High water iodine levels are associated with the prevalence of subclinical hypothyroidism, thyroid nodules and goiter among pregnant women in Shandong, China.

Background: Both iodine deficiency and iodine excess can harm the thyroid glands during pregnancy. In areas without iodine fortification, the relationship between the water iodine concentration (WIC) and thyroid disease in pregnant women requires further investigation.

Objective: The aim of this study was to evaluate the relationship between water iodine levels and the prevalence of thyroid disease in pregnant women residing in areas with high water iodine levels without access to iodized salt.

Three new cyclopentanoid monoterpenes from the roots of .

Three new cyclopentanoid monoterpenes, neopiscrocins A-C (-), together with 14 known compounds (-), were isolated from the roots of . The structres of these compounds were elucidated on the basis of their spectroscopic data. All compounds were evaluated for cytotoxicity against six human tumor cell lines (PC9, PANC1, HCT-116, Hep-G2, BGC-823, and MCF-7), hepatoprotective activity and anti-inflammatory activity.

Two new iridoid glycosides from the whole plant of .

Two new iridoid glycosides, piasezkiiosides A () and B (), were isolated from aqueous extract of the whole plant of . Their structures were established from the spectroscopic data, chemical transformation, and X-ray diffraction analysis. Compound exhibited weak hepatoprotective activity against APAP-induced HepG2 cell damage.

Four new iridoid glycosides from the roots of .

Four new iridoid glycosides (-), rehmaglutosides L-O, were isolated from the air-dried roots of . Their structures were established from the spectroscopic data obtained and by chemical evidence. The known mellittoside () and ajugol () were also obtained in the current investigation, and the structure of mellittoside was unequivocally defined using X-ray diffraction data.

Seven new pentasaccharides from the roots of .

Seven new pentasaccharides (-), rehmaglupentasaccharides A-G, were isolated from the air-dried roots of . Their structures were established from the spectroscopic data obtained and by chemical evidence. The known verbascose () and stachyose () were also obtained in the current investigation, and the structure of stachyose was unequivocally defined using X-ray diffraction data.

Four ionones and ionone glycosides from the whole plant of .

Four new ionones and ionone glycosides (-) were isolated from the whole plant of Maxim. Their planar structures as well as absolute configuration were confirmed via spectroscopic analysis, ECD calculation, and X-ray crystallography. Compounds - were tested for their cytotoxicity against five human tumor cell lines and ability to inhibit LPS-activated NO production in the BV2 cell line.

The Effect of Posaconazole and Isavuconazole on the Pharmacokinetics of Erdafitinib in Beagle Dogs by UPLC-MS/MS.

A robust, quick, and reliable ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the quantification of erdafitinib in beagle dog plasma was developed and validated to evaluate the changes of posaconazole and isavuconazole on the pharmacokinetics of erdafitinib in beagle dogs, respectively. This experiment adopted a three-period self-control experimental design. In the first period (group A), erdafitinib was orally administered to six beagle dogs at a dose of 4 mg/kg.

Susceptibility and Resistance Mechanisms During Photodynamic Therapy of Melanoma.

Melanoma is the most aggressive malignant skin tumor and arises from melanocytes. The resistance of melanoma cells to various treatments results in rapid tumor growth and high mortality. As a local therapeutic modality, photodynamic therapy has been successfully applied for clinical treatment of skin diseases.

Automated on-line packed fiber solid phase extraction for determination of urinary catecholamines.

Despite the development of an off-line packed fiber solid phase extraction procedure (PFSPE) for urinary catecholamines, automation remains a challenge. Here, we propose an on-line PFSPE-HPLC procedure for automated sample processing and analysis of urinary catecholamines, with good recovery and precision, to avoid manual operation errors. The on-line PFSPE-HPLC procedure has been thoroughly optimized concerning the gradient, valve switch timing, the effects of complexing reagent and buffer solution, and the stability of the nanofibers.

Exploration of the appropriate recommended nutrient intake of iodine in healthy Chinese women: an iodine balance experiment.

There is a dearth of data on the iodine balance studies of Chinese population. In the present study, we aimed to explore the appropriate recommended nutrient intake (RNI) of iodine based on healthy Chinese women. A 4-week study was conducted in twenty-five Chinese euthyroid women.

High-throughput and selective solid-phase extraction of urinary catecholamines by crown ether-modified resin composite fiber.

In the present study, we developed a simple and high-throughput solid phase extraction (SPE) procedure for selective extraction of catecholamines (CAs) in urine samples. The SPE adsorbents were electrospun composite fibers functionalized with 4-carboxybenzo-18-crown-6 ether modified XAD resin and polystyrene, which were packed into 96-well columns and used for high-throughput selective extraction of CAs in healthy human urine samples. Moreover, the extraction efficiency of packed-fiber SPE (PFSPE) was examined by high performance liquid chromatography coupled with fluorescence detector.

Selective solid-phase extraction of catecholamines from plasma using nanofibers doped with crown ether and their quantitation by HPLC with electrochemical detection.

A simple and convenient preparation method has been developed for polymeric crown ethers containing the dibenzo-18-crown-6 subunit and electrospun composite nanofibers composed of polymeric crown ethers with polystyrene (PCE-PS). Furthermore, this composite nanofiber was used as a sorbent for selective extraction of plasma catecholamines, viz. dopamine (DA), norepinephrine (NE), and epinephrine (E).

[The change of NOS in pulmonary oxygen toxicity induced by different oxygen pressure].

