Quantitative profiling of PTM stoichiometry by DNA mass tags.

Protein post-translational modification (PTM) serves as an important mechanism for regulating protein function. Accurate assay of PTM stoichiometry, or PTM occupancy, which refers to the proportion of proteins that contain specific modifications, is important for understanding the function of PTMs. We previously developed a novel chemoproteomic strategy "STO-MS" to quantify the PTM stoichiometry in complex biological samples, which employs a resolvable polymer mass tag to differentiate modified proteins and utilizes liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) techniques to measure PTM stoichiometry.

Modeling and Simulation of Micron Particle Agglomeration in a Turbulent Flow: Impact of Cylindrical Disturbance and Particle Properties.

The fly ash generated by coal combustion is one of the main sources of PM2.5, so the particulate matter removal technology of coal-fired boilers is receiving increasing attention. Turbulent agglomeration has emerged as a powerful tool for improving the efficiency of removing fine particulates from environments, sparking interest in its study.

Interfacial crack self-healing by Sporosarcina pasteurii: From medium optimization to spore encapsulation.

Self-healing cement takes advantage of microbial induced carbonate precipitation (MICP), a meritorious biological process, to achieve automatic healing of cement cracks. In this study, two beneficial factors, optimization of the bacteria culture medium and encapsulation of bacterial spores, were used to improve the MICP efficiency of Sporosarcina pasteurii in self-healing cement. On the one hand, in medium optimization, we compared the growth of Sporosarcina pasteurii fed with two generally used nitrogen sources, e.

The host restriction factor SERINC5 inhibits HIV-1 transcription by negatively regulating NF-κB signaling.

Serine incorporator 5 (SER5) can be incorporated into HIV-1 virions to block viral entry by disrupting the envelope glycoprotein-mediated viral fusion to the plasma membrane. Recent studies suggest that SER5 also inhibits HIV-1 mRNA transcription and the subsequent progeny virion biogenesis. However, the underlying mechanisms through which SER5 antagonizes the viral transcription remain poorly understood.

First Report of Leaf Spot Caused by on (Maxim) Komar. in China.

Artemisia stolonifera (Maxim.) Komar., also known as the 'elite of Artemisia', is currently widely planted in Jiangxi, Hubei, and other provinces in China.

A Review on Leaching of Spent Lithium Battery Cathode Materials Adopting Deep Eutectic Solvents.

As a result of the swift surge in the adoption of electric vehicles, the quantity of spent lithium-ion power batteries has been growing at an exponential rate. Improper handling of these batteries can lead to the waste of strategic metal resources and pose risks to the environment and human health. Without doubt, it is essential to scientifically recover and reuse these spent power batteries, particularly by recovering positive electrode materials.

growth of MnO nanoparticles on supramolecular polyaniline as chiral nanozymes for effective enantioselective catalysis.

growth of MnO nanoparticles (NPs) onto chiral /-polyaniline (/-PANI) scaffolds efficiently regulates the supramolecular chirality, making them function as chiral nanozymes for controllable enantioselective catalysis. Compared with the loaded MnO NPs onto /-PANI scaffolds, the enantioselectivity of the growth system is significantly enhanced due to the stronger bonding between catalytic centers and supramolecular scaffolds.

Comparative Metabolome and Transcriptome Analyses Reveal Molecular Mechanisms Involved in the Responses of Two Varieties to Salt Stress.

Salt stress severely inhibits crop growth and production. The native turfgrass species in northern China, exhibits extraordinary tolerance to multiple abiotic stresses. However, little is known about its specific metabolites and pathways under salt stress.

Fracture Mapping in High-Energy Chest Trauma.

: High-energy chest trauma often results in rib fractures and associated chest injuries. This study explored fracture distribution patterns in high-energy chest trauma, using three-dimensional (3D) fracture mapping technology. : This retrospective study analyzed cases of high-energy chest trauma with rib fractures treated at a Level 1 Trauma Center, from February 2012 to January 2023.

A simplified and efficient extracellular vesicle-based proteomics strategy for early diagnosis of colorectal cancer.

Colorectal cancer (CRC) is a major cause of cancer-related death worldwide and an effective screening strategy for diagnosis of early-stage CRC is highly desired. Although extracellular vesicles (EVs) are expected to become some of the most promising tools for liquid biopsy of early disease diagnosis, the existing EV-based proteomics methods for practical application in clinical samples are limited by technical challenges in high-throughput isolation and detection of EVs. In the current study, we have developed a simplified and efficient EV-based proteomics strategy for early diagnosis of CRC.

Fast and accurate DNASeq variant calling workflow composed of LUSH toolkit.

Background: Whole genome sequencing (WGS) is becoming increasingly prevalent for molecular diagnosis, staging and prognosis because of its declining costs and the ability to detect nearly all genes associated with a patient's disease. The currently widely accepted variant calling pipeline, GATK, is limited in terms of its computational speed and efficiency, which cannot meet the growing analysis needs.

