Identification and characterization of a novel QTL for barley yellow mosaic disease resistance from bulbous barley.

Winter barley (Hordeum vulgare) production areas in the middle and lower reaches of the Yangtze River are severely threatened by barley yellow mosaic disease, which is caused by Barley yellow mosaic virus and Barley mild mosaic virus. Improving barley disease resistance in breeding programs requires knowledge of genetic loci in germplasm resources. In this study, bulked segregant analysis (BSA) identified a novel major quantitative trait loci (QTL) QRym.

Protein tyrosine phosphatase PTPH1 potentiates receptor tyrosine kinase HER2 oncogenesis via a PDZ-coupled and phosphorylation-driven scaffold.

Cancer cell overexpresses numerus proteins, however, how these up-regulated proteins, especially those enzymatically opposite kinases and phosphatases, act together to promote oncogenesis is unknown. Here, we reported that protein tyrosine phosphatase H1 (PTPH1) is a scaffold protein for receptor tyrosine kinase (HER2) to potentiate breast tumorigenesis. PTPH1 utilizes its PDZ domain to bind HER2, p38γ, PBK, and YAP1 and to increase HER2 nuclear translocation, stemness, and oncogenesis.

Donor Strand Complementation and Calcium Ion Coordination Drive the Chaperone-free Polymerization of Archaeal Cannulae.

Cannulae are tubular protein filaments that accumulate on the extracellular surface of the hyperthermophilic archaeon during cell division. Cannulae have been postulated to act as a primitive extracellular matrix through which cells could communicate or exchange material, although their native biological function remains obscure. Here, we report cryoEM structural analyses of cannulae and of protein assemblies derived from recombinant cannula-like proteins.

A Highly Efficient System for Separating Glandular and Non-glandular Trichome of Cucumber Fruit for Transcriptomic and Metabolomic Analysis.

Cucumber () trichomes play a critical role in resisting external biological and abiotic stresses. Glandular trichomes are particularly significant as they serve as sites for the synthesis and secretion of secondary metabolites, while non-glandular trichomes are pivotal for determining the appearance quality of cucumbers. However, current methods for separating trichomes encounter challenges such as low efficiency and insufficient accuracy, limiting their applicability in multi-omics sequencing studies.

Revisiting the role of light signaling in plant responses to salt stress.

As one of the grave environmental hazards, soil salinization seriously limits crop productivity, growth, and development. When plants are exposed to salt stress, they suffer a sequence of damage mainly caused by osmotic stress, ion toxicity, and subsequently oxidative stress. As sessile organisms, plants have developed many physiological and biochemical strategies to mitigate the impact of salt stress.

Oligo-FISH barcode chromosome identification system provides novel insights into the natural chromosome aberrations propensity in the autotetraploid cultivated alfalfa.

Alfalfa is one of the most economically valuable forage crops in the world. However, molecular cytogenetic studies in alfalfa lag far behind other cash crops and have reached a bottleneck. Here, we developed a novel chromosome identification system by designing 21 oligo probes in specific regions of each chromosome, which can be used as a barcode to simultaneously distinguish all chromosomes in a cell.

A standards-based application for improving platelet transfusion workflow.

Objective: Thrombocytopenia is a common complication of hematopoietic stem-cell transplantation (HSCT), though many patients will become immune refractory to platelet transfusions over time. We built and evaluated an electronic health record (EHR)-integrated, standards-based application that enables blood-bank clinicians to match platelet inventory with patients using data previously not available at the point-of-care, like human leukocyte antigen (HLA) data for donors and recipients.

Materials And Methods: The web-based application launches as an EHR-embedded application or as a standalone application.

A Nitrate/Nitrite Biosensor Designed with an Antiterminator for Diagnosis of Colitis Based on .

is a common microorganism in the human gut that has been linked to health benefits. Furthermore, it is an emerging synthetic biology chassis with the potential to be modified into diagnostic or therapeutic engineered probiotics. However, the absence of biological components limits its further applications.

Buffered Hydroxyl Radical for Photocatalytic Non-Oxidative Methane Coupling.

Hydroxy radical (•OH) is a prestigious oxidant that allows the cleavage of strong chemical bonds of methane but is untamed, leading to over-oxidation of methane and waste of oxidants, especially at high methane conversion. Here, we managed to buffer •OH in an aqueous solution of photo-irradiated Fe3+, where •OH almost participates in methane oxidation. Due to the interaction between Fe3+ and SO42-, the electron transfer from OH- to excited-state Fe3+ for •OH generation is retarded, while excessive •OH is consumed by generated Fe2+ to restore Fe3+.

