[Polymorphism of exon 10 of prolactin receptor gene and its relationship with prolificacy of Jining Grey goats].

Prolactin receptor (PRLR) gene was studied as a candidate gene for the prolificacy of Jining Grey goats. Five pairs of primers were designed to detect single nucleotide polymorphisms of exon 10 and part of 3'untranslated region (UTR) of PRLR gene in both high fecundity breed (Jining Grey goat) and low fecundity breeds (Liaoning Cashmere goat, Boer goat and Angora goat) by PCR-SSCP. The nucleotide sequence of exon 10 and part of 3'UTR of caprine PRLR gene was spliced in this study for the first time.

Expression of LPL in endothelial-intact artery results in lipid deposition and vascular cell adhesion molecule-1 upregulation in both LPL and ApoE-deficient mice.

Objective: Overexpression of lipoprotein lipase (LPL) in deendothelialized artery led to profound localized lipid deposition. In this study the role of LPL in atherogenesis in endothelial-intact carotid arteries was assessed in genetically hyperlipidemic LPL- and ApoE-deficient mice.

Methods And Results: Human wild-type LPL (hLPLwt), catalytically inactive LPL (hLPL194), or control alkaline phosphatase (hAP) were expressed in endothelial-intact carotid arteries via adenoviral vectors.

Localized vessel expression of lipoprotein lipase in rabbits leads to rapid lipid deposition in the balloon-injured arterial wall.

Recent studies on mice demonstrated that lipoprotein lipase (LPL) located in the arterial wall might play a pro-atherogenic role. There are major differences between humans and mice in lipoprotein metabolism and in susceptibility to atherosclerosis. We have therefore used rabbits fed normal chow diet as a model to assess such localized effects by adenovirus-mediated gene transfer of human catalytically active wild type LPL (hLPLwt) and an inactive mutant (hLPL194) to balloon-injured carotid arteries.

[Microflora on mucosa under different pontics of fixed partial bridge].

Objective: To investigate the influence of three pontic types on alveolar ridge mucosal microecosystem.

Methods: Sixty patients ready to accept three unit metal ceramic bridges were selected. The bacterial type and the cultivable flora were counted and the proportions of bacteria detected on the top of alveolar ridge mucosal contact area before tooth preparation and three months after bridge insertion.

[Study on BMP15 and GDF9 as candidate genes for prolificacy of Small Tail Han sheep].

Bone morphogenetic protein 15 (BMP15) gene and growth differentiation factor 9 (GDF9) gene which control the fecundity of Belclare and Cambridge ewes were studied as candidate genes on the prolificacy of Small Tail Han sheep. Single nucleotide polymorphism of GDF9 gene and BMP15 gene was detected in both high fecundity sheep breeds (Small Tail Han sheep and Hu sheep) and low fecundity sheep breeds (Dorset sheep, Texel sheep and German Mutton Merino sheep) by PCR-RFLP. The results showed that the G8 mutation (C --> T) of GDF9 gene and the B4 mutation (G --> T) of BMP15 gene were not detected in five sheep breeds.

[Research progress on myogenin gene].

In this paper we briefly introduced the location, structure, polymorphisms of myogenin gene, and discussed the relationships of myogenin gene with economic traits.

[PCR-SSCP analysis on growth differentiation factor 9 gene in sheep].

Growth differentiation factor 9 (GDF9) is a growth factor secreted by oocytes in growing ovarian follicles, which is essential for growth and differentiation of early ovarian follicles. The polymorphism of GDF9 gene in Small Tail Han sheep, Hu sheep, Dorset sheep and Suffolk sheep was analyzed by PCR-SSCP. The results indicated that there were three genotypes (AA, AB and BB) detected by primer 1.