Results: Here, we propose a fast and accurate DNASeq variant calling workflow that is purely composed of tools from LUSH toolkit.

Chemical Recording of Pump-Specific Drug Efflux in Living Cells.

Drug efflux-a process primarily facilitated by efflux pumps such as multidrug resistance proteins (MRPs)-plays a pivotal role in cellular resistance to chemotherapies. Conventional approaches to assess drug efflux are predominantly conducted in vitro and often lack pump specificity. Here we report the bioorthogonal reporter inhibiting efflux (BRIEF) strategy, which enables the recording of pump-specific drug efflux in living cells.

NIR-Activable Charge Transfer Agents for Synergistic Photoimmunotherapy.

The combination of photothermal therapy (PTT) and photodynamic therapy (PDT) has become an attractive tumor treatment modality, yet the facile design of photoimmunotheranostic agents with efficient near infrared (NIR) light-absorbing and immune-activating capabilities remains a tremendous challenge. Herein, we developed a NIR-activable organic charge transfer complex (CTC), with perylene (PER) as the electron donor and 4,5,9,10-tetrabromoisochromeno [6,5,4-def]isochromene-1,3,6,8-tetraone (Br4NDI) as the electron acceptor. Through further supramolecular assembly, the PER-Br4NDI nanoparticle (PBND NP) for spatiotemporally controlled photoimmunotherapy was constructed.

Molecular Structures of Poly(methyl methacrylate) at Different Buried Interfaces Revealed by Sum Frequency Generation Vibrational Spectroscopy.

Silica or calcium fluoride (CaF) substrate-supported poly(methyl methacrylate) (PMMA) thin films as insulating layers are commonly used in photoelectric/photovoltaic devices to improve the efficiency or stability of these devices. However, a comparative investigation of molecular structures at buried PMMA/silica and PMMA/CaF interfaces under thermal stimuli remains unexplored. In this study, we qualitatively and quantitatively revealed different molecular orderings and orientations of PMMA at two interfaces before and after annealing using sum frequency generation (SFG) vibrational spectroscopy.

Environmental, social and governance (ESG) performance as a moderator in assessing the impact of government subsidies on underinvestment in China.

In recent years, underinvestment has become common among subsidised enterprises in China. Stakeholder theory asserts that environmental, social and governance (ESG) performance forces enterprises to prioritise stakeholder needs by incorporating ESG principles into business operations, which in turn influences business investment decision-making. This study examines the impact of government subsidies on firm underinvestment and whether ESG performance plays a moderating role in this direct relationship.

In situ chemoproteomic profiling reveals itaconate inhibits de novo purine biosynthesis in pathogens.

Itaconate serves as an immune-specific metabolite that regulates gene transcription and metabolism in both host and pathogens. S-itaconation is a post-translational modification that regulates immune response; however, its antimicrobial mechanism under the physiological condition remains unclear. Here, we apply a bioorthogonal itaconate probe to perform global profiling of S-itaconation in living pathogens, including S.

Strain-Release-Driven Electrochemical Skeletal Rearrangement of Non-Biased Alkyl Cyclopropanes/Butanes.

Capitalizing the inherent strain energy within molecules, strain-release-driven reactions have been widely employed in organic synthesis. Small cycloalkanes like cyclopropanes and cyclobutanes, with their moderate ring strain, typically require dense functionalization to induce bias or distal activation of (hetero) aromatic rings via single-electron oxidation for relieving the tension. In this study, we present a pioneering direct activation of alkyl cyclopropanes/butanes through electrochemical oxidation.

Integrated transcriptomic and metabolomic analysis reveals the molecular profiles of dynamic variation in var. suffering from bulb rot.

var. , known as Longya lily, is a well-known medicinal and edible plant in China. Bulb rot is a common disease in Longya lily cultivation that severely affects the yield and quality of lilies.

Epiphytic Patterns Impacting Metabolite Diversity of Rhizomes Based on Widely Targeted Metabolomics.

Nakaike, a fern widely distributed in China and some countries in Southeast Asia, is a commonly used herbal medicine in tonic diets and Chinese patented medicine. The metabolites of its dried rhizomes are easily affected by the epiphytic pattern, whether on rock tunnels (RTs) or tree trunks (TTs). The current research focused on rhizomes from these two patterns, RTs and TTs (further divided into subclasses TA, TB, TC, and TD, based on trunk differences) and conducted a widely targeted metabolomics analysis.

Flt3 ligand augments immune responses to soluble PD1-based DNA vaccine via expansion of type 1 conventional DCs.

DNA vaccines are prospective for their efficient manufacturing process, but their immunogenicity is limited as they cannot efficiently induce CD8 T cell responses. A promising approach is to induce cross-presentation by targeting antigens to DCs. Flt3L can expand the number of type 1 conventional DCs and thereby improve cross-presentation.