Featuring few essential Raman spectroscopic signatures between heterogeneous cells.

Here we demonstrate it is instructive to quantify cell Raman spectroscopy by sparse regularization. To be able to extract the specific spectral differences in a heterogeneous cell system with great spectroscopic similarities derived from many common biomolecular components, the maximum information entropy probability was proposed and exemplified by identifying normal lymphocytes from leukemia cells. The essential spectroscopic features were observed to locate at three Raman peaks whose spectral signatures were commensurate.

[Emission Characteristics and Emission Factors of Volatile Organic Compounds from E-waste Dismantling and Recycling Processes].

A large number of volatile organic compounds (VOCs) are emitted from the high temperature treatment process in the dismantling and recycling procedure of e-wastes, which has a significant impact on the surrounding environment and human health. In this study, an e-waste dismantling and recycling yard was selected to measure the VOCs concentrations and compositions in the exhaust of treatment facilities of heating baking board, plastic granulation, wet extraction, and pyrometallurgical workshops, and the emission characteristics of VOCs and emission factors for total VOCs from different production processes were investigated. The results showed that there were significant differences in total VOCs emission concentrations among different production processes.

Mixed Ionically/Electronically Conductive Double-Phase Interface Enhanced Solid-State Charge Transfer for a High-Performance All-Solid-State Li-S Battery.

An all-solid-state lithium-sulfur battery (ASSLSB) is a promising candidate for post-Li-ion battery technologies with high energy densities and good safety performance. However, the intrinsic insulating nature of sulfur requires triple-phase contact with an ionic conductor and an electronic conductor for electrochemical reactions, which decreases the amount of active surface and lowers the charge-transfer efficiency. In this work, a double-phase interface constructed from a mixed ionic/electronic conductor is proposed to enhance the solid-state electrochemical reaction of sulfur.

Progressive muscle weakness and amyotrophy during pregnancy as the first manifestation of systemic lupus erythematosus: A case report and review of literature.

Background: Systemic lupus erythematosus is a common autoimmune disease involving multiple systems. Clinical involvement of the central and peripheral nervous systems is not unusual, but peripheral neuropathy in systemic lupus erythematosus with chronic inflammatory demyelinating polyneuropathy is uncommon. Our study aimed to illustrate the clinical features, diagnosis, and treatment of systemic lupus erythematosus combined with chronic inflammatory demyelinating polyneuropathy, and to aid in the identification of peripheral neuropathy in systemic lupus erythematosus.

Pharmacological regulation of tissue fibrosis by targeting the mechanical contraction of myofibroblasts.

Fibrosis can occur in almost all tissues and organs and affects normal physiological function, which may have serious consequences, such as organ failure. However, there are currently no effective, broad-spectrum drugs suitable for clinical application. Revealing the process of fibrosis is an important prerequisite for the development of new therapeutic targets and drugs.

Evolution of Hepatitis B Virus (HBV) Serologic Markers Among Antiretroviral-Naive Young People Living With Human Immunodeficiency Virus Who Had Undergone Neonatal HBV Vaccination and Initiated Antiretroviral Therapy.

Background: With initiation of antiretroviral therapy (ART) containing nucleos(t)ide reverse-transcriptase inhibitors (NRTIs) with anti-hepatitis B virus (HBV) activity, the evolution of HBV serologic markers among people living with human immunodeficiency virus (PLWH) who were born in the era of nationwide neonatal HBV vaccination is rarely investigated.

Methods: This retrospective cohort study evaluated the changes of HBV serologic markers (hepatitis B surface antigen [HBsAg], antibody to hepatitis B surface antigen [anti-HBs], and antibody to hepatitis B core antigen [anti-HBc]) of PLWH who had undergone neonatal HBV vaccination. Clinical characteristics were analyzed and the incidences of evolution of HBV serologic markers were estimated.

Bioinspired 2D Isotropically Fatigue-Resistant Hydrogels.

