[Isolation, culture and cryopreservation of cells derived from fetal appendages].

Objective: To investigate the optimal method for isolation, culture and cryopreservation of cells from fetal appendages, for the purpose of providing viable cells for tissue engineering, cell therapy and gene therapy.

Methods: Trypsin dispersion method was used to isolate cells from human umbilical cord and placenta. The tissues from umbilical cord and placenta were cryopreserved with dimethylsulfoxide (DMSO) in different concentrations.

[Preventing scarring in split-thickness skin donor sites with epidermal grafting].

Objective: Introducing a new technique for preventing the scar growthing in split thickness skin donor sites using the great sheets of epidermis covering.

Methods: The donor sites of split thickness skin were grafting with the great sheets of the epidermis, of the thickness about 0.07 approximately 0.