Coacervate vesicles assembled by liquid-liquid phase separation improve delivery of biopharmaceuticals.

Vesicles play critical roles in cellular materials storage and signal transportation, even in the formation of organelles and cells. Natural vesicles are composed of a lipid layer that forms a membrane for the enclosure of substances inside. Here we report a coacervate vesicle formed by the liquid-liquid phase separation of cholesterol-modified DNA and histones.

Lysoglycerophospholipid metabolism alterations associated with ambient fine particulate matter exposure: Insights into the pro-atherosclerotic effects.

The biological pathways connecting ambient fine particulate matter (PM)-induced initial adverse effects to the development of atherosclerotic cardiovascular diseases are not fully understood. We hypothesize that lysoglycerophospholipids (LysoGPLs) are pivotal mediators of atherosclerosis induced by exposure to PM. This study investigated the changes of LysoGPLs in response to PM exposure and the mediation role of LysoGPLs in the pro-atherosclerotic effects of PM exposure.

A small RNA effector conserved in herbivore insects suppresses host plant defense by cross-kingdom gene silencing.

Herbivore insects deploy salivary effectors to manipulate the defense of their host plants. However, it remains unclear whether small RNAs from insects function as effectors in regulating plant-insect interactions. Here, we report that a microRNA (miR29-b) found in the saliva of phloem-feeding whitefly (Bemisa tabaci) can transfer into the host plant phloem during feeding and fine-tune the defense response of tobacco (Nicotiana tabacum).

-Enriched Eicosatrienoic Acid Regulates Glucose Homeostasis by Promoting GLP-1 Secretion to Protect Intestinal Barrier Integrity.

is a well-known probiotic with beneficial effects, such as anti-insulin resistance, anti-inflammatory, and improvement of the intestinal barrier. However, the underlying mechanisms remain unclear. Here, we found that gavage of improved the intestinal barrier and glucose homeostasis in HFD-fed mice.

pH-regulated CQDs@Eu/GMP ICP sensor array and its fingerprinting on 96-well plates: Toward point-of-use/specific identification and quantitation of six tetracyclines in animal farm wastewater, milks and milk-derivative products.

Herein, a "lab-on-an-AIE@Ln/ICP" sensor array was constructed by employing aggregation-induced emission carbon quantum dots (AIE-CQDs) as the guest and Eu/GMP ICP as the host. Based on the antenna effect (AE) and reductive photo-induced electron transfer (r-PET) between CQDs@Eu/GMP ICPs and tetracyclines (TCs), the as-constructed sensor produced satisfactorily dual-emitting fluorescence. By combining pH regulation with principal component analysis (PCA), the underlying fingerprinting patterns realized the specific identification and quantitation of six TCs in animal farm wastewater, milks and milk-derivative products.

Two novel triazine-based quaternary ammonium salt Gemini surfactants as potential corrosion inhibitors for carbon steel in a sulfate-reducing bacteria solution: Experimental and theoretical studies.

In this paper, two triazine ring-containing quaternary ammonium salt Gemini surfactants (C-2-C and C-2-C) were synthesized. The minimum inhibitory concentrations against sulfate-reducing bacteria (SRB) of C-2-C, C-2-C and dodecyl dimethyl benzyl ammonium chloride (1227) were determined using the double-dilution method. The performance of C-2-C and C-2-C in inhibiting carbon steel corrosion in the presence of SRB was examined, with 1227 serving as a control sample.

High fluorescence quantum yield of methionine-doped carbon quantum dots for achieving rapid assay of tetracyclines in foodstuffs.

The trace-level detection of tetracyclines (TCs) in food products is essential to ensure food safety and public health. Herein, we prepared the methionine-doped carbon quantum dots (Met-CQDs) using citric acid as the precursor. Met-CQDs exhibited a Gaussian unimodal peak centered at 440 nm in the fluorescent excitation spectrum, along with a remarkable greenish-blue emission and a fluorescent quantum yield of 33.

Nicotine-Induced Transient Activation of Monocytes Facilitates Immunosuppressive Macrophage Polarization that Restrains T Helper 17 Cell Expansion.

Macrophages in smoking environment exhibit a distinct immunosuppressive phenotype, but the mechanisms that allow nicotine to "educate" macrophages are incompletely understood. Here, we identified that nicotine transiently activates and subsequently deactivates monocytes, leading to reduced anti-infective capability of macrophages. This deactivation results in a suppression of IL-17-producing cell expansion through decreased IL-1β production.