Engineering conventional hydrogels with muscle-like anisotropic structures can efficiently increase the fatigue threshold over 1000 J m along the alignment direction; however, the fatigue threshold perpendicular to the alignment is still as low as ≈100-300 J m , making them nonsuitable for those scenarios where isotropic properties are desired. Here, inspired by the distinct structure-properties relationship of heart valves, a simple yet general strategy to engineer conventional hydrogels with unprecedented yet isotropic fatigue resistance, with a record-high fatigue threshold over 1,500 J m along two arbitrary in-plane directions is reported. The two-step process involves the formation of preferentially aligned lamellar micro/nanostructures through a bidirectional freeze-casting process, followed by compression annealing, synergistically contributing to extraordinary resistance to fatigue crack propagation.

Endoscopic Ultrasound-Guided Drainage in the Management of Postoperative Pancreatic Fistula After Partial Pancreatectomy.

Background: Postoperative pancreatic fistula (POPF) is the most frequent and harmful complication following pancreatic surgery. Traditional management includes conservative treatment, percutaneous drainage (PD), and reoperation. The objective of the present study was to evaluate the safety and effectiveness of EUS (Endoscopic ultrasound)-guided drainage by using nasocystic tubes combined with single or 2 stents for POPF.

[Pharmacokinetics and tissue distribution characteristics of the novel photosensitizer 3-(4-methoxyphenyl)-15-aspartyl-chlorin e6].

Photodynamic therapy (PDT) has garnered immense research interest. PDT can directly kill the cells via a combination of photosensitizer, light, and molecular oxygen. It has emerged as a promising therapeutic option for cancer treatment owing to its advantages such as minimized systemic toxicity, minimal invasiveness, high therapeutic efficacy, and potential for developing antitumor immunity.

[LPS stimulating neutrophils firmly adhered to ICAM-1 to form extracellular traps depends on integrin Mac-1 and cytoskeletal proteins].

Neutrophil extracellular traps (NETs) play an important role in the formation of immunothrombosis. However, how vascular endothelial cells mediate the formation of NETs has not been fully understood. We stimulated neutrophils firmly attached on the endothelial cell surface intercellular adhesion molecule-1 (ICAM-1) with lipopolysaccharide (LPS) or phorbol-12-myristate-13-acetate (PMA) for 4 h, then labeled NETs-DNA with Sytox green dye and the formation of NETs was observed by fluorescent microscopy.

Arginine Regulates TOR Signaling Pathway through SLC38A9 in Abalone .

Arginine plays an important role in the regulation of the target of the rapamycin (TOR) signaling pathway, and Solute Carrier Family 38 Member 9 (SLC38A9) was identified to participate in the amino acid-dependent activation of TOR in humans. However, the regulations of arginine on the TOR signaling pathway in abalone are still unclear. In this study, of abalone was cloned, and the was knocked down and overexpressed to explore its function in the regulation of the TOR signaling pathway.

Shigella evades pyroptosis by arginine ADP-riboxanation of caspase-11.

Mouse caspase-11 and human caspase-4 and caspase-5 recognize cytosolic lipopolysaccharide (LPS) to induce pyroptosis by cleaving the pore-forming protein GSDMD. This non-canonical inflammasome defends against Gram-negative bacteria. Shigella flexneri, which causes bacillary dysentery, lives freely within the host cytosol where these caspases reside.

An ultrasound-driven immune-boosting molecular machine for systemic tumor suppression.

Exploring facile and effective therapeutic modalities for synergistically controlling primary tumor and metastasis remains a pressing clinical need. Sonodynamic therapy (SDT) offers the possibility of noninvasively eradicating local solid tumors, but lacks antimetastatic activity because of its limited ability in generating systemic antitumor effect. Here, we exploited a previously unidentified ultrasound-driven “molecular machine,” DYSP-C34 (C34 for short), with multiple attractive features, emerging from preferential tumor accumulation, potent ultrasound-triggered cytotoxicity, and intrinsic immune-boosting capacity.

NELL1 augments osteogenesis and inhibits inflammation of human periodontal ligament stem cells induced by BMP9.

Background: Periodontitis could lead to periodontal destruction such as the loss of alveolar bone. The issue that how to achieve the regeneration of alveolar bone and periodontal tissues under the inflammatory environment needs to be solved urgently. Bone morphogenetic protein 9 (BMP9) is one of the most potent osteoinductive BMPs and induces osteogenic differentiation of mesenchymal stem cells.

Label-Free Colorimetric Method for Detection of by Trimming the G-Quadruplex DNAzyme with CRISPR/Cas12a.

