Metagenomics and metabolomics to investigate the effect of Amygdalus mongolica oil on intestinal microbiota and serum metabolites in rats.

Background: Renal fibrosis (RF) is an inevitable consequence of multiple manifestations of progressive chronic kidney diseases (CKDs). Mechanism of Amygdalus mongolica (Maxim.) in the treatment of RF needs further investigation.

Assessment of progression of pulmonary fibrosis based on metabonomics and analysis of intestinal microbiota.

The main purpose of this study was to explore the changes of biomarkers in different developmental stages of bleomycin-induced pulmonary fibrosis (PF) in rats comprehensive pathophysiology, UPLC-QTOF/MS metabonomic technology, and 16S rRNA gene sequencing of intestinal microbiota. The rats were randomly divided into normal control and 1-, 2- and 4-week model group. The rat model of PF was established by one-time intratracheal instillation of bleomycin.

Metabolomics reveal the mechanism for anti-renal fibrosis effects of an -butanol extract from .

To reveal the mechanism of anti-renal fibrosis effects of an -butanol extract from renal fibrosis was induced with unilateral ureteral obstruction (UUO) and then treated with an -butanol extract (BUT) from (Rosaceae). Sixty male Sprague-Dawley rats were randomly divided into the sham-operated, renal fibrosis (RF) model, benazepril hydrochloride-treated model (1.5 mg kg) and BUT-treated (1.

The intervention effect of oil on the metabolomics and intestinal flora in pulmonary fibrosis.

oil is rich in unsaturated fatty acids such as inoleic acid (47.11%) and oleic acid (23.81%).

Screening and identification of key microRNAs and regulatory pathways associated with the renal fibrosis process.

Renal fibrosis (RF) is a chronic and fatal disease related to the gradual deterioration of kidney function. MicroRNAs (miRNAs) play a key role in cellular functions and several of them related to the pathogenesis of RF have been identified, although the underlying mechanisms are unclear. In order to explore the miRNAs involved in RF progression, we established a model in rats by the unilateral ureteral ligation method.

A metabolomic approach to elucidate the inhibitory effects of baicalin in pulmonary fibrosis.

Context: Baicalin, a major flavonoid extracted from Georgi (Lamiaceae), has been shown to exert therapeutic effects on pulmonary fibrosis (PF).

Objective: To use serum metabolomics combined with biochemical and histopathological analyses to clarify anti-PF mechanisms of baicalin on metabolic pathways and the levels of potential biomarkers.

Materials And Methods: Forty male Sprague-Dawley rats were randomly divided into the control, PF model, prednisolone acetate-treated (4.

Metabolomic assessment of mechanisms underlying anti-renal fibrosis properties of petroleum ether extract from .

Context: The petroleum ether extract (PET) of (Maxim.) Ricker (Rosaceae) has an ameliorative effect on renal fibrosis (RF).

Objective: To evaluate the antifibrotic effects of seeds PET on RF by serum metabolomics, biochemical and histopathological analyses.

Protective effects of Amygdalus mongolica on rats with renal fibrosis based on serum metabolomics.

Ethnopharmacological Relevance: Renal fibrosis (RF) is a common outcome of various progressive chronic kidney diseases (CKDs) and, thus, seriously endangers human health. As the active ingredient of Amygdalus mongolica, amygdalin inhibits RF. Furthermore, our previous studies demonstrated that n-butanol extract (BUT) and petroleum ether extract (PET), which are effective components of A.

Isolation and identification of bovine nasopharyngeal mucosal epithelial cells and establishment of cell models of acute infection by foot-and-mouth disease virus.

Foot-and-mouth disease (FMD) commonly occurs via the respiratory tract, and bovine nasopharyngeal mucosal epithelial cells are the primary infection cells in cattle. The aim of the present study was to isolate and culture epithelial cells from the bovine nasopharyngeal mucosa in vitro using a mechanical separation method. The cells were expanded, established in continuous cell culture, and used for immunofluorescence cytochemistry and establishment of infection models.

[The analysis of the content of bile acids in ox-bile powder and goat gall powder of Mongolian medicine].

The present study was to detect and compare the content of bile acids in ox bile powder and goat gall powder of Mongolia medicine by UV. Cholic acid with sulphuric acid were heated and dehydrated, and they produced conjugated double bond. The conjugated bond showed the same absorption peak in the ultraviolet range.