Daytime dysfunction may be associated with postoperative delirium in patients undergoing total hip/knee replacement: The PNDABLE study.

Purpose: Postoperative delirium (POD) is a usual complication after total hip/knee replacement, which may be affected by sleep characteristics. However, up to now, preoperative sleep characteristics have not been evaluated as risk factors of POD. The relationship between self-reported sleep characteristics and POD in patients has been investigated in this study.

[Efficacy of dermal scaffold for promoting repair of acute full-thickness skin defects in pigs].

Objective: To investigate the efficacy of Lando dermal scaffold for promoting repair of acute full-thickness skin defects in pigs and explore the possible mechanism.

Methods: Three 5 cm×5 cm full-thickness skin defects were created on the left dorsal skin (control group) and another 3 on the right dorsal skin (treatment group) of each of 6 Tibetan pigs. The wounds in the treatment group were covered with a bilayer artificial skin (Lando) and the control wounds with vaseline gauze.

[Effects of vacuum drainage combined with heparin irrigation for treatment of scald burns with seawater immersion in rabbits].

Objective: To investigate the effect of closed vacuum drainage combined with heparin irrigation in the treatment of scald burns with seawater immersion in rabbits.

Methods: Twenty New Zealand rabbits were subjected to deep partial-thickness scald burns in 4 regions on the bilateral skin of the spine. The wounds were managed with common dressing (group A), common dressing after seawater immersion (group B), closed vacuum drainage after seawater immersion (group C), or closed vacuum drainage combined with heparin irrigation after seawater immersion (group D).

Expression of vascular endothelial growth factor in deep second-degree scald wounds in rats.

Objective: To observe the expression of vascular endothelial growth factor(VEGF)in deep second-degree scald wounds,with an attempt to further explore the role of VEGF in burn wound healing.

Methods: Totally 36 adult Wistar rats were randomized into two groups: the scald group(30 rats)and the control group(6 rats). In the scald group,rat models of deep second-degree scald wounds were established.

[Expression of aquaporin-1 in myocardium of rats in the early stage after scald and its relationship with myocardial edema].

Objective: To investigate the changes in aquaporin-1 (AQP-1) expression in myocardium of scalded rats in early stage of a burn injury, and to analyze its relationship with myocardial edema.

Methods: Thirty-six healthy Wistar rats were divided into normal control (n = 6, without scald) and scald (n = 30) groups according to the random number table. Rats in scald group were inflicted with 30%TBSA full-thickness scald on the back, and intraperitoneally injected with Ringer's solution for antishock treatment.

[Clinical observation on repairing of wounds of skin graft donor site with acellular tissue engineering dermal matrix].

Objective: To evaluate the clinical efficacy of acellular tissue engineering dermal matrix (ATDM) in repairing wounds of skin graft donor site.

Methods: Sixty patients with burn or chronic wounds hospitalized from January 2011 to April 2012 received autologous skin grafting. One wound [with size larger than 55 cm(2), and thickness of (0.

[Treatment of a patient with massive unhealed wound accompanied by wound sepsis in late stage after burn injury].

This article analyzed the medical records of a patient with 90% TBSA unhealed wound accompanied with wound sepsis 50 days post burn (PBD) and to discuss the ideal strategies of treatment for such patients in such condition. This was a 24-year-old male patient suffering from flame burn with 95% TBSA wound and severe inhalation injury. Meek skin grafting with autologous scalp was performed once to the thoracic and abdominal regions; intermingled skin grafting of autologous scalp microskin and large sheet of allograft was performed twice to the limbs within PBD 31.

[Effects of exendin-4 on rat cardiomyocyte apoptosis early after severe scald injury].

Objective: To observe the effect of exendin-4 on cardiomyocyte apoptosis in the early stage after scald injury in rats and explore the mechanisms.

Methods: Fifty-four healthy adult SD rats were randomly divided into normal control group (n=6), scald group (n=24) and scald with exendin-4 treatment group (n=24). In the latter two groups, the rats were subjected to 30% TBSA full-thickness scald burns on the back, and Parkland formula was used for determining the resuscitation fluid volume.

[Study on cotransfection of genes of insulin-like growth factor I and herpes simplex virus thymidine kinase for optimization of wound healing].