The SA-WRKY70-PR-Callose Axis Mediates Plant Defense Against Whitefly Eggs.

The molecular mechanisms of plant responses to phytophagous insect eggs are poorly understood, despite their importance in insect-plant interactions. This study investigates the plant defense mechanisms triggered by the eggs of whitefly , a globally significant agricultural pest. A transcriptome comparison of tobacco plants with and without eggs revealed that whitefly eggs may activate the response of defense-related genes, including those involved in the salicylic acid (SA) signaling pathway.

Using salicylic acid-responsive promoters to drive the expression of jasmonic acid-regulated genes enhances plant resistance to whiteflies.

Background: Jasmonic acid (JA) is an important phytohormone used to defend against herbivores, but it does not respond to whitefly feeding. Conversely, another phytohormone, salicylic acid (SA), is induced when plants are fed upon by whiteflies. JA has a better anti-whitefly effect than SA; however, there is limited research on how to effectively improve plant resistance by utilizing the different responses of these phytohormones to whitefly feeding.

The Effect of Different Doses of Ropivacaine for Caudal Block in School-aged Children Undergoing Shang Ring Circumcision.

Purpose: The present study aimed to evaluate the effects of three different doses of ropivacaine in Shang Ring circumcision in school-aged children.

Design: This is a prospective, randomized, controlled study.

Methods: A total of 148 American Society of Anesthesiologists I to II children were enrolled and randomly assigned into the R0.

Co-encapsulation of borage seed oil and peppermint oil blends within ultrasound-assisted soy protein isolate/purity gum ultra complex nanoparticles: Fabrication, structural interaction mechanisms, and in vitro digestion studies.

This study aimed at co-encapsulating borage seed oil (BSO)- and peppermint oil (PO) blends in ultrasound-assisted complex nanoparticles stabilized by soy protein isolate (SPI) and purity gum ultra (PGU) in different ratios: SPI/PGU-1:0 (NP), 0:1 (NP), 1:1 (NP), 1:3 (NP), and 3:1 (NP). The BSO- and PO-loaded SPI/PGU complex nanoparticles (BP-loaded SPNPs) coded as NP (SPI-PGU-1:3) revealed a zeta potential of -33.27 mV, a PDI of 0.

Carbon Dots: A Versatile Platform for Cu Detection, Anti-Counterfeiting, and Bioimaging.

Carbon dots (CDs) have garnered extensive interest in basic physical chemistry as well as in biomedical applications due to their low cost, good biocompatibility, and great aqueous solubility. However, the synthesis of multi-functional carbon dots has always been a challenge for researchers. Here, we synthesized novel CDs with a high quantum yield of 28.

Metabolic and Molecular Insights Into Nicotiana benthamiana Trichome Exudates: An Ammunition Depot for Plant Resistance Against Insect Pests.

Nicotiana benthamiana, a widely acknowledged laboratory model plant for molecular studies, exhibits lethality to certain insect pests and can serve as a dead-end trap plant for pest control in the field. However, the underlying mechanism of N. benthamiana's resistance against insects remains unknown.

Attraction of Nicotiana benthamiana to Bemisia tabaci is related to a chemical signal in plant volatile, undecane.

Background: The whitefly Bemisia tabaci is one of the world's foremost agricultural pests. Recently, we found that a wild relative of tobacco (Nicotiana benthamiana) demonstrates remarkable attractiveness and nearly 100% lethality towards whiteflies. Therefore, it can act as a dead-end trap crop for whitefly control in the field.

Eicosatrienoic acid inhibits estradiol synthesis through the CD36/FOXO1/CYP19A1 signaling pathway to improve PCOS in mice.

Polycystic ovary syndrome (PCOS) is a common metabolic and endocrine disorder characterized by abnormal elevation in hormone levels, with currently lacking effective treatment options. N-3 polyunsaturated fatty acids (PUFA) have broad pharmacological activity and play a beneficial role in the development of PCOS. In this study, we observed that n-3 PUFA-eicosatrienoic acid (ETA) improves the estrous cycle and ovarian morphology in dehydroepiandrosterone (DHEA)-induced PCOS mice, particularly serum hormone levels.

Efficient reclamation of phosphorus from wetland biomass waste via liquid-recirculated hydrothermal carbonization and precipitation.

