Diagnostic assessment of Mycoplasma genitalium in culture-positive women.

Detection of Mycoplasma genitalium-mediated, chlamydia-negative nongonococcal urethritis and other M. genitalium-linked infectious etiologies has been very challenging. Although M.

Stability of cytadherence-related proteins P140/P110 in Mycoplasma genitalium requires MG218 and unidentified factors.

Background: Tip-mediated cytadherence in Mycoplasma genitalium requires the structural and functional stability of the P140 adhesin, its operon-related protein P110, and the high molecular weight protein MG218 (190-kDa). Disruption mutants of mg218 unable to express MG218 exhibit both a non-cytadhering phenotype and P140/P110 instability, while disruption mutants that synthesize a truncated MG218 (160 kDa) retain the stability of P140/P110 and are >95% cytadhering. However, the origin of the MG218 truncated protein in these mutants is unclear.