Jieduquyuziyin prescription attenuates the side effect of prednisone through regulating gut microbiota when in the combination with prednisone treat MRL/lpr mice.

Jieduquyuziyin prescription (JP) is an empirical formula used to treat systemic lupus erythematosus (SLE). While JP has been shown to have synergistic and attenuated effects when combined with glucocorticoids (GCs) for SLE treatment, the precise mechanism remains unclear. This study utilized MRL/lpr mice to demonstrate the synergistic and attenuated effects of JP when combined with prednisone.

High-resolution spatio-temporal estimation of street-level air pollution using mobile monitoring and machine learning.

High spatio-temporal resolution street-level air pollution (SLAP) estimation is essential for urban air quality management, yet traditional methods face significant challenges in capturing the detailed spatial and temporal variability of pollution. Methods relying on fixed monitoring networks provide limited spatial coverage, while those utilizing mobile monitoring campaigns, despite their flexibility, often suffer from data sparsity and temporal incompleteness. To address these limitations, we propose a Two-Step Machine Learning Gap-Filling Framework employing a Multi-task Graph-based XGBoost (MTGXGB) model to enhance SLAP resolution.

Crystal Structure of a Thioredoxin-like Ferredoxin Encoded Within a Cobalamin Biosynthetic Operon of Rhodobacter capsulatus.

Thioredoxin-like ferredoxin is a small homodimeric protein containing a [2Fe-2S] cluster in each monomer. It is only found in bacteria but its physiological function remains largely unknown. The cobalamin biosynthetic operon in the genome of the purple phototroph Rhodobacter capsulatus encodes a putative ferredoxin dubbed as CfrX.

Construction of a Subcutaneous Fat Transplantation Model Infected with Mycobacterium.

Introduction: Fat grafting procedures for body contouring and cosmetic reconstruction have received widespread attention.

Method: In recent years, there has been an increase in post-fat grafting infections caused by Mycobacterium abscessus (MA), and there is a lack of representative and standardized murine models of infection; therefore, there has been limited research on the treatment of post-fat grafting MA infections. To overcome this challenge, we constructed an MA infection model after fat grafting.

Evaluating the impact of different processing methods on the flavor characteristics of Gorgon Euryale seeds using electronic tongue, electronic nose, gas chromatography-mass spectrometry, and gas chromatography-ion mobility spectrometry.

This study evaluated the volatile organic compounds (VOCs) and taste properties of Gorgon Euryale seeds processed by five methods (steaming, boiling, microwaving, roasting, and stir-frying) using electronic tongue (E-tongue), electronic nose (E-nose), gas chromatography-mass spectrometry (GC-MS), and gas chromatography-ion mobility spectrometry (GC-IMS). A total of 44 and 40 VOCs were identified by GC-MS and GC-IMS, respectively. Pyrazines (2-ethyl-3,5-dimethylpyrazine, 2,3-diethyl-5-methylpyrazine) and furans (2-pentylfuran, 2-ethylfuran) played a major role in the baked aroma characteristics of roasted and stir-fried Gorgon Euryale seeds.

Effect of intermittent theta burst stimulation combined with acoustic startle priming motor training on upper limb motor function and neural plasticity in stroke individuals: study protocol for a randomised controlled proof-of-concept trial.

Introduction: Stroke is a major cause of acquired disability globally, yet the neural mechanisms driving motor recovery post-stroke remain elusive. Recent research has underscored the growing significance of subcortical pathways in neural plasticity and motor control. Among these, the cortico-reticulospinal tract (CRST) has gained attention in rehabilitation due to its unique ascending and descending structural features as well as its cellular properties which position it as an excellent candidate to compensate for inadequate motor control post-stroke.

Design, Synthesis and Anti-Inflammatory Evaluation of 3-Substituted 5-Amidobenzoate Derivatives as Novel P2Y Receptor Antagonists via Structure-Guided Molecular Hybridization.

