Staphylococcus aureus blocks host autophagy through circSyk/miR-5106/Sik3 axis to promote progression of bone infection.

With the rapid increase in the number of implant operations, the incidence of bone infections has increased. Methicillin-resistant Staphylococcus aureus (S. aureus) and other emerging fully drug-resistant strains make the management of bone infections even more challenging.

Comparative study of robotic-assisted vs. laparoscopic surgery for colorectal cancer: a single-center experience.

Background: Colorectal cancer (CRC) surgeries are commonly performed using either robotic-assisted colorectal surgery (RACS) or laparoscopic colorectal surgery (LCS). This study aimed to compare clinical and surgical outcomes between RACS and LCS for CRC patients.

Methods: We included 225 patients from Tianjin Medical University Cancer Institute & Hospital (TJMUCH) between January 2021 and June 2024, divided into RACS (n=82) and LCS (n=143) groups.

Backbone resonance assignments of PhoCl, a photocleavable protein.

PhoCl is a photocleavable protein engineered from a green-to-red photoconvertible fluorescent protein by circular permutation, and has been used in various optogenetic applications including precise control of protein localization and activity in cells. Upon violet light illumination, PhoCl undergoes a β-elimination reaction to be cleaved at the chromophore, resulting in spontaneous dissociation into a large empty barrel and a small C-terminal peptide. However, the structural determinants and the mechanism of the PhoCl photocleavage remain elusive, hindering the further development of more robust photocleavable optogenetic tools.

Vitrification of 3D-MSCs encapsulated in GelMA hydrogel: Improved cryosurvival, reduced cryoprotectant concentration, and enhanced wound healing.

Compared to traditional 2D-cultured mesenchymal stem cells (MSCs), 3D-MSCs offer distinct advantages in disease treatment. However, large-scale culture of 3D-MSCs remains labor-intensive and time-consuming. Thus, developing cryopreservation method for 3D-MSCs is essential for clinical application.

Comprehensive discovery and functional characterization of the noncanonical proteome.

The systematic identification and functional characterization of noncanonical translation products, such as novel peptides, will facilitate the understanding of the human genome and provide new insights into cell biology. Here, we constructed a high-coverage peptide sequencing reference library with 11,668,944 open reading frames and employed an ultrafiltration tandem mass spectrometry assay to identify novel peptides. Through these methods, we discovered 8945 previously unannotated peptides from normal gastric tissues, gastric cancer tissues and cell lines, nearly half of which were derived from noncoding RNAs.

Temperature has an enhanced role in sediment NO and N fluxes in wider rivers.

Riverine NO and N fluxes, key components of the global nitrogen budget, are known to be influenced by river size (often represented by average river width), yet the specific mechanisms behind these effects remain unclear. This study examined how environmental and microbial factors influenced sediment NO and N fluxes across rivers with varying widths (2.8 to 2,000 m) in China.

The Characteristics, Sources, and Health Risks of Volatile Organic Compounds in an Industrial Area of Nanjing.

This study investigates the chemical complexity and toxicity of volatile organic compounds (VOCs) emitted from national petrochemical industrial parks and their effects on air quality in an industrial area of Nanjing, China. Field measurements were conducted from 1 December 2022, to 17 April 2023, focusing on VOC concentrations and speciations, diurnal variations, ozone formation potential (OFP), source identification, and associated health risks. The results revealed an average total VOC (TVOC) concentration of 15.

Thermal insulating cellulose/wood foam for passive radiant cooling.

Passive cooling permits thermal management of near-zero energy consumption and low CO emissions. Herein, cellulose/wood chip composite foam (CWF) with anisotropic porous structure was prepared via freeze-casting strategy. The CWF displayed an average reflectance of up to 95.

Conjugated Linoleic Acid (CLA) Mitigates High-Fat Diet (HFD)-Induced Mammary Gland Development Impairment of Pubertal Mice via Regulating CD36 Palmitoylation and Downstream JNK-ERK Pathway.

Conjugated linoleic acid (CLA) is known for antiobesity. However, the role of CLA in regulating high-fat diet (HFD)-impaired pubertal mammary gland development remains undefined. Here, pubertal female mice and HC11 cells were treated with HFD or palmitic acid (PA), supplemented with or without CLA, respectively.

Spirochrains A-D, Four Caged [5,6,5] Spirocyclic Amides from an Antarctic Fungus SCSIO 05702 with Anti-RSV Activities.

Spirochrains A-D, four undescribed spirocyclic amides, were obtained from an Antarctic fungus, SCSIO 05702. Their structures were elucidated through spectroscopic analysis and quantum calculations. Spirochrains A-D possess a rarely reported cage-like 5/6/5 fused spirocyclic amide scaffold.

Efficacy of Laparoscopic Parenchyma-sparing Hepatectomy Using Augmented Reality Navigation Combined With Fluorescence Imaging for Colorectal Liver Metastases: A Retrospective Cohort Study Using Inverse Probability Treatment Weighting Analysis.

