Clinical study of ligustrazine combined with radial extracorporeal shock wave therapy in stroke patients with upper limb spasticity.

Upper limb spasticity is a common and serious sequelae in patients with hemiplegia after stroke. Radial extracorporeal shock wave therapy (rESWT) can improve upper limb dysfunction and relieve pain in patients, However, its efficacy as a single treatment is limited. In traditional Chinese medicine, Tetramethylpyrazine (TMP) is the main pharmacological component of Ligusticum chuanxiong.

CBX3 promotes multidrug resistance by suppressing ferroptosis in colorectal carcinoma via the CUL3/NRF2/GPX2 axis.

Chemoresistance poses a significant challenge in colorectal cancer (CRC) treatment. However, the mechanisms underlying chemoresistance remain unclear. CBX3 promoted proliferation and metastasis in CRC.

Research progress on estrogen and estrogen receptors in the occurrence and progression of autoimmune thyroid diseases.

Autoimmune thyroid disease (AITD) is a category of disease related to sex differences, with a significantly higher incidence in women than in men. In addition to X chromosome inactivation abnormalities, Estrogen and estrogen receptors may lead to the sex differences in AITD. Estrogen, estrogen receptors and estrogen receptor-mediated signaling pathways can affect the number and function of immune cells and the function of the thyroid to promote the development of AITD.

The Epidemiological Analysis of Respiratory Virus Infections in Children in Hangzhou from 2019 to 2023.

To investigate the infection of children with respiratory tract infection in Hangzhou from 2019 to 2023, and to explore the epidemiological characteristics of respiratory tract virus infection before and after the prevalence of novel coronavirus. A retrospective analysis was conducted on oral and pharyngeal swabs from 302,680 children with acute respiratory infections. Colloidal gold-based assays were used to detect viral antigens, including adenovirus (ADV), influenza A (FluA), influenza B (FluB), and respiratory syncytial virus (RSV).

Genome-wide analysis of KCS genes in tomato and functional characterization of SlKCS8 and SlKCS10 in drought tolerance.

KCS, an endogenous cellular enzyme, catalyzes the elongation of fatty acid chains and plays a crucial role in the biosynthesis of plant epidermal wax. Through processes such as transfer, decarboxylation, and fixation, it contributes to plant growth and adaptation to abiotic and biotic stresses. However, the mechanism by which KCS genes participate in the response of tomato plants to driught remains unclear.

Low-calorie diets and remission of type 2 diabetes in Chinese: phenotypic changes and individual variability.

Background: Chinese have distinct phenotypes of type 2 diabetes (T2D) and obesity compared with people of other ethnicities, but using low-calorie diets to achieve T2D remission has never been conducted in Chinese. This study aimed to assess if T2D remission can be achieved using low-calorie formula diet (LCFD) and low-calorie real food-based diet (LCRFD) in Chinese similarly to other populations and to identify determinants of individual variability in T2D remission.

Methods: This 6-month intervention consisted of a 3-month isocaloric intensive weight loss phase (815-835 kcal/d) and a 3-month weight maintenance phase.

Miniaturized Separation-Sensing Tandem Enabled by Fluorescent Monoliths.

Discriminative sensing for mixture by either fluorescent sensor or other sensors remains a challenge. This issue is attributed to the absence of a separation unit prior to the sensor. Here, we report a new sensing medium, named porous fluorescent monolith, which can separate mixture and meanwhile be integrated into a sensor for discriminative detection.

Bioinformatics analysis, immunogenicity, and therapeutic efficacy evaluation of a novel multi-stage, multi-epitope DNA vaccine for tuberculosis.

Background: The global tuberculosis (TB) epidemic remains severe. We aimed to develop a therapeutic DNA vaccine as an adjunct to TB treatment to improve efficacy.

Methods: The W545 DNA vaccine was constructed using the M.

Second harmonic and sum frequency generation in silicon nitride microring on thin-film lithium niobate.

We demonstrate significant second harmonic generation (SHG) and sum frequency generation (SFG) on a thin-film lithium niobate (TFLN) platform by loading a silicon nitride (SiN) microring resonator, which avoids the dry etching of lithium niobate. By optimizing SiN-loaded TFLN waveguide geometry, the phase-matching conditions between the fundamental pump laser and the SHG/SFG signals are designed. Combining with enhancement by the microring resonator, remarkable SHG and SFG signals with conversion efficiencies of 16.

