identify network differences during cell fate decisions. For each cell fate state, we first theoretically show how to calculate local response matrices based on perturbation data under systematic perturbation analysis, and we also derive the wild-type (WT) local response matrix for specific ordinary differential equations.

Multiomics provides insights into dynamic changes of aromatic profile during flue-curing process in tobacco (Nicotiana tabacum L.) leaves.

Tobacco (Nicotiana tabacum L.) is a globally crop due to its distinctive flavor and economic value. In this study, we systematically analyzed the dynamic changes in volatile substances, broad-spectrum metabolites, enzymes, and biochemical compounds in tobacco leaves during flue-curing process.

Harnessing Membrane Coating for Probiotic Therapy in Inflammatory Bowel Disease.

Inflammatory bowel diseases (IBDs) are characterized by dysregulated inflammatory responses, increased reactive oxygen species (ROS), and compromised intestinal barrier function. Current clinical treatments often yield suboptimal responses and significant side effects. Given the great potential of (AKK) bacterial membrane components in IBD treatment and the possible pathogenicity of live bacteria, to essentially address these challenges, we develop a natural membrane-based single-cell coating, incorporating a self-assembled AKK bacterial membrane (AKM) and astaxanthin (AST), forming a protective biological barrier on probiotics.

Deciphering the heterogeneity and plasticity of the tumor microenvironment in liver cancer provides insights for prognosis.

Liver cancer exhibits diverse molecular characteristics and distinct immune cell infiltration patterns, which significantly influence patient outcomes. In this study, we thoroughly examined the liver cancer tumor environment by analyzing data from 419,866 individual cells across nine datasets involving 99 patients. By categorizing patients into different groups based on their immune cell profiles, including immune deficiency, B cells-enriched, T cells-enriched and macrophages-enriched, we better understood how these cells change in various patient subgroups.

Recombinant human thrombopoietin does not promote platelet engraftment in newly diagnosed multiple myeloma patients following autologous stem cell transplantation.

To evaluate the effect of recombinant human thrombopoietin (rhTPO) on platelet engraftment after autologous stem cell transplantation (ASCT) in newly diagnosed multiple myeloma (NDMM) patients. Clinical data of 40 NDMM patients who underwent high-dose melphalan conditioning and ASCT were analyzed, with 26 receiving rhTPO and 14 in the control group. The primary study endpoint was the median time to platelet engraftment.

Prediction of a radiomics-based nomogram for complete remission in atypical endometrial hyperplasia and early endometrial cancer patients seeking fertility preservation.

Fertility-sparing treatments are becoming increasingly important for patients worldwide with atypical endometrial hyperplasia (AEH) and early endometrial cancer (EC). The primary aim of these treatments is to achieve complete regression (CR). Accurately predicting CR and applying effective treatment strategies throughout the process are crucial for successful fertility preservation in these patients.

Tribbles Pseudokinase 3 Converts Sorafenib Therapy to Neutrophil-Mediated Lung Metastasis in Hepatocellular Carcinoma.

Rapid development of resistance to sorafenib and subsequent hyperprogression in patients with advanced hepatocellular carcinoma (HCC) pose significant challenges, with the underlying mechanisms still largely unknown. Herein, sorafenib-induced TRIB3 is identified as a liver-specific determinant driving secondary resistance to sorafenib by facilitating the accumulation of protumorigenic neutrophils within tumors. Mechanistically, TRIB3, triggered by the sorafenib-elicited ROS-ER stress axis, operates in an NF-κB-dependent manner to upregulate CXCR1/2 ligands, subsequently promoting neutrophil recruitment into tumors.

Anti-neuroinflammatory agent rhein lysinate-based self-assembled injectable hydrogel loaded with ZL006 for promoting post-stroke functional recovery.

