NcSWP8, a New Spore Wall Protein, Interacts with Polar Tube Proteins in the Parasitic Microsporidia .

is a pathogen that affects and Fabricius, capable of spreading within and between honeybee colonies. The spore wall of microsporidia is the initial structure to contact the host cell directly, which may play a crucial role in the infection process. Currently, several spore wall proteins have been identified in microsporidia, but only two spore wall proteins from have been characterized.

MiR-125b-5p ameliorates ox-LDL-induced vascular endothelial cell dysfunction by negatively regulating TNFSF4/TLR4/NF-κB signaling.

Background: Oxidized low-density lipoprotein (ox-LDL)-induced endothelial cell dysfunction plays a crucial role in the progression of atherosclerosis (AS). Although miR-125b-5p is known to be involved in cardiovascular and cerebrovascular disorders, its function in ox-LDL-induced endothelial injury is still not well understood.

Methods: An in vitro AS cell model was established by exposing human umbilical vein endothelial cells (HUVECs) to 100 µg/mL ox-LDL for 24 h.

Transcriptional Profiling of Testis Development in Pre-Sexually-Mature Hezuo Pig.

Spermatogenesis is an advanced biological process, relying on intricate interactions between somatic and germ cells in testes. Investigating various cell types is challenging because of cellular heterogeneity. Single-cell RNA sequencing (scRNA-seq) offers a method to analyze cellular heterogeneity.

DNA Origami Framework-Based Spatial Nanochip for Circular ssDNA Assembly and Data Storage.

A 3D DNA spatial chip (DSC) based on an icosahedral DNA origami framework is introduced to construct customized circular single-stranded DNA (c-ssDNA) for data storage. Within the confined space of the DSC, thirty addressable location sequences extending from the framework edges are available for designing circular paths and directing the assembly of a series of information oligonucleotides for efficient ligation. This strategy is verified by constructing c-ssDNAs from up to 15 fragments to encode two poems (800 and 860 nucleotides).

A prospective and comparative study on improving the diagnostic accuracy of early gastric cancer based on deep convolutional neural network real-time diagnosis system (with video).

Background: Endoscopic diagnosis of early gastric cancer (EGC) is a challenge. It is not clear whether deep convolutional neural network (DCNN) model could improve the endoscopists' diagnostic performance.

Methods: We established a DCNN-assisted system and found that accuracy of diagnosis is higher than endoscopists.

Identification of scavenger receptor (LmSRA3) gene and its immune response to Aeromonas veronii in Lateolabrax maculatus.

Scavenger receptors (SRs) serve as essential pattern recognition receptors in the innate immune system, playing multiple roles in the immunity of fish. They contribute to defense mechanisms against pathogenic infections through various pathways. However, research on the functions of SRs in the immune response of Spotted sea bass remains limited.

Stereoretentive Conversion to -Glycosides from -Glycosides via Ligand-Coupling on Sulfur(IV).

A novel strategy is reported for the stereoselective synthesis of C(sp)-C(sp) -glycosides, which converts heteroaryl -glycosides into heteroaryl -glycosides with retention of configuration through a sequential process involving oxidation and Grignard reagent attack. The new method involves the generation of a S(IV) intermediate, followed by ligand coupling of the glycosyl and heteroaryl groups to yield heteroaryl -glycosides. The diverse heteroaryl -glycosides were achieved with good efficiency.

Electroacupuncture Pretreatment Reduces Ischemic Brain Injury by Inhibiting the Lactate Production and Its Derived Protein Lactylation Formation.

Aim: Given that electroacupuncture (EA) pretreatment inhibits lactate production and lactate-derived lysine lactation (Kla) aggravates ischemic brain injury, we aimed to investigate whether the formation of Kla protein is involved in EA pretreatment to alleviate ischemic brain injury.

Methods: EA was performed on the Baihui acupoint (GV20) of male C57BL/6J mice before receiving the permanent middle cerebral artery occlusion (pMCAO) surgery. Western blot and immunofluorescent staining were used to observe neuronal survival, astrocyte activation, and protein Kla levels, and the lactate levels in ischemic brains were assayed with a commercial kit.