The P2YR is activated by UDP and UDP glucose and is involved in many human inflammatory diseases. Based on the molecular docking analysis of currently reported P2YR antagonists and the crystallographic overlap study between PPTN and compound , a series of 3-substituted 5-amidobenzoate derivatives were designed, synthesized, and identified as promising P2YR antagonists. The optimal compound (methyl 3-(1-benzo[]imidazol-2-yl)-5-(2-(-tolyl) acetamido)benzoate, IC = 0.

Dihydrotanshinone I improves cardiac function by promoting lymphangiogenesis after myocardial ischemia-reperfusion injury.

Dihydrotanshinone I (DHT) is an active ingredient derived from Salvia miltiorrhiza. Previous studies have demonstrated that DHT can improve cardiac function in rats with myocardial ischemia-reperfusion injury (IR). However, the mechanism by which DHT improves myocardial injury in rats still requires further research.

Structural Basis for Monomer-Dimer Transition of Dri1 Upon Heme Binding.

Domain related to iron (DRI) contains approximately 90 residues and is involved in iron and heme metabolism. Recent discoveries have annotated Dri1, a DRI-only protein from the cyanobacterium Synechocystis, as a regulator of succinate dehydrogenase in a b-type heme-dependent manner or as a c-type heme oxygenase. Here, we report high-resolution structures of Dri1 in complex with b-type and c-type hemes, respectively.

A review of studies on constructing classification models to identify mental illness using brain effective connectivity.

Brain effective connectivity (EC) is a functional measurement that reflects the causal effects and topological relationships of neural activities. Recent research has increasingly focused on the classification for mental illnesses and healthy controls using brain EC; however, no comprehensive reviews have synthesized these studies. Therefore, the aim of this review is to thoroughly examine the existing literature on constructing diagnosis model for mental illnesses using brain EC.

Effects of 1-Deoxynojirimycin Extracts of Mulberry Leaves on Oxidative Stress and the Function of the Intestinal Tract in Broilers Induced by HO.

The poultry industry struggles with oxidative stress affecting gut health and productivity. This study examined using 1-Deoxynojirimycin (DNJ) extracts from mulberry leaves as an antioxidant in broilers feed to combat this issue. We divided 240 broilers, aged 16 days, into six groups, including a control and groups exposed to oxidative stress through HO injections, with different supplement levels of DNJ-E (40, 80, 120, and 160 mg/kg of the basal diet) lasting until the broilers reached 42 days old.

Single-laser excitation synergistic photo- and chemodynamic therapy system based on persistent luminescence nanoparticles.

Photodynamic and photothermal therapy (PDT and PTT) have been widely used in tumor treatment researches owe to its advantages of spatiotemporal controlliability and non-invasiveness. Combining two phototherapy strategies together and/or with chemodynamic therapy (CDT) could achieve better therapeutic efficiency, but the resulting inconvenient dual-laser irradiation and the potential skin toxicity limit its development. Moreover, the lack of tumor-specificity causes side-effects to normal tissues.

Crystal structure of l-threonine-O-3-phosphate decarboxylase CobC from Sinorhizobium meliloti involved in vitamin B biosynthesis.

Vitamin B is involved in many important biochemical reactions for humans, and its deficiency can lead to serious diseases. The industrial production of vitamin B is achieved through microbial fermentation. In this work, we determine the crystal structures of the l-threonine-O-3-phosphate (Thr-P) decarboxylase CobC from Sinorhizobium meliloti (SmCobC), an industrial vitamin B-producing bacterium, in apo form and in complex with a reaction intermediate.

Efficient Inhibition of Ice Recrystallization During Frozen Storage: Based on the Diffusional Suppression Effect of Silk Fibroin.

