The proteomic and particle composition of human platelet lysate for cell therapy products.

Following health agencies warning, the use of animal origin supplements should be avoided in biological products proposed as therapy in humans. Platelet lysate and several other growth factors sources are alternatives to replace fetal calf serum, the current gold standard in clinical-grade cell culture. However, the platelet supplement's content lacks data due to different production methods.

Glyceraldehyde-3-phosphate dehydrogenase of the entomopathogenic fungus Metarhizium anisopliae: cell-surface localization and role in host adhesion.

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a classic glycolytic enzyme that plays important roles in various cellular processes. Here, we report the sequence and transcriptional analyses of a regulated gene (gpdh1) encoding GAPDH in the entomopathogenic fungus Metarhizium anisopliae, a well-characterized biocontrol agent of a wide range of arthropod pests. Transcript and protein analyses of the gpdh1 showed a carbohydrate-dependent expression pattern in response to different carbon sources.

Novel keratinase from Bacillus subtilis S14 exhibiting remarkable dehairing capabilities.

We report the isolation of a keratinolytic-producing Bacillus subtilis strain and the characterization of the exceptional dehairing properties of its subtilisin-like keratinase. This enzyme can be an alternative to sodium sulfide, the major pollutant from tanneries, and may completely replace it. Its unique nonactivity upon collagen enhances its industrial potential.