Metabolic reprogramming and macrophage expansion define ACPA-negative rheumatoid arthritis: insights from single-cell RNA sequencing.

Background: Anti-citrullinated peptide antibodies (ACPA)-negative (ACPA-) rheumatoid arthritis (RA) presents significant diagnostic and therapeutic challenges due to the absence of specific biomarkers, underscoring the need to elucidate its distinctive cellular and metabolic profiles for more targeted interventions.

Methods: Single-cell RNA sequencing data from peripheral blood mononuclear cells (PBMCs) and synovial tissues of patients with ACPA- and ACPA+ RA, as well as healthy controls, were analyzed. Immune cell populations were classified based on clustering and marker gene expression, with pseudotime trajectory analysis, weighted gene co-expression network analysis (WGCNA), and transcription factor network inference providing further insights.

Critical review on emerging photocatalytic membranes for pollutant removal: From preparation to application.

Due to synergistically enhanced separation and degradation performances, photocatalytic membranes offer an environmentally friendly and energy-sustainable method for water purification. However, a comprehensive review on preparation and application of photocatalytic membranes is still lacking. Systematically comparing different photocatalytic membrane fabrication methods and revealing the underlying mechanisms of their respective applications are of particular interest.

Unraveling the Function Mechanism of N-Doped Carbon-Encapsulated NaV(PO) Cathode toward High-Performance Sodium-Ion Battery with Ultrahigh Cycling Stability.

The NASICON-type NaV(PO) (NVP) is recognized as a potential cathode material for Na-ion batteries (SIBs). Nevertheless, its inherent small electronic conductivity induces limited cycling stability and rate performance. Carbon coating, particularly N-doped carbon, has been identified as an effective strategy to address these challenges.

Cobalt Hexacyanoferrate Cathode with Stable Structure and Fast Kinetics for Aqueous Zinc-Ion Batteries.

Prussian blue analogues (PBAs) show great promise as cathode candidates for aqueous zinc-ion batteries thanks to their high operating voltage, open-framework structure, and low cost. However, suffering from numerous vacancies and crystal water, the electrochemical performance of PBAs remains unsatisfactory, with limited capacity and poor cycle life. Here, a simple coprecipitation method is shown to synthesize well-crystallized cobalt hexacyanoferrate (CoHCF) with a small amount of water and high specific surface area.

Regulable crack patterns for the fabrication of high-performance transparent EMI shielding windows.

Crack pattern-based metal grid film is an ideal candidate material for transparent electromagnetic interference shielding optical windows. However, achieving crack patterns with narrow grid spacing, small wire width, and high connectivity remains challenging. Herein, an aqueous acrylic colloidal dispersion was developed as a crack precursor for preparing crack patterns.

Regulation of Histone Acetylation Modification on Biosynthesis of Secondary Metabolites in Fungi.

The histone acetylation modification is a conservative post-translational epigenetic regulation in fungi. It includes acetylation and deacetylation at the lysine residues of histone, which are catalyzed by histone acetyltransferase (HAT) and deacetylase (HDAC), respectively. The histone acetylation modification plays crucial roles in fungal growth and development, environmental stress response, secondary metabolite (SM) biosynthesis, and pathogenicity.

Organocatalytic enantioselective synthesis of double S-shaped quadruple helicene-like molecules.

Helicene-shaped molecules are compelling chemical structures with unique twisted helical chirality and remarkable properties. Although progress occurs in the catalytic asymmetric synthesis of helicene (-like) molecules, the enantioselective synthesis of multiple helicenes, especially four or higher helicity, is still challenging and has yet to be achieved. Herein, we report an organocatalytic [4 + 2] cycloadditions to achieve double S-shaped quadruple helicene-like molecules with high enantioselectivity (up to 96% e.

Clinical Characteristics and Risk Factors of Patients with Lung Cancer Complicated with Pulmonary Embolism: A Case Control Study.

Purpose: The purpose of this study was to investigate the clinical characteristics and risk factors for patients with lung cancer complicated by pulmonary embolism and to provide a reference for the early clinical identification of these patients.

