Design of a national patient-centred clinical pathway for sepsis in Sweden.

Background: The World Health Organization has adopted a resolution on sepsis and urged member states to develop national processes to improve sepsis care. In Sweden, sepsis was selected as one of the ten first diagnoses to be addressed, when the Swedish government in 2019 allocated funds for patient-centred clinical pathways in healthcare. A national multidisciplinary working group, including a patient representative, was appointed to develop the patient-centred clinical pathway for sepsis.

Detailed Analysis of the Characteristics of Sample Volume in Blood Culture Bottles.

Blood volume is the most important variable for the detection of microorganisms in blood cultures (BCs). Most standards recommend 40 to 60 ml blood, collected in several BC bottles filled up to 10 ml. We measured blood volume in individual BC bottles and analyzed the associations of hospital, bottle type, day of the week, daily sampling time, and age and sex of the patient with sampling volume and BC result.

An animal model to study health effects during continuous low-dose exposure to the nerve agent VX.

In the present study, we have developed an animal model to study long-term health effects of continuous exposure of toxic chemical agents, in awake, freely moving rats. The aim was to evaluate the effect of low-dose exposure of the nerve agent VX, and to find specific biomarkers for intoxication. To exclude the influence of stress, we used an implanted radio-telemetric device for online registration of physiological parameters, and an osmotic pump, implanted subcutaneously, for continuous exposure of the toxic agent.

Knee-heel length measurements in preterm infants: evaluation of a simple electronically equipped instrument.

Aim: To compare and evaluate a mini-knemometer with a simple and inexpensive electronic caliper with regard to precision, handling error (technical error) and estimation of growth velocity.

Methods: Thirty-five prematurely born infants, with a median gestational age of 29 (range 24-33) wk and a median birthweight of 960 (range 480-2,480) g, were measured on 409 different occasions with both instruments. On each occasion, five independent readings were made.

Two point mutations convert a catalytically inactive carbonic anhydrase-related protein (CARP) to an active enzyme.

A murine carbonic anhydrase-related protein (CARP) has been expressed in Escherichia coli and purified to near homogeneity. The polypeptide chain consists of 290 amino acid residues and has a calculated molecular mass of 32,950 Da. By introducing two mutations, Arg117 --> His and Glu115 --> Gln, we created a metal-binding center homologous to that in the carbonic anhydrases from the animal kingdom.

Histidine 64 is not required for high CO2 hydration activity of human carbonic anhydrase II.

To test the hypothesis that histidine 64 in carbonic anhydrase II has a crucial role as a 'proton shuttle group' during catalysis of CO2-HCO3- interconversion, this residue was replaced by lysine, glutamine, glutamic acid and alanine by site-directed mutagenesis. All these variants turned out to have high CO2 hydration activities. The kcat values at pH 8.

The effect of temporal parameters on subjective sensations evoked by electrical tooth stimulation.

The effect of stimulus duration and frequency on subjective sensations evoked by electrical tooth stimulation was studied in 12 subjects. The sensory responses were classified using 5 equi-sensation categories (perception threshold, prepain, pain threshold, moderate pain, intense pain). Both continuously increasing and randomised stimuli were applied.

The diagnostic value of the hemoccult as a screening test in patients taking anticoagulants.

The value of a 3-day Hemoccult II test in the diagnosis of colorectal disease in patients receiving anticoagulant therapy was studied. The influence of rehydration and nonrehydration of the slide was investigated, as well as the reproducibility of positive tests. Eight hundred forty-nine patients on anticoagulant therapy performed three Hemoccult II tests each.

Metrizamide and iothalamate meglumine in peripheral angiography in arterial insufficiency in the legs.

In 41 patients peripheral angiography was performed with the non-ionic contrast medium metrizamide (Amipaque) and the ionic iothalamate meglumine (Conray 60). Amipaque was found to give less subjective adverse effects and less changes in blood pressure.