MPCD Index for Hepatocellular Carcinoma Patients Based on Mitochondrial Function and Cell Death Patterns.

Hepatocellular carcinoma (HCC) is a highly heterogeneous cancer with a poor prognosis. During the development of cancer cells, mitochondria influence various cell death patterns by regulating metabolic pathways such as oxidative phosphorylation. However, the relationship between mitochondrial function and cell death patterns in HCC remains unclear.

Multi-omics analysis of soil microbiota and metabolites in dryland wheat fields under different tillage methods.

No-tillage and subsoiling can improve soil aggregate structure and realize a synergistic effect of soil carbon and nitrogen retention compared with deep tillage. This study aimed to investigate the effects of different tillage methods on the microbiome and metabolites in wheat rhizosphere. Results indicated that no significant differences in the diversity of soil bacterial and fungal communities were observed among the tillage methods.

Lingguizhugan decoction ameliorates cognitive impairment in AD-like mice by influencing the microbiome-gut-brain axis mediated by SCFAs.

Background: Lingguizhugan (LGZG) decoction, an ancient Chinese herbal remedy originating from the Eastern Han Dynasty, consists of Poria cocos, Cinnamomi ramulus, Atractylodes macrocephala, and Glycyrrhiza, as described in the Golden Chamber Synopsis. It has a history spanning over 1600 years, in which it has been primarily used for the treatment of inflammation, injuries, and fluid retention; however, the potential of LGZG decoction to ameliorate Alzheimer's disease (AD) progression by modulating the gut-brain axis through attenuation of gut microbiota and their metabolites remains unknown.

Purpose: To examine the in vivo anti-AD effects and mechanism of LGZG decoction in alleviating AD cognitive impairment.

A nation-wide study for the occurrence of PPD antioxidants and 6PPD-quinone in road dusts of China.

N,N'-substituted p-phenylenediamines (PPDs) are widely used antioxidants in rubber tires, which could be released and accumulated in road dusts with rubber tires wear. As ozonation product of N-(1,3-dimethylbutyl)-N'-phenyl-p-phenylenediamine (6PPD), 6PPD-quinone (6PPD-Q) exhibited higher toxicity to coho salmon. However, studies on their environmental behaviors are still limited.

[New pretreatment method for detecting petroleum hydrocarbons in soil: silica-gel dehydration and cyclohexane extraction].

Oil is a primary source of energy worldwide. However, the use of oil produces large amounts of pollutants, which are detrimental to the environment. The presence of petroleum hydrocarbons in soil is a critical marker of environmental pollution and safety.

Internal exposure of Chinese children from a typical coastal city to bisphenols and possible association with thyroid hormone levels.

Bisphenols (BPs) are widely used in consumer products, and human exposure to BPs is nearly ubiquitous. However, human biomonitoring data are scarce, especially for children. In this study, we quantified eight BPs in the serum of 345 children from a typical coastal city in China.

[Evaluation of thermal adsorption and desorption properties of dioxins on 11 adsorbents].

The key to the online thermal capture of dioxins is the appropriate choice of adsorbent for efficient capture at low temperatures and rapid desorption at high temperatures. Efficient adsorbents can allow for capture and separation during the online monitoring of dioxins or during offline dioxin tests. In this study, 1,2,3,4-tetrachlorodibenzo--dioxin (1,2,3,4-TCDD) and pentachlorodibenzofuran (1,2,3,8,9-PCDF) were used as model compounds for dioxin monomers, and an electron capture detector (ECD) was used to detect the dioxin signals.

The dose effect of dansyl chloride on the derivative products of bisphenols and its application for the determination of bisphenols in human serum by high-performance liquid chromatography-tandem mass spectrometry.

Human exposure to bisphenols has rarely been reported. The most important challenges in this regard are the sensitivity and accuracy of the analytical methods employed. Dansyl chloride derivatization prior to high-performance liquid chromatography-tandem mass spectrometry has been prevalently employed to improve sensitivity.