The therapeutic agent-based self-assembled hydrogel is gaining interest for biomedical applications, because it overcomes the poor biodegradability and low therapeutic agent loading of conventional polymer gelator-based hydrogel. Here, we present rhein lysinate (RHL), a therapeutic agent that self-assembles to form a stable hydrogel through the π-π stacking and hydrogen bonding interactions, while also exerting anti-neuroinflammatory effect. As a small molecular hydrogelator, RHL has significantly improved water solubility and enhanced self-assembly and gelation capabilities compared to the natural anthraquinone rhein.

aux orchestrates the phosphorylation-dependent assembly of the lysosomal V-ATPase in glia and contributes to SNCA/α-synuclein degradation.

Glia contribute to the neuropathology of Parkinson disease (PD), but how they react opposingly to be beneficial or detrimental under pathological conditions, like promoting or eliminating SNCA/α-syn (synuclein alpha) inclusions, remains elusive. Here we present evidence that aux (auxilin), the homolog of the PD risk factor GAK (cyclin G associated kinase), regulates the lysosomal degradation of SNCA/α-syn in glia. Lack of glial GAK/aux increases the lysosome number and size, regulates lysosomal acidification and hydrolase activity, and ultimately blocks the degradation of substrates including SNCA/α-syn.

High anti-Müllerian hormone level as a predictor of poor pregnancy outcomes in women with polycystic ovary syndrome undergoing in vitro fertilization/intracytoplasmic sperm injection: a retrospective cohort study.

Objective: To study the correlation between anti-Müllerian hormone levels and pregnancy outcomes after in vitro fertilization/intracytoplasmic sperm injection in women with polycystic ovary syndrome, which remains controversial.

Methods: This retrospective cohort study recruited 4,719 women with infertility and polycystic ovary syndrome aged 20-40 years who underwent treatment at the Reproductive Center of Peking University Third Hospital between February 2017 and June 2023. We divided the participants into three groups according to the 25th and 75th percentile cutoffs of serum anti-Müllerian hormone: low (≤ 4.

Correction: Self-generating electricity system driven by aqueous humor flow and trabecular meshwork contraction motion activated BKCa for glaucoma intraocular pressure treatment.

Correction for 'Self-generating electricity system driven by aqueous humor flow and trabecular meshwork contraction motion activated BKCa for glaucoma intraocular pressure treatment' by Ruiqi Wang , , 2025, https://doi.org/10.1039/D4MH01004C.

Obesity and recurrent spontaneous abortion: the crucial role of weight management in pregnancy.

Recurrent spontaneous abortion (RSA), characterized by the loss of two or more pregnancies, impacts approximately 1-2% of couples and poses a significant challenge for individuals of childbearing age. The precise mechanisms underlying RSA remain incompletely understood. Concurrently, the global prevalence of obesity is on the rise, with obesity being closely associated with female reproductive disorders and infertility.

Identification of SETD4 as an Onco-Immunological Biomarker Encompassing the Tumor Microenvironment, Prognoses, and Therapeutic Responses in Various Human Cancers.

Background: SET domain-containing protein 4 (SETD4) is a histone methyltransferase that has been shown to modulate cell proliferation, differentiation, and inflammatory responses by regulating histone H4 trimethylation (H4K20me3). Previous reports have demonstrated its function in the quiescence of cancer stem cells as well as drug resistance in several cancers. A limited number of systematic studies have examined SETD4's role in the tumor microenvironment, pathogenesis, prognosis, and therapeutic response.

AMPK: An energy sensor for non-small cell lung cancer progression and treatment.

Lung cancer (LC) is the leading cause of cancer-related morbidity and mortality in China, with non-small cell lung cancer (NSCLC) accounting for 85 % of the overall lung cancer cases. AMP-activated protein kinase (AMPK) is a key regulator of energy balance and homeostasis, and its dysregulation is a common feature in various malignancies, particularly in NSCLC with mutations in Liver kinase B1 (LKB1). Studies have shown that the AMPK signalling pathway has a dual role in NSCLC progression, both inhibiting and promoting the progression of malignant tumours.

Deletion of BMP4 impairs trophoblast function and decidual macrophage polarization via autophagy leading to recurrent spontaneous abortion.