Bifunctional probe propelling multipath strand displacement amplification tandem CRISPR/Cas12a for ultrasensitive and robust assay of DNA methyltransferase activity.

Background: DNA methylation catalyzed by various DNA methyltransferases (DNA MTases) is one of the important epigenetic regulations in both eukaryotes and prokaryotes. Therefore, the detection of DNA MTase activity is a vital target and direction in the study of methylation-related diseases.

Results: In this study, an ultrasensitive and robust strategy was developed for DNA MTase activity sensing based on bifunctional probe propelling multipath strand displacement amplification and CRISPR/Cas12a techniques.

Developing and experimental validating a T cell senescence-related gene signature to predict prognosis and immunotherapeutic sensitivity in non-small cell lung cancer.

Background: T cell senescence affects non-small cell lung cancer (NSCLC) by compromising the anti-tumor immune response. However, the prognostic significance of T cell senescence-related genes in NSCLC remains unclear.

Methods: The scRNA-seq data from normal lung and NSCLC tissues, along with co-incubation experiments involving NSCLC cells and T cells, were utilized to identify T cell senescence characteristics.

The impact of community-based, non-pharmaceutical interventions on anxiety and depression in fibromyalgia: A systematic review and network meta-analysis.

Background: Fibromyalgia syndrome (FMS) is often accompanied by anxiety and depression, seriously affecting the prognosis of patients. Active non-pharmacological therapies are the mainstay of treatment, but the optimal choice is still contentious.

Methods: We did a network meta-analysis(NMA) of RCTs and compared 7 community-based non-pharmacological interventions based on 29 studies.

Synthesis of polyethylene glycol bis-anti-cancer drug couplings and their anti-tumor activity based on tumor immune dynamic modeling.

In order to make the drugs can cure the tumor precisely, this paper establishes the tumor immune dynamic model through the differential equation of tumor growth and analyzes the persistence of the tumor immune model. Research on dual anticancer drugs and commonly used coupling methods is carried out to complete the synthesis of polyethylene glycol dual anticancer drug couplers and the antitumor activity is analyzed to derive the degree of inhibition of polyethylene glycol dual anticancer drugs on tumor activity. From the four judging criteria, it was concluded that the polyethylene glycol bis-anti-cancer drug has a better curative effect on tumor cells.

[Advances in the study of viruses inhibiting the production of advanced autophagy or interferon through Rubicon to achieve innate immune escape].

The innate immune response is the first line of defense for the host against viral infections. Targeted degradation of pathogenic microorganisms through autophagy, in conjunction with pattern recognition receptors synergistically inducing the production of interferon (IFN), constitutes an important pathway for the body to resist viral infections. Rubicon, a Run domain Beclin 1-interacting and cysteine-rich domain protein, has an inhibitory effect on autophagy and IFN production.

Advancement in synthetic gene circuits engineering: An alternative strategy for microRNA imaging and disease theranostics.

Gene circuits, which are genetically engineered systems designed to regulate gene expression, are emerging as powerful tools in disease theranostics, especially in mammalian cells. This review explores the latest advances in the design and application of gene circuits for detecting and treating various diseases. Synthetic gene circuits, inspired by electronic systems, offer precise control over therapeutic gene activity, allowing for real-time, user-defined responses to pathological signals.

Effect of non-pharmacological interventions on chemotherapy induced delayed nausea and vomiting for tumors: a systematic review and Bayesian network Meta-analysis.

Objective: To evaluate the efficacy of non-pharmacological interventions in improving chemotherapy induced delayed nausea and vomiting symptoms using a network meta-analysis.

Methods: Four Chinese databases (CNKI, Wanfang Data Knowledge Service Platform, VIP, Sinomed) and five English databases (PubMed, Cochrane Library, Embase, Web of Science, CINAHL) were searched from the establishment of the database to April 2024. A Bayesian network meta-analysis was performed on the response rate to the improvement of chemotherapy induced delayed nausea and vomiting, as well as improvement in KPS score, under different non-pharmacological interventions by using R 4.

Kan-AAE-driven synthetic SERS spectra generation method for Precise cancer identification.