Objective: To study the effect of cotransfection of genes of insulin-like growth factor I (IGF-I) and herpes simplex virus thymidine kinase (HSV-tk) on wound healing.

Methods: Thirty male Wistar rats were inflicted with 30% TBSA full-thickness scald. They were then divided into A group (4.

[Changes of aquaporin-1 expression in rat myocardium after severe burn].

Objective: To investigate the changes in the myocardial expression of aquaporin-1 (AQP1) protein and its association with myocardial edema in rats with severe burns.

Methods: Forty-eight healthy adult Wistar rats were randomly divided into normal control group (n=6) and burn injury group with third degree burn of 30% total body surface area, and the latter group was further divided into 2, 4, 8, 12, 24, 48 and 72 h groups. The changes of myocardial water content were investigated by dry-wet weight methods.

[Effect of early enteral feeding on the expression of aquaporin 1 in edematous small intestinal tissues of rats after severe burn].

Objective: To investigate the changes in the expression of aquaporin 1 (AQP-1) in edematous small intestinal tissues of rats after severe burn and the effect of early enteral feeding on its expression.

Methods: Ninety normal adult Wistar rats were randomly divided into normal control group (n=6), burn model group (n=42, with 30% TBSA III degrees) and early feeding group (n=42). Dry weight method, ELSIA and immunohistochemistry were used to observe and detect the water content and expression of AQP-1 in the intestinal tissue at 1, 4, 8, 12, 24, 48, and 72 h after the burns.

Double-edged effects of neuropeptide substance P on repair of cutaneous trauma.

To explore further the role of substance P (SP) in wound healing and scar formation, SP concentrations in wounds of scalded rats were assayed. Expressions of apoptosis-associated genes in fibroblasts cultured with SP were detected. SP concentrations in superficial wounds increased earlier than those in deep wounds.

[Effect of liposome-mediated HSV-tk gene transfer on fibroblast apoptosis in rats with scald injury].

Objective: To evaluate the effect of HSV-tk gene transfer on the apoptosis of fibroblast in rats with scald injury.

Methods: The recombinant eukaryotic expression vector pcDNA3.1/tk was transfected via liposome in the skin of rats with scald injury.

[Effect of local treatment with cooling and spray film on early edema of superficial II degree scald burns in rats].

Objective: To investigate the anti-inflammatory effect of early local treatment with cooling and spray film on scald burn injury in rats.

Method: Seventy-five Wistar rats were randomly divided into 5 groups including the sham-scalded group, untreated scald group, cooling group, spray film group, and cooling plus spray film group with corresponding treatments. After gross observation of the wounds, the tissues at the wounds were sampled at different time points after the injury to determine the total water content (wet: dry weight ratio) and prostaglandin E(2) (PGE(2)) levels using radioimmunoassay (RIA).

[Influence of epidermis from different sources on the proliferation and collagen content of fibroblasts].

Objective: To investigate the influence of epidermis from different sources on the proliferation and metabolism of fibroblasts (Fb), and to explore its cause.

Methods: In a co-culture system, normal Fb (A group) and cicatricial Fb(B group) from 10 patients with scar during proliferative stage were co-cultivated with own normal skin epidermis (NSE), respectively, without direct contact. In control groups (C group), cicatricial Fb was cultured alone.

[Effect of nuclear factor-kappaB activation on expression of proinflammatory cytokines in rat lung tissues in early stage of burn injury].

Objective: To investigate the effect of nuclear factor-kappaB (NF-kappaB) activation on the expression of proinflammatory cytokines in the lung tissues of rats with early-stage burn injury.

Methods: Wistar rats were randomly divided into 3 groups, namely the normal control, burn, burn and PDTC treatment groups, and in the latter two groups, the rats were subjected to 35% TBSA full-thickness burns. Activation of pulmonary NF-kappaB at 1, 3, 6, 12, and 24 postburn hour (PBH) was tested by electrophoretic mobility shift assay , and the expressions of pulmonary tumor necrosis factor alpha (TNF alpha) and interleukin-8 (IL-8) mRNAs at 3, 6, 12, and 24 h were detected by RT-PCR.

[Sequential management of residual wounds in burn patients].

Objective: To seek a sequential method for the management of residual wounds in burn patients.

