Publications by authors named "Wanchan Chen"
Article Synopsis
- Epigenetic changes, particularly through circular RNAs (circRNAs), play a significant role in the development of cancer, with this study focusing on hsa_circ_0006470 and its interaction with miR-27b-3p in gastric cancer (GC).
- Researchers utilized techniques like qRT-PCR and dual-luciferase reporter assays to examine the expression and binding of hsa_circ_0006470, finding it to be highly expressed in GC cells.
- Silencing hsa_circ_0006470 led to reduced cancer cell proliferation, migration, and invasion while inducing autophagy, suggesting it promotes GC cell growth through the inhibition of miR-27b-3p and modulating
Background: Gastric cancer is the second leading cancer-related mortality worldwide and more effective treatment strategies are urgently needed to combat the disease. Using lipoteichoic acid (LTA) and miR-27b-3p agomir, we aimed to assess the efficacy of this combination of therapies in treating gastric cancer.
Methods: The RNA levels of miR-27b-3p, FOXO3, MET, KRAS, vascular endothelial growth factor C (VEGFC), TSC1, and P65 were analyzed by quantified-PCR (Q-PCR) and the cell viability of AGS cells was analyzed by MTT.
Article Synopsis
- - The study focuses on the long non-coding RNA FAM230B and its role in promoting gastric cancer (GC) by acting as a competing endogenous RNA (ceRNA) that interacts with miR-27a-5p.
- - Researchers conducted experiments showing that increased levels of FAM230B led to enhanced tumor cell growth, migration, and reduced apoptosis, while its knockdown had the opposite effects in gastric cancer cell lines.
- - Results suggest that FAM230B promotes GC by sponging miR-27a-5p, which normally inhibits cancer cell proliferation and invasion, ultimately increasing the expression of TOP2A, a gene associated with cancer progression.