Sequential cultivation method for β-fructofuranosidase production from URM4634, evaluation of their biochemical and kinetic/thermodynamic characteristics, and application on sucrose hydrolysis.

The present study focused on evaluating the sequential fermentation (SF) method for FFase production from URM4634 using soybean bran as substrate. The SF was performed using soybean bran as substrate at 72 h and 30 °C and the maximum hydrolytic activity (44.00 U mL), corresponding to an increase of 2.

Optimization strategy for inulinase production by URM5741 and its biochemical characterization, kinetic/thermodynamic study, and application on inulin and sucrose hydrolysis.

Inulinases are enzymes of great interest in the food industry, especially due to their application in the synthesis of fructose and fructo-oligosaccharides. Moreover, some inulinases () also present invertase activity (), making them useful for sucrose hydrolysis processes. In the present study, the production of inulinase by URM5741 was evaluated and optimized using two statistical approaches.