Hydrothermal carbonization (HTC) for the recovery of phosphorus (P) from biomass wastes has attracted considerable attention, while migration of P to the liquid phase greatly weakened P recovery efficiency and elevated the environmental risk. Therefore, a systematic scheme was proposed in this work to accomplish the complete reclamation of P from wetland plant (Ceratophyllum demersum) through coupling liquid-recirculated HTC mediated by HO or HSO with precipitation, and the migration and speciation of P during this process was determined by P K-edge X-ray absorption near edge structure, P nuclear magnetic resonance, and the modified sequential extraction. The P concentration in the liquid phase increased with the recirculation of HTC process water, and reached up to 550.

Coordination analysis and evaluation of population, water resources, economy, and ecosystem coupling in the Tuha region of China.

The non-coordination between the socio-economic systems and ecosystems of a region is a crucial obstacle to sustainable development. To reveal the relationships between complex urban systems and achieve the goal of sustainable and coordinated urban development, we constructed a coupling coordination degree model (CCDM) and coupling angle model (CAM) and analyzed the degree of coupling coordination and evolution process among the population, water resources, economy, and ecology (PWEE) system of the Tuha region for 2005-2020. The results indicated that: (1) During 2005-2020, the comprehensive development index (CDI) of the population, water resources and economy subsystems was 0.

Cortical and subcortical gray matter abnormalities in mild cognitive impairment.

Gray matter changes are thought to be closely related to cognitive decline in mild cognitive impairment (MCI) patients. The study aimed to explore cortical and subcortical structural alterations in MCI and their association with cognitive assessment. 24 MCI patients and 22 normal controls (NCs) were included.

Effects of sand substrate removal on the intestinal antioxidant and metabolism in Marsupenaeus japonicus.

M. japonicus is an important specie for factory farming, and factory farming requires an environment with sand at the bottom of the pond. However, the physiological responses as well as survival in the process of factory farming without laying sand are currently unknown.

A cyclic di-GMP-binding adaptor protein interacts with a N5-glutamine methyltransferase to regulate the pathogenesis in Xanthomonas citri subsp. citri.

The second messenger cyclic diguanylate monophosphate (c-di-GMP) regulates a wide range of bacterial behaviours through diverse mechanisms and binding receptors. Single-domain PilZ proteins, the most widespread and abundant known c-di-GMP receptors in bacteria, act as trans-acting adaptor proteins that enable c-di-GMP to control signalling pathways with high specificity. This study identifies a single-domain PilZ protein, XAC3402 (renamed N5MapZ), from the phytopathogen Xanthomonas citri subsp.

Elucidating the regulatory role of long non-coding RNAs in drought stress response during seed germination in leaf mustard.

Leaf mustard ( L. Czern & Coss), an important vegetable crop, experiences pronounced adversity due to seasonal drought stress, particularly at the seed germination stage. Although there is partial comprehension of drought-responsive genes, the role of long non-coding RNAs (lncRNAs) in adjusting mustard's drought stress response is largely unexplored.

MYB2 and MYB108 regulate lateral root development by interacting with LBD29 in Arabidopsis thaliana.

AUXIN RESPONSE FACTOR 7 (ARF7)-mediated auxin signaling plays a key role in lateral root (LR) development by regulating downstream LATERAL ORGAN BOUNDARIES DOMAIN (LBD) transcription factor genes, including LBD16, LBD18, and LBD29. LBD proteins are believed to regulate the transcription of downstream genes as homodimers or heterodimers. However, whether LBD29 forms dimers with other proteins to regulate LR development remains unknown.

Development of novel lysosome-related signatures and their potential target drugs based on bulk RNA-seq and scRNA-seq for diabetic foot ulcers.

Background: Diabetic foot ulcers (DFU) is the most serious complication of diabetes mellitus, which has become a global health problem due to its high morbidity and disability rates and the poor efficacy of conventional treatments. Thus, it is urgent to identify novel molecular targets to improve the prognosis and reduce disability rate in DFU patients.

Results: In the present study, bulk RNA-seq and scRNA-seq associated with DFU were downloaded from the GEO database.

Microwave-Assisted Pyrolysis-A New Way for the Sustainable Recycling and Upgrading of Plastic and Biomass: A Review.

The efficient utilization of organic solid waste resources can help reducing the consumption of conventional fossil fuels, mitigating environmental pollution, and achieving green sustainable development. Due to its dual nature of being both a resource and a source of pollution, it is crucial to implement suitable recycling technologies throughout the recycling and upgrading processes for plastics and biomass, which are organic solid wastes with complex mixture of components. The conventional pyrolysis and hydropyrolysis were summarized for recycling plastics and biomass into high-value fuels, chemicals, and materials.