Objective: Long time exhaled oxygen will induced oxygen toxicity. Some studies had found that different pathology may exised in normobaric and hyperbaric pulmonary oxygen toxicity, and nitric oxide synthase (NOS) may play a role. In this study, we discussed the change of NOS in normobaric and hyperbaric pulmonary oxygen toxicity.

Cyanidin-3-O-galactoside and blueberry extracts supplementation improves spatial memory and regulates hippocampal ERK expression in senescence-accelerated mice.

Objective: To investigate whether the antioxidation and the regulation on the Extracellular Regulated Protein Kinases (ERK) signaling pathway are involved in the protective effects of blueberry on central nervous system.

Methods: 30 Senescence-accelerated mice prone 8 (SAMP8) mice were divided into three groups and treated with normal diet, blueberry extracts (200 mg/kg•bw/day) and cyaniding-3-O-galactoside (Cy-3-GAL) (50 mg/kg•bw/day) from blueberry for 8 weeks. 10 SAMR1 mice were set as control group.

[Study on the iodine status of pregnant women and thyroid function of neonate in high iodine area].

Objective: To reveal the iodine status of pregnant women and its impact on thyroid function of neonates in high iodine area.

Methods: A total of 210 pregnant women were chosen from a hospital in Haixing county, Hebei province. Pregnant women's random urinary and neonates' cord blood were collected.

Thyroid dysfunction during late gestation is associated with excessive iodine intake in pregnant women.

Context: Adequate iodine intake during pregnancy is essential for both the synthesis of maternal thyroid hormones and the maintenance of normal fetal brain development. Scant evidence is available on the effects of excessive iodine intake during pregnancy.

Objective: The study assesses the relationship between iodine nutritional status and thyroid function of pregnant women with excessive iodine intake during late gestation.

[Investigation of thyroid function abnormalities in children in high water iodine areas of Hebei province].

Objective: To understand the level of thyroid function and the prevalence of the thyroid diseases of children in high water iodine areas.

Method: Two primary schools were selected by purposive sampling from Haixing county, Hebei province in April 2010. A total of 371 children were selected from six classes by cluster sampling in the schools.

[Construction of the dioxin bioassay method based on the clonal expressed aryl hydrocarbon receptor system].

Objective: To study the specific binding of the artificial clonal aryl hydrocarbon receptor translocator (ARNT) with the natural aryl hydrocarbon receptor (AhR) and the recolonization by polyclonal antibody. The dose-response relationship with tetrachlo-rodibenzo-dioxin (TCDD) was also studied to develop TCDD detection method and the binding degree related to dose response.

Methods: (1) The target genes including AhR-PAS, AhR-C and ARNT-PAS were amplified by RT-PCR by using the total RNA purified from the liver cells of C57BL/6J mice as templates to construct pGEX-5X1 recombinants.

[The effect of selenium on the expression of Fas/FasL in experimental autoimmune thyroiditis rats' thyroid with adequate iodine].

Objective: To study the effect of different selenium intake on the expression of apoptosis protein Fas/FasL in experimental autoimmune thyroiditis (EAT) rats' thyroid with adequate iodine.

Methods: Thirty-two female lewis rats were divided stochastically into 4 groups as C group, M group, Se(+) + M group, Se(-) + M group, respectively, and pretreated with feedstuffs containing different concentrations of selenium (Se(+) + M group 2 mg/kg, C and M group 0.20 mg/kg, Se(-) + M group 0.

[Role of mitogen-activated protein kinase pathway in chloracne].

Objective: To investigate the role of mitogen-activated protein kinase (MAPK) signal transduction pathway in chloracne.

Methods: Immunohistochemical technique was used to detect the expression of phosphorylated epidermal growth factor receptor (p-EGFR) and p-MAPK proteins in the epithelium of chloracne group and control group.

Results: p-EGFR and p-MAPK was found in all chloracne tissues, whereas no expression of p-EGFR and p-MAPK protein was found in control group.

[Effect of 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin on the expression of AhR and TGF-alpha].

Objective: To study HaCaT-keratinocyte cell lines, a chosen model of human epidermis in an attempt to analyze the mRNA expression of AhR and TGF-alpha induced by TCDD METHODS: Semi-quantitative reverse transcription PCR-technique was used for assaying the relative levels of AhR and TGF-alpha mRNA of HaCaT-cells during the proliferation period when the cells were cultured for 24 hours.

Results: Relative level of the AhR-transcripts (corrected with beta-actin) decreased with the elevated concentration of TCDD and the relevant coefficient between the proliferation rate and concentration was -0.548, and the differences among all groups were significant (F =4.

[Study on content of iodine in food in Tianjin market and iodine nutrition conditions among college students].

Objective: To know about content of iodine in foods sold in Tianjing markets presently, and the iodine nutrition conditions in college students. It was also aimed to probe the functions of the iodized salt complement with the dietary iodine intake, and whether the urine iodine could reflect dietary iodine intake.

Methods: 278 food samples in markets were collected by a randomly stratified sampling method, while the arsenic-cerium catalytic contact method was used to determine the content in food.

[Cloning, identification and tissue expression of novel genes differentially expressed in diabetic rats supplemented with zinc].

Objective: To clone novel gene fragments differentially expressed from diabetic rats supplemented with zinc and to detect their expression distribution in various tissues.

Methods: cDNA fragments from former research project were cloned, sequenced and BLASTn analysed. The RT-PCR of the two novel genes were made using the primers designed according to the sequence of cDNA to observe the expression changes in liver of various groups and their expression distributions in various tissues.