Monitoring gas- and particulate-phase short-chain polychlorinated paraffins in the urban air of Dalian by a self-developed passive sampler.

The concentration of short-chain polychlorinated paraffins (SCCPs) in the urban air of Dalian, China was monitored from September 2016 to August 2017 with a self-developed passive sampler (PAS1) and an active high-volume sampler, simultaneously. PAS1 successfully collected the entire target SCCPs in the ambient air. Air SCCPs sampled by PAS1 were found be in the linear uptake stage during 181 days of sampling.

Hyperbranched mixed-mode anion-exchange polymeric sorbent for highly selective extraction of nine acidic non-steroidal anti-inflammatory drugs from human urine.

This paper describes the poly(divinylbenzene) (PDVB) supported synthesis of quaternized hyperbranched macromolecules (QHMs) and its use as a highly selective, high-capacity mixed-mode anion-exchange (MAX) sorbent. In detail, the aminated PDVB support was firstly synthesized by copolymerization of divinylbenzene and 2-(diethylamino)ethyl methacrylate via Pickering emulsion polymerization. The QHMs were then grafted on PDVB by a divergent synthesis involving consecutive reactions of resorcinol diglycidyl ether with methylamine (N, N-dimethylethanolamine for terminal epoxides), which brought in a high density of quaternary ammonium functionalities.

Levels and gas-particle partitioning of hexabromocyclododecanes in the urban air of Dalian, China.

The concentrations of α-, β-, and γ-hexabromocyclododecane diastereomers (HBCDs) in gas phase and particulate phase of Dalian urban air, China, were monitored from September 2016 to August 2017 with high-volume active sampler. The total concentration of ∑HBCDs (gas phase + particulate phase) ranged from 15.47 to 43.

Ammonium hydroxide enhancing electrospray response and boosting sensitivity of bisphenol A and its analogs.

As one of the most important analytical techniques for accurate quantification of bisphenol compounds, liquid chromatography coupled to online electrospray ionization (ESI) tandem mass spectrometry exhibits relative weak ESI response in negative mode, which greatly reduces their sensitivity and limits their detection at trace levels, especially in complex samples such as blood. Based on the facilitated deprotonation of bisphenol molecule under alkaline condition, post-column injection of ammonium hydroxide (NH·HO) to mass spectrometer was explored to enhance the ionization efficiency of BPA and its eight analogs and improve their analytical sensitivity. Parameters effecting response intensity and stability were investigated, including mass concentration and flow rate of NH·HO.

Multiresidue determination and potential risks of emerging pesticides in aquatic products from Northeast China by LC-MS/MS.

A simple method for determining 33 pesticides with a wide polarity range (logK 0.6-4.5) in aquatic products was developed based on LC-MS/MS.

Hexabromocyclododecane and tetrabromobisphenol A in sediments and paddy soils from Liaohe River Basin, China: Levels, distribution and mass inventory.

Hexabromocyclododecane (HBCD) and tetrabromobisphenol A (TBBPA) are two kinds of brominated flame retardants and widely present in the environment and biota. The levels, spatial distributions and mass inventories of HBCD and TBBPA were investigated in sediments and paddy soils from the Liaohe River Basin in northeast China. The concentrations of ΣHBCD and TBBPA were in the range of not detected (nd) to 4.

[Non-target screening of organic pollutants in sediments and sludges using gas chromatography-mass spectrometry and automated mass spectral deconvolution].

A screening method in the combination of ultrasonic extraction, gas chromatography-mass spectrometry detection and automated mass spectrometry deconvolution technique was developed for non-target screening of non-polar and weak polar pollutants in sediments and sludges. The samples were extracted by ultrasonication for 20 min using dichloromethane for three times. The extraction solutions were cleaned-up by gel permeation chromatography and a silica gel column, and then 3 g of copper powder was used to remove the sulfur by ultrasonication for 10 min.