Background: Laparoscopic parenchyma-sparing hepatectomy (PSH) is an effective treatment for colorectal liver metastases (CRLMs), but ensuring the safety and radicality of the procedure, particularly for deep-seated tumors, remains challenging. Surgical navigation technologies such as augmented reality navigation (ARN) and indocyanine green fluorescence imaging (ICG-FI) are increasingly utilized to guide surgery, yet their efficacy for CRLMs is unclear. This study aims to evaluate the short- and long-term outcomes of ARN combined with ICG-FI-guided (ARN-FI) laparoscopic PSH for CRLMs.

Intelligent management and legal regulation of enterprise green supply chain by fuzzy comprehensive evaluation.

This study aims to evaluate the risk levels of intelligent management in Green Supply Chain Management (GSCM) through the application of the Fuzzy Comprehensive Evaluation (FCE) model. It begins by outlining the structure of the green supply chain (GSC), encompassing various components such as suppliers, manufacturers, and distributors, while incorporating considerations of environmental and sustainability factors. Data is collected from Company Y through a questionnaire survey, and the FCE model is employed to assess the risk levels of intelligent management within GSCM.

Magnetic field dependence of - defects in hexagonal boron nitride.

The interface with spin defects in hexagonal boron nitride has recently become a promising platform and has shown great potential in a wide range of quantum technologies. Varieties of spin properties of - defects in hexagonal boron nitride (hBN) have been researched widely and deeply, like their structure and coherent control. However, little is known about the influence of off-axis magnetic fields on the coherence properties of - defects in hBN.

Identification of a novel anti-ROR1 nanobody through phage display and its biochemical characterization.

In this study, we aimed to develop nanobodies targeting receptor tyrosine kinase-like orphan receptor 1 (ROR1) for cancer diagnosis and therapy. We immunized alpacas with ROR1, extracted RNA from their blood, and converted it to complementary DNA (cDNA) to amplify the VHH (variable domain of heavy-chain antibodies) sequence. This sequence was used to construct a phage library with a capacity of 8 ×10.

Knockdown of VEGF-B improves HFD-induced insulin resistance by enhancing glucose uptake in vascular endothelial cells via the PI3K/Akt pathway.

Vascular endothelial growth factor B (VEGF-B) has been suggested to play a crucial role in regulating whole-body glucose homeostasis. However, the involved mechanisms are not fully understood. This study aimed to elucidate the regulatory effects and mechanisms of VEGF-B on glucose uptake in skeletal muscle, focusing on glucose uptake by skeletal muscle cells and vascular endothelial cells.

Bilateral enhancement network with signal-to-noise ratio fusion for lightweight generalizable low-light image enhancement.

Low-light image enhancement aims to enhance the visibility and contrast of low-light images while eliminating complex degradation issues such as noise, artifacts, and color distortions. Most existing low-light image enhancement methods either focus on quality while neglecting computational efficiency or have limited learning and generalization capabilities. To address these issues, we propose a Bilateral Enhancement Network with signal-to-noise ratio fusion, called BiEnNet, for lightweight and generalizable low-light image enhancement.

Hydrophilic Metal-Organic Frameworks Regulated by Biomineralized Protein for Enhanced Stability and Drug Delivery.

For bridging the gap between biological and synthetic materials, the fusion of Metal-Organic Frameworks (MOFs) with biological entities has emerged as a revolutionary strategy in functional materials. In this context, our study introduces a novel structure wherein Bovine Serum Albumin (BSA), a robust and versatile protein, encapsulates zeolitic imidazolate framework-8 (ZIF-8), forming a protein-caged MOF. Highlighting the advantages of this innovative design, the protein-encapsulation enhances the stability and dispersity of ZIF-8, and aids in the synthesis of smaller-sized nanoparticles, crucial for size-impact performance applications.

New 24-Membered Macrolactines from an Arctic Bacterium SCSIO 41392 and Their Anti-Pathogenicity Evaluation.

Three new 24-membered macrolactines, amylomacrolactines A-C (-), along with two known compounds and , were isolated from the Arctic bacteria SCSIO 41392. The configurations of - were assigned by a combination of coupling constants, NOESY, and analysis of MM2-optimized conformation, as well as by comparison with reports in the literature. Compounds and showed quorum sensing (QS) inhibitory activities against the PQS system and suppressed PQS-regulated virulence factor pyocyanin synthesis.

Robust single modified divacancy color centers in 4H-SiC under resonant excitation.

Color centers in silicon carbide (SiC) offer exciting possibilities for quantum information processing. However, the challenge of ionization during optical manipulation leads to charge variations, hampering the efficacy of spin-photon interfaces. Recent research predicted that modified divacancy color centers can stabilize their charge states, resisting photoionization.