(), which may cause gastrointestinal disorders in humans, is a pathogen commonly found in seafood. There are many methods for detecting , yet they have some shortcomings, such as high cost, labor-intensiveness, and complicated operation, which are impractical for resource-limited settings. Herein, we present a sequence-specific, label-free, and colorimetric method for visual detection of .

Correlation and Difference Between Raman Spectral Characteristic and Feature Evaluation for Leukocytes and Tumor Cells.

Tumor detection supported by Raman spectroscopy is becoming increasingly popular, yet the relevance of spectral variation and feature selection retains unclear. Here we determined the correlation and difference between spectral characteristic and feature evaluation for leukocytes and tumor cells. Some peaks were found to show noticeable spectral differences, and their intensity distributions were investigated, finding using log-normal distribution to describe Raman intensity pattern may be more appropriate.

Epigenetic Regulation of β Cell Identity and Dysfunction.

β cell dysfunction and failure are driving forces of type 2 diabetes mellitus (T2DM) pathogenesis. Investigating the underlying mechanisms of β cell dysfunction may provide novel targets for the development of next generation therapy for T2DM. Epigenetics is the study of gene expression changes that do not involve DNA sequence changes, including DNA methylation, histone modification, and non-coding RNAs.

The Potential Roles of T Cells in Periapical Lesions.

Introduction: Periapical lesions are inflammatory diseases mainly caused by microbial infection from the root canal system, affecting the integrity of alveolar bone, periapical cementum, and periodontal ligament. The invasion of pathogenic microorganisms activates local inflammation and host immune response, especially the recruitment and differentiation of T cells. Many studies have discussed the fundamental roles of T cell-related immunological regulation and the possible clinical significance of cytokine disorders in periapical lesions.

Potential relationship between clinical symptoms and the root canal microbiomes of root filled teeth based on the next-generation sequencing.

Aim: To determine the microorganism in root canal systems of root filled teeth with periapical disease and their relationship with clinical symptoms using next-generation sequencing.

Methodology: The roots of 10 extracted teeth were collected from 10 patients who presented with post-treatment apical periodontitis (PTAP; six with symptoms and four without symptoms). Each root was divided horizontally into two parts (apical and coronal segments) and cryo-pulverized.

Construction of hierarchical-porous metal-organic frameworks through esterification reaction for efficient catalysis.

A solvent-assisted strategy was proposed by controlling the coordination equilibrium to fabricate hierarchical-porous metal-organic frameworks (HP-MOFs). The obtained HP-MOFs showed remarkable enhancement in catalytic efficiency in Lewis acid catalysis resulting from the joint efforts of the hierarchical pores and the exposed metal clusters.

Ascorbic Acid Regulates the Immunity, Anti-Oxidation and Apoptosis in Abalone Ino.

The present study was conducted to investigate the roles of ascorbic acid (AA) in immune response, anti-oxidation and apoptosis in abalone ( Ino). Seven semi-purified diets with graded levels of AA (0, 50, 100, 200, 500, 1000 and 5000 mg/kg) were fed to abalone (initial weight: 12.01 ± 0.

Highly Sensitive Capacitive Pressure Sensors over a Wide Pressure Range Enabled by the Hybrid Responses of a Highly Porous Nanocomposite.

Past research aimed at increasing the sensitivity of capacitive pressure sensors has mostly focused on developing dielectric layers with surface/porous structures or higher dielectric constants. However, such strategies have only been effective in improving sensitivities at low pressure ranges (e.g.

A Pan-Cancer Analysis of the Prognostic Value and Expression of Adenylate Cyclase 7 (ADCY7) in Human Tumors.

Background: The role of adenylate cyclase 7 (ADCY7) in cancer is still unclear. This study analyzed the interrelationship between the expression and immune function of ADCY7.

Methods: ADCY7 expression in multiple human cancers was analyzed using the databases of Genotype-Tissue Expression Project (GTEx), Cancer Cell Line Encyclopedia (CCLE), and The Cancer Genome Atlas (TCGA).

MicroRNA as an Important Target for Anticancer Drug Development.

Cancer has become the second greatest cause of death worldwide. Although there are several different classes of anticancer drugs that are available in clinic, some tough issues like side-effects and low efficacy still need to dissolve. Therefore, there remains an urgent need to discover and develop more effective anticancer drugs.