Myocardial intramural course may reduce left ventricular ejection fraction of patients suffering from coronary heart disease.

Background: Myocardial intramural course (MIC), a benign anatomical lesion, is an abnormal anatomical structure formed due to abnormal blood vessel routing. An increasing number of studies indicate that MIC is associated with coronary heart disease (CHD). However, it remains unclear whether MIC contributes to cardiac function impairment in patients with CHD.

Applications of membrane technology in the resource recovery of power lithium-ion battery precursor wastewater: A review.

New energy materials and technologies are developing rapidly, and the sales of electric vehicles are increasing significantly. The cathode material is the core of electric vehicle batteries. The production of electrode precursors generates a large amount of wastewater.

SmCSN5 is a synergist in the transcription factor SmMYB36-mediated biosynthesis of tanshinones and phenolic acids in .

The ubiquitin-26S proteasome system (UPS) is associated with protein stability and activity, regulation of hormone signaling, and the production of secondary metabolites in plants. Though the mechanism of action of SmMYB36 on the tanshinone and phenolic acid biosynthesis is well understood, its regulation through post-translational modifications is unclear. A constitutive photomorphogenesis 9 (COP9) signalosome subunit 5 (SmCSN5), which interacted with SmMYB36 and inhibited its ubiquitination-based degradation, was identified in .

Cutting-Edge Therapy and Immune Escape Mechanisms in EBV-Associated Tumors.

Epstein-Barr virus (EBV), the first identified human tumor virus, significantly influences the immune microenvironment of associated cancers. EBV-induced expression of viral antigens by tumor cells triggers immune recognition and elicits a pro-inflammatory response. While mild inflammation may help eliminate malignant cells, intense inflammation can accelerate tumor progression.

Eye-sparing treatment for sinonasal tumors invading the orbit in the era of personalized therapy.

Few studies investigated the eye-sparing treatment in patients with sinonasal carcinomas (SNCs) from the perspective of modern precision radiation, and the eye-sparing treatment in patients with grade III orbital invasion was rarely discussed. This retrospective study included patients with primary SNCs who accepted eye-sparing treatment from October 2000 to June 2022 at a single institution. Patients' clinical information and follow-up data (including visual data) were obtained.

Canagliflozin Attenuates Podocyte Inflammatory Injury through Suppressing the TXNIP/NLRP3 Signaling Pathway in Diabetic Kidney Disease Mice.

Diabetic kidney disease (DKD), a leading cause of end-stage renal disease (ESRD), poses a serious threat to global health. Aseptic inflammation and pyroptosis of podocytes are crucial factors contributing to the pathogenesis and progression of DKD. Sodium-glucose cotransporter 2 inhibitors (SGLT2i), a novel class of antidiabetic agents widely used in clinical settings, may exert a protective effect on podocyte injury, although the underlying mechanisms remain poorly understood.

Revealing the Effects of Tympanic Membrane Implant Mechanical Properties on High-Frequency Hearing Loss After Clinical Myringoplasty: A Finite Element Analysis.

Myringoplasty is most commonly used to treat tympanic membrane (TM) perforation. Clinical data have shown that unexplained high-frequency (above 3 kHz) hearing loss often occurs after myringoplasty. In this paper, a finite element (FE) model of the partial external and middle ear (ME) of the human ear, which considers the actual perforation and TM implants, is developed to reveal the mechanical mechanism of high-frequency hearing loss after implantation of temporalis fascia and cartilage commonly used in myringoplasty.

The burden of migraine and tension-type headache in Asia from 1990 to 2021.

Background: In recent years, headache diseases have spread throughout the world, causing great suffering and even severe disability to patients, and increasing the burden on health care systems. However, studies of specific regions are rare. The purpose of our study is to comprehensively analyze the current situation and trends of headache diseases in Asia between 1990 and 2021, to provide details of headache diseases in Asia, and to provide scientific data to support health development strategies.

Prognostic relevance of immunoglobulin heavy chain rearrangement and immunoglobulin kappa light chain rearrangement in patients with diffuse large B cell lymphoma.

Purposes: Evidence has demonstrated that monitoring of the variable, diversity, and joining gene segments (VDJ) rearrangement of immunoglobulin (Ig) gene in the circulating tumor DNA (ctDNA) is highly valuable in predicting the prognosis of patients with diffuse large B cell lymphoma (DLBCL). In this study, we investigated the role of both Ig heavy chain (IGH) and Ig kappa light chain (IGK) gene rearrangements detected in ctDNA samples in predicting DLBCL progression.

