Compression methods after femoral artery puncture: A protocol for systematic review and network meta-analysis.

Background: Vascular complications at the puncture site is a common complication after femoral artery puncture. It will not only affect the postoperative effect and patient comfort, but also may endanger the life of the patient. The effective compression hemostasis methods at the puncture site can improve the comfort of the patient, shorten the hospital stay, and reduce the burden on the medical staff.

Protective effect of propofol on hydrogen peroxide-induced human esophageal carcinoma via blocking the Wnt/β-catenin signaling pathway.

Objectives: To analyze the potential influences of propofol on the oxidative stress of HO-induced human esophageal squamous cell carcinoma (ESCC) Eca109 cell through mediating the Wnt/β-catenin signaling pathway.

Materials And Methods: Eca109 cells were classified into 5 groups: Control group, HO group, Propofol + HO group, Dkk1 (Dickkopf-1, Wnt/β-catenin pathway antagonist) + HO group, and Propofol + LiCl (Lithium chloride, Wnt/β-catenin pathway agonist) + HO group. Western blotting was performed to determine the protein expressions, flow cytometry to measure the content of ROS, immunofluorescence staining to detect the oxidative DNA damage, as well as MTT, AnnexinV-FITC/PI, Wound-healing, and Transwell assays to test the biological characteristics of Eca109 cells.