Methods: Eighty patients with lung cancer complicated with pulmonary embolism who were treated at Bethune Hospital of Shanxi from October 2018 to October 2025 were compared with 80 patients with lung cancer without pulmonary embolism. The clinical data of the two groups of patients were collected and analysed.

Hydrate formation in porous media with upward-migrating methane and its implications for the evolution of deep-sea cold seep ecosystems.

Methane leaking from the deep seabed is a primary source of carbon and energy for various microorganisms, sustaining the evolution and productivity of cold seep ecosystems. However, the dynamics of methane hydrate formation under methane seepage conditions and potential impacts on the evolution of cold seep ecosystems remain unclear. This study investigated the dynamic formation characteristics of gas hydrates within cold seep sediments by simulating the methane leakage process.

Perioperative ultrasound screening of lower extremity veins is effective in the prevention of fatal pulmonary embolism in orthopedic patients.

Patients at high risk of deep vein thrombosis are recommended to undergo lower-extremity ultrasonography to screen for pulmonary embolism (PE); however, there are few reports on whether this can effectively reduce the occurrence of fatal pulmonary embolism (FPE). This study aimed to assess the risk factors associated with PE and to investigate whether perioperative ultrasound screening of lower extremity veins in orthopedic patients can effectively reduce the incidence of FPE. We enrolled 137 patients with PE who underwent orthopedic surgery between 2013 and 2020.

Comprehensive Genomic and Proteomic Analysis Identifies Effectors of f. sp. .

wilt in eggplant caused by f. sp. is a major devastating soil-borne disease on a worldwide scale.

Platinum nanoparticles wrapped in carbon-dot-films as oxygen reduction reaction catalysts prepared by solution plasma sputtering.

Fuel cells have become increasingly important in recent years because of their high energy efficiency and low environmental impact. However, key challenges remain in the widespread adoption of fuel-cell vehicles, including reducing Pt usage in catalysts and improving their durability. In this study, a high-performance Pt@carbon-dot-film core-shell catalyst was successfully synthesized using a nonequilibrium reaction field, , solution plasma (SP) process, by adjusting the electrolyte pH.

The genomic landscape of short tandem repeats in cattle.

Short tandem repeats (STRs) are abundant and have high mutation rates across cattle genomes; however, comprehensive exploration of cattle STRs is needed. Here, we constructed a comprehensive map of 467 553 polymorphic STRs (pSTRs) constructed from 423 cattle genomes representing 59 breeds worldwide. We observed that pSTRs in coding sequences and 5'UTRs (Untranslated Regions) were under strong selective constraints and exhibited a relatively low level of diversity.

Photoreforming of Plastic Waste to Sustainable Fuels and Chemicals: Waste to Energy.

The extensive accumulation of plastic waste has given rise to severe environmental pollution issues. Contemporary conventional recycling methods, such as incineration and landfilling, contribute significantly to pollutant emissions and carbon footprints, against the principles of sustainable development. Leveraging renewable solar energy to transform plastics into high-value chemicals and green fuels offers a more promising and sustainable approach to managing plastic waste resources.

CaCO-encircled hollow CuS nanovehicles to suppress cervical cancer through enhanced calcium overload-triggered mitochondria damage.

Cervical cancer stands is a formidable malignancy that poses a significant threat to women's health. Calcium overload, a minimally invasive tumor treatment, aims to accumulate an excessive concentration of Ca within mitochondria, triggering apoptosis. Copper sulfide (CuS) represents a photothermal mediator for tumor hyperthermia.

Tailoring Cathode-Electrolyte Interface for High-Power and Stable Lithium-Sulfur Batteries.

Global interest in lithium-sulfur batteries as one of the most promising energy storage technologies has been sparked by their low sulfur cathode cost, high gravimetric, volumetric energy densities, abundant resources, and environmental friendliness. However, their practical application is significantly impeded by several serious issues that arise at the cathode-electrolyte interface, such as interface structure degradation including the uneven deposition of LiS, unstable cathode-electrolyte interphase (CEI) layer and intermediate polysulfide shuttle effect. Thus, an optimized cathode-electrolyte interface along with optimized electrodes is required for overall improvement.