Methods: Next-generation sequencing (NGS) was used to identify the dominant V(D)J clonotypic rearrangement in tissue samples of 33 DLBCL patients.

Artificial Intelligence Versus Rules-Based Approach for Segmenting NonPerfusion Area in a DRCR Retina Network Optical Coherence Tomography Angiography Dataset.

Purpose: Loss of retinal perfusion is associated with both onset and worsening of diabetic retinopathy (DR). Optical coherence tomography angiography is a noninvasive method for measuring the nonperfusion area (NPA) and has promise as a scalable screening tool. This study compares two optical coherence tomography angiography algorithms for quantifying NPA.

Efficacy and Safety of Unilateral Biportal Endoscopy Compared With Transforaminal Route Percutaneous Endoscopic Lumbar Decompression in the Treatment of Lumbar Spinal stenosis: Minimum 1-year Follow-up.

Objective: This study compared the efficacy and safety of unilateral biportal endoscopic (UBE) decompression and transforaminal route percutaneous endoscopic lumbar decompression (PELD) for lumbar spinal stenosis (LSS), assessing 1-year clinical outcomes.

Methods: A total of 120 patients (64 UBE, 56 PELD) diagnosed with LSS in 2021 were evaluated. Perioperative outcomes included overall operation time, extracanal operative time, intracanal decompression time, incision length, fluoroscopy time, estimated blood loss, preoperative and postoperative day 3 hb levels, length of post-operative hospital stays, total expenses, postoperative complications.

Crystal structure of the S-adenosylmethionine-dependent mycolic acid synthase UmaA from Mycobacterium tuberculosis.

Mycobacterium tuberculosis is a Gram-positive bacillus that causes tuberculosis and is a leading cause of mortality worldwide. This disease is a growing health threat due to the occurrence of multidrug resistance. Mycolic acids are essential for generating cell walls and their modification is important to the virulence and persistence of M.

Observation of switchable polar skyrmion bubbles down to the atomic layers in van der Waals ferroelectric CuInPS.

Polar skyrmions are topologically nontrivial polarization textures that demonstrate exotic physical phenomena and novel memory applications. Thus far, these textures have primarily been reported in oxide-ferroelectric-based epitaxial heterostructures because their stabilization requires an elastic energy penalty from the epitaxial strains. Here, without the epitaxial-strain engineering, we discover polar skyrmion bubbles in stand-alone van der Waals ferroelectric CuInPS crystal through the combination of piezoelectric force microscopy, high-resolution transmission electron microscopy, and phase-field simulations.

SKA3 promotes lung adenocarcinoma progression via the EGFR/E2F1/SKA3/integrin β1 signaling loop.

Spindle and kinetochore-associated complex subunit 3 (SKA3) contributes to tumor growth and metastasis, but its specific roles have not been clearly elucidated. In this study, we found that SKA3 contributed to lung adenocarcinoma (LUAD) progression by interacting with integrin β1. The expression characteristics of SKA3 in LUAD patients were analyzed by The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) datasets and validated in 33 paired LUAD tissues by immunohistochemistry.

POSA for fast, amplified and multiplexed protein imaging.

Fluorescent π-conjugated polymers (FCPs) are known for their superior brightness but are still unavailable for highly multiplexed molecular imaging in single cells as they are hydrophobic and lack targeting capability toward biomolecules. Herein, we develop a π-conjugated polymer-based amplification (POSA) method to achieve highly multiplexed signal amplification. Optical amplification by virtue of the high brightness of FCPs makes POSA a simple and quick signal amplification technique that can spatially resolve the distribution of multiplexed proteins in single cells, with a 28- to 126-fold signal amplification effect.

Prioritizing microbial functions over soil quality for enhanced multifunctionality in saline-sodic soil remediation.

Paddy cultivation has become a widely adopted approach for saline-sodic wasteland reclamation, aiming to mitigate the food crisis and enhance soil quality. Nevertheless, the impact of long-term paddy cultivation on the interplay between soil quality, microbial metabolic functions, and soil ecosystem multifunctionality (EMF) remains unclear. Here, we evaluated soil physicochemical properties, the abundance of 132 biomarker functional genes, and soil EMF across a 78-year period of saline-sodic paddy cultivation.