Methods: Three chronic residual wounds on each of 25 burn patients were either covered with vaseline gauze (A group), human tissue-engineered active skin (Active Skin, B group) or Active Skin after rinsing with fluid containing oxygen and vacuum assisted drainage ( C group) on wounds. The contents of (TNF)a in granulation tissue were assayed by enzyme linked immunosorbent assay (ELISA).

[Effect of NF-kappaB activation on the early expression of tumor necrosis factor alpha and myocardial dysfunction in burned rats].

Objective: To investigate the effect of NF-kappaB activation on the early expression of proinflammatory cytokines in myocardium and early myocardial dysfunction in burn rats.

Methods: One hundred and seventy Wistar rats were enrolled in the study and randomly divided into three groups, i. e.

[Experimental study of gene transfer of insulin-like growth factor I on wound healing of scald rats].

Objective: To probe into the effect of IGF-I gene transfer on wound healing of scald rats.

Methods: Eukaryotic recombinant plasmid of pcDNA3.1/ IGF-I (successfully constructed by our study group earlier) transported by liposome were injected subdermally in wound region of scald rats.

[The role of nuclear factor-kappaB in the burn serum-induced expression of intercellular adhesion molecules-1 in endothelial cells].

Objective: To explore the role of nuclear factor-kappaB (NF-kappaB) in the burn serum induced expression of intercellular adhesion molecules-1 (ICAM-1) in endothelial cells.

Methods: Human umbilical vein endothelial cells (HUVECs) were cultured in vitro and divided into control (with normal serum stimulation), burn serum (B, with burn serum stimulation) and PDTC (with burn serum and PDTC stimulation) groups. The NF-kappaB activity in endothelial cells was determined with electrophoretic mobility shift assay (EMSA) at 0.

[Influence of substance P on the proliferation and apoptosis of fibroblasts of pathological scars].

Objective: To further explore the effects of substance P on the proliferation and apoptosis of fibroblasts obtained from pathological scars in vitro.

Methods: Fibroblasts from keloid (KSF) , hypertrophic scar (HSF) and normal dermis (NDF) of 12 burn patients were cultured in vitro and divided into control, SP (with 1 x 10 (-6) mol/L SP added to the culture medium) , and SP + spantide( with 1 x 10 (-6) mol/L SP and 3 x 10 (-5) mol/L spantide added to the culture medium) groups. MTT method or flow cytometry assay was used for the determination of the proliferative activities or apoptotic rate of fibroblasts obtained from KSF, HSF and NDF with SP or Spantide.

[Influence of sea water immersion on inflammation and healing of the wounds in scalded rats].

Objective: To investigate the influence of sea water immersion on inflammation and healing of the wounds in scalded rats.

Methods: One hundred and forty-four male Wistar rats with 10% TBSA superficial partial-thickness scald were randomly divided into A (n=72, with scald) and B (n=72, with seawater immersion for 4hrs immediately after scald) groups. The serum contents of K+, Na+, Cl- were determined at 0 post-scald hour (PSH), 6PSH, 12PSH and 24 PSH with electrocyte analysis apparatus, and the changes in serum interleukin-6 (IL-6) and tumor necrosis factor (TNF-alpha) levels were determined at 0 PSH, 6PSH and 12 PSH with enzyme-linked immunosorbent assay (ELISA).

[Influence of nuclear factor-kappaB activation on the expression of cytokines in monocytes stimulated by burn serum].

Objective: To investigate the effects of NF-kappaB activation on the expression of cytokines in monocytes stimulated by burn serum, so as to explore the mechanism in monocyte activation by burn serum.

Methods: Peripheral blood monocytes (PBMCs) isolated from healthy volunteers were employed as the target cells. The cells were stimulated by serum from healthy volunteers (control), by serum from burn patients (burn serum), and by burn serum with addition of PDTC (pyrrolidine dithiocarbamate).

[Experimental study on the relationship between neuropeptide substance P and wound healing in scalded rats].

Objective: To explore the relationship between neuropeptide substance P (SP) and wound healing in scalded rats.

Methods: (1) Scalded rats with different degrees of scald injury were employed as the experimental model and were sacrificed at 24 post scald hour (PSH), and on 3, 7 and 14 post scald days (PSD). The SP content in the wound was detected with radioimmunoassay method.