Bone morphogenetic protein 4 (BMP4) is widely involved in the regulation of cell proliferation and differentiation, but its role in Recurrent Spontaneous Abortion (RSA) remains unclear. RSA is a disease that affects roughly 1-2% of partner pairs, but its pathogenesis is still unclear. In recent years, many studies have focused on the role of decidual macrophages in RSA.

A hybrid dual-branch model with recurrence plots and transposed transformer for stock trend prediction.

Stock trend prediction is a significant challenge due to the inherent uncertainty and complexity of stock market time series. In this study, we introduce an innovative dual-branch network model designed to effectively address this challenge. The first branch constructs recurrence plots (RPs) to capture the nonlinear relationships between time points from historical closing price sequences and computes the corresponding recurrence quantifification analysis measures.

Genome-Wide Analysis of the PYL Gene Family in and Its Responses to Abiotic Stresses.

Abscisic acid (ABA) is a key phytohormone that participates in various plant biological processes, such as seed germination, senescence, stomatal movement, and flowering. In the ABA signal transduction pathway, Pyrabactin Resistance 1 (PYR1)/PYR1-Like (PYL)/Regulatory Component is the core module for ABA perception. In this study, a total of 12 PYL family members were identified in birch ( Suk.

The Pb tolerance initiated by LdZIP8 in Lymantria dispar larvae: An effective defense against heavy metal stress.

Pb is a prevalent heavy metal contaminant in the habitats of herbivorous insects. This study investigated the tolerance level of Lymantria dispar larvae to Pb and its corresponding mechanism focusing on the role of ZIP genes. The detrimental impacts of Pb on larval growth and survival exhibited a dose-dependent relationship, with a survival rate of 48 % even at the extreme concentration of 3424 mg/kg.

Differences in the efficiency and mechanisms of different iron-based materials driving synchronous nitrogen and phosphorus removal.

Iron-dependent denitrification has been substantially investigated worldwide due to the advantages of low cost, high efficiency, and synchronized phosphorous removal. However, differences in nitrogen metabolism processes with different iron-based materials as electron donors have not been systematically studied. This study investigated the efficacy of nitrogen and phosphate removal using various iron-based materials as electron donors.

Gut microbiota-derived 4-hydroxyphenylacetic acid from resveratrol supplementation prevents obesity through SIRT1 signaling activation.

Resveratrol (RSV), a natural polyphenol, has been suggested to influence glucose and lipid metabolism. However, the underlying molecular mechanism of its action remains largely unknown due to its multiple biological targets and low bioavailability. In this study, we demonstrate that RSV supplementation ameliorates high-fat-diet (HFD)-induced gut microbiota dysbiosis, enhancing the abundance of anti-obesity bacterial strains such as and .

Correlation between the mechanism of arteriopathy in IgA nephropathy and blood stasis syndrome: A cohort study.

To investigate the correlation between blood stasis syndrome and arteriopathy in immunoglobulin A nephropathy (IgAN). Wall thickness/outer vessel diameter, intimal thickness/outer vessel diameter, and medial thickness/outer vessel diameter were measured using ImageJ software. Vascular endothelial-derived growth factor (VEGF), matrix metalloproteinase-9 (MMP-9), proliferating cell nuclear antigen (PCNA), extracellular signal-regulated kinase (ERK) 1/2, and nuclear factor kappa B (NF-κB) were detected by immunohistochemical staining.

Long-term survival and failure patterns in inoperable early-stage non-small cell lung cancer following stereotactic body radiotherapy: a single-institution retrospective study.

This study is to analyse the failure patterns and long-term survival after stereotactic body radiotherapy (SBRT) in patients with T1-3N0M0 inoperable non-small cell lung cancer (NSCLC). Early-stage NSCLC patitents who received SBRT at Zhejiang Cancer Hospital from January 2012 to September 2018 were retrospectively analyzed. The primary endpoint were the patterns of disease progression, which were divided into local recurrence, regional failure, and distant metastasis.