Effectively controlling ice recrystallization (IR) during the frozen storage of food remains highly challenging. Inspired by the structural characteristics of antifreeze proteins in nature, silk fibroin (SF) derived from silk fibers has been developed. Through dual validation using the "splat" assay and "sucrose sandwich" assay, the IR inhibition activity of SF at various concentrations was confirmed, revealing that its regular alternating hydrophilic/hydrophobic domains endow SF with the potential to inhibit the axial growth of single ice crystal and significantly reduce the average maximum crystal size by approximately 67%.

Local Synchronization for Delayed Complex Dynamical Networks via Self-Triggered Impulsive Control Involving Delays.

This article investigates the local synchronization for delayed complex dynamical networks (CDNs) under self-triggered impulsive control (STIC) approaches involving delays. With the help of Lyapunov-Razumikhin methods and comparison principle, some design criteria of STIC strategies ensuring local synchronization for delayed CDNs with delayed impulses are provided, and Zeno behavior can be avoided. Compared with the existing results on synchronization of CDNs under STIC, in this article, time delays in both continuous and discrete system dynamics are well considered.

Structural basis for RNA recognition by the C-terminal RRM domain of human RBM45.

RBM45 is an RNA-binding protein with roles in neural development by regulating RNA splicing. Its dysfunction and aggregation are associated with neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and frontotemporal lobar dementia (FTLD). RBM45 harbors three RRM domains that potentially bind RNA.

JAK2 inhibitor protects the septic heart through enhancing mitophagy in cardiomyocytes.

Sepsis-induced myocardial dysfunction (SIMD) is a severe complication in sepsis, manifested as myocardial systolic dysfunction, which is associated with poor prognosis and higher mortality. Mitophagy, a self-protective mechanism maintaining cellular homeostasis, plays an indispensable role in cardioprotection. This study aimed to unveil the cardioprotective effects of Baricitinib on LPS-induced myocardial dysfunction and its effect on mitophagy.

Inhibition of GRK2 ameliorates the pristane-induced mouse SLE model by suppressing plasma cells differentiation.

Systemic lupus erythematosus (SLE) is a multifaceted autoimmune disorder characterized by diverse clinical manifestations and organ damage. Despite its elusive etiology, dysregulated subsets and functions of B cells are pivotal in SLE pathogenesis. Peoniflorin-6'-O-benzene sulfonate (CP-25), an esterification modification of Paeoniflorin, exhibits potent anti-inflammatory and immunomodulatory properties in autoimmune diseases (AID).

Discovery of N-Substituted Acetamide Derivatives as Promising P2YR Antagonists Using Molecular Hybridization Based on Crystallographic Overlay.

P2Y receptor (P2YR) is activated by uridine 5'-diphosphate-glucose, which is involved in many human inflammatory diseases. Based on the molecular docking analysis of currently reported P2YR antagonists and the crystallographic overlap study between the reported P2YR antagonist compounds and , a series of N-substituted-acetamide derivatives were designed, synthesized, and identified as novel and potent P2YR antagonists. The most potent antagonist, compound (-(1-benzo[]imidazol-6-yl)-2-(4-bromophenoxy)acetamide, IC = 0.

Crystal structure of methyltransferase CbiL from Akkermansia muciniphila.

Akkermansia muciniphila is a mucin-degrading probiotic that colonizes the gastrointestinal tract. Genomic analysis identified a set of genes involved in the biosynthesis of corrin ring, including the cobalt factor II methyltransferase CbiL, in some phylogroups of A. muciniphila, implying a potential capacity for de novo synthesis of cobalamin.

Efficacy of Huoxue Qianyang Qutan Recipe on essential hypertension: A randomized, double-blind, placebo-controlled trial.

Background: Hypertension, a prevalent disease, is a significant risk factor for coronary heart disease. Huoxue Qianyang Qutan Recipe (HQQR), a traditional Chinese herbal remedy, has been used for treating hypertension over several years.

Objective: This study assesses HQQR's efficacy for controlling blood pressure among patients with hypertension related to blood stasis, yang hyperactivity and phlegm.