Dendrimer-functionalized mesoporous silica as a reversed-phase/anion-exchange mixed-mode sorbent for solid phase extraction of acid drugs in human urine.

A new dendrimer-functionalized mesoporous silica material based on large-pore 3D cubic Korea Advanced Institute of Science and Technology-6 (KIT-6) was synthesized by the growing of dendritic branches inside the mesopores of aminopropyl functionalized KIT-6. Detailed physical characterizations using transmission electron microscopy, nitrogen adsorption-desorption measurements, Fourier transform infrared (FTIR) spectroscopy, and elemental analysis reveal that the multifunctional dendrimers have been grown successfully within the confined spaces of mesopores. Although the 3D ordered mesoporous architecture of KIT-6 was well preserved, there was a significant and continuous decrease in pore size, specific surface area (SBET) and pore volume when increasing dendrimer generation up to six.

New reversed-phase/anion-exchange/hydrophilic interaction mixed-mode stationary phase based on dendritic polymer-modified porous silica.

A novel dendritic polymer-modified silica (DPS) stationary phase was prepared by a divergent synthesis scheme starting from propylamine on silica by consecutive amine-epoxy reactions with 1,4-butanedioldiglycidyl ether and aniline. Both elemental analysis and infrared spectra data shows the successful growth of dendritic polymer on silica particles. The carbon and nitrogen contents increased with an increasing number of reaction cycles and achieved 25.

Comprehensive screening and priority ranking of volatile organic compounds in Daliao River, China.

An analytical strategy for comprehensive screening of target and non-target volatile organic compounds (VOCs) in surface water was developed, and it was applied to the analysis of VOCs in water samples from Daliao River. The target VOCs were quantified using purge and trap-gas chromatography-mass spectrometry (P&T-GC/MS). Among 20 water samples, 34 VOCs were detected at least once.

Bioaccumulation and trophic transfer of polybrominated diphenyl ethers (PBDEs) in a marine food web from Liaodong Bay, North China.

The concentrations of 21 polybrominated diphenyl ethers (PBDEs) congeners were analyzed in organisms within a marine food web collected from the Liaodong Bay, North China. The total concentrations of PBDEs in all samples ranged from 0.87 to 91.

[Simultaneous determination of benzotriazoles and benzothiazole in surface water by solid phase extraction and high performance liquid chromatography].

A simple method based on solid phase extraction (SPE) coupled with high performance liquid chromatography (HPLC) was developed for the determination of 1H-benzotriazole (BT), 5-methyl-1H-benzotriazole (5-M-BT), 5,6-dimethyl-1H-benzotriazole (5,6-DM-BT), 5-chloro-1H-benzotriazole (5-Cl-BT) and benzothiazole (BTH) in surface water. The extraction and clean-up of the surface water sample were performed on an HLB solid-phase extraction cartridge. The separation of the five compounds was achieved on a ZORBAX SB-C18 column (250 mm x 4.

[Novel method for the identification of illegal cooking oil (2) : determination of special odd-chain fatty acids by multidimensional gas chromatography-mass spectrometry].

Endogenesis referents from illegal cooking oil (ICO), namely, 13-methyl-tetradecanoic acid and undecanoic acid have been confirmed via non-target and target screening of fatty acids. The former is mainly originated from animal oil, and the later from heated vegetable oil. Based on the retention interactions between gas chromatographic columns with different polarities and alkyl acids, the alkyl acids with various carbon chain or their isomers with the same carbon chains can be separated effectively using multidimensional gas chromatographymass spectrometry.

[Analysis of pollution levels of 16 antibiotics in the river water of Daliao River water system].

The detection of the pollution level of antibiotics in Daliao River system is a meaningful work. Sixteen antibiotics (6 sulfonamides, 5 fluoroquinolones, 3 tetracyclines and 2 chloramphenicols) were simultaneously quantified with solid-phase extraction (SPE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). In the SPE procedure, methanol and 2% (v/v) ammonia/methanol were used as the elution solvents in sequence to reduce the elution volume and improve the recovery.