Tailor-made multiple sequence alignments using the PRALINE 2 alignment toolkit.

Summary: PRALINE 2 is a toolkit for custom multiple sequence alignment workflows. It can be used to incorporate sequence annotations, such as secondary structure or (DNA) motifs, into the alignment scoring, as well as to customize many other aspects of a progressive multiple alignment workflow.

Availability And Implementation: PRALINE 2 is implemented in Python and available as open source software on GitHub: https://github.