Surface-Enhanced Raman Spectroscopy (SERS) is gaining popularity in cancer detection studies because it offers a non-invasive and rapid approach. Label-free SERS detection techniques often needs machine learning, which depends on adequate data for training. The scarcity of blood serum samples from cancer patients, due to challenges in collection linked to confidentiality concerns and other restrictions, can result in model overfitting and poor generalization ability.

Nitrous oxide analgesia for rehabilitation after anterior cruciate ligament reconstruction: A randomized controlled trial.

Background: Although there is increasing emphasis on rehabilitation training after ligament reconstruction, little is known about the pain induced by the procedure itself. Procedural success may be limited by pain and anxiety. Nitrous oxide is widely used to alleviate procedural pain.

The Prognostic Value of Sarcopenia in Clinical Outcomes in Cervical Cancer: A Systematic Review and Meta-Analysis.

Background: Sarcopenia is a condition characterized by inadequate muscle and function decline and is often associated with ageing and cancer. It is established that sarcopenia and muscle loss occurred during treatment are associated with the clinical outcomes of patients with cancer. This systematic review and meta-analysis aims to evaluate the association between sarcopenia at pretreatment and during treatment and overall survival or disease progression in patients with cervical cancer.

Transferrin-Based Bismuth Nanoparticles for Radiotherapy with Immunomodulation Against Orthotopic Glioma.

Modern radiotherapy frequently employs radiosensitizers for radiation dose deposition and triggers an immunomodulatory effect to enhance tumor destruction. However, developing glioma-targeted sensitizers remains challenging due to the blood-brain barrier (BBB) and multicomponent instability. This study aims to green-synthesize transferrin-bismuth nanoparticles (TBNPs) as biosafe radiosensitizers to enhance X-ray absorption by tumors and stimulate the immune response for glioma therapy.

Intelligent Optimization Design Framework for Alternating Current Pulse Modulation Electrohydrodynamic Printing Process Parameters.

To achieve efficient size tuning of printed microstructures on insulating substrates, an integrated process parameter intelligent optimization design framework for alternating current pulse modulation electrohydrodynamic (AC-EHD) printing is proposed for the first time. The framework is comprised of two stages: the construction of a prediction model and the acquisition of process parameters. The first stage employs the elk herd optimizer(EHO)-artificial neural network(ANN) to establish a mapping relationship between printing process parameters and the size of deposited droplets.

A Survey of Deep Anomaly Detection in Multivariate Time Series: Taxonomy, Applications, and Directions.

Multivariate time series anomaly detection (MTSAD) can effectively identify and analyze anomalous behavior in complex systems, which is particularly important in fields such as financial monitoring, industrial equipment fault detection, and cybersecurity. MTSAD requires simultaneously analyze temporal dependencies and inter-variable relationships have prompted researchers to develop specialized deep learning models to detect anomalous patterns. In this paper, we conducted a structured and comprehensive overview of the latest techniques in deep learning for multivariate time series anomaly detection methods.

Variable-Parameter Impedance Control of Manipulator Based on RBFNN and Gradient Descent.

During the interaction process of a manipulator executing a grasping task, to ensure no damage to the object, accurate force and position control of the manipulator's end-effector must be concurrently implemented. To address the computationally intensive nature of current hybrid force/position control methods, a variable-parameter impedance control method for manipulators, utilizing a gradient descent method and Radial Basis Function Neural Network (RBFNN), is proposed. This method employs a position-based impedance control structure that integrates iterative learning control principles with a gradient descent method to dynamically adjust impedance parameters.

Study on Fire Temperature Field in Small-Section Steel-Shell Concrete Immersed Tube Tunnel Structure Based on CFD-FEM Method.

Small-section steel-shell concrete immersed tube tunnels are intended for minibuses and have a low fire heat release rate. Standard fire rise curves do not apply to such tunnels. In this study, a coupled method of computational fluid dynamics (CFD) and the finite element method (FEM) was used to simulate the structural temperature distribution in tunnels.

Overexpression of from Bunge Enhanced Drought and Salt Tolerance by Improving ROS-Scavenging Capability.

() genes play a crucial role in the response to abiotic stress and are important target genes for research on plant stress tolerance mechanisms. Bunge is a promising candidate tree species for investigating the tolerance mechanism of woody plants against abiotic stress. In our previous study, was identified as being associated with seed drought tolerance.