Publications by authors named "Waldemar Silva Costa"
Article Synopsis
- The study aimed to assess the impact of a cafeteria diet on the glomerular structure in male Wistar rats using both quantitative and qualitative methods.
- Male rats were divided into control and cafeteria diet groups, followed until 5 months of age for analysis of blood, kidneys, and fat deposits.
- The results showed that the cafeteria diet led to significant glucose intolerance and changes in fat accumulation, while altering glomerular basement membrane thickness, highlighting the need for further research on diet effects on kidney function.
Purpose: To investigate the effects of chronic stress in the prostate of prepubertal and adult rats.
Methods: Thirty-two male rats were assigned into four groups depending on the type of treatment (control or stressed) and the age at which stress was initiated (prepubertal or adult). Restraint stress stimuli were applied for six weeks.
Purpose: To compare the penile histoarchitecture of rats euthanized with isoflurane or with ketamine and xylazine.
Methods: Fourteen male rats were divided into two groups: ISO, with animals euthanized with isoflurane; and K+X, with animals euthanized with ketamine (150 mg/kg) associated with xylazine (15 mg/kg). Immediately after the death, the penises were dissected, fixed in a 4% buffered formalin solution, and processed for histomorphometric analysis.
Purpose: To quantify and compare the expression of stromal elements in prostate adenocarcinoma of different Gleason scores with non-tumor area (control).
Methods: We obtained 132 specimens from samples of prostate peripheral and transition zone. We analyzed the following elements of the extracellular matrix: collagen fibers, elastic system, smooth muscle fibers and blood vessels.
The present study aimed to evaluate the effects of chronic stress from the prepubertal to the adult stages or during adulthood on penile morphology in rats. The animals were immobilized in a cylinder for 2 h daily for a total of six weeks to simulate stress. Ten rats were exposed to stress stimulus beginning from the prepubertal age, while nine rats were exposed to stress stimulus only during adulthood.
View Article and Find Full Text PDFBackground/aims: Stress had been related to physiological and morphological alterations in different organs, however kidney morphology and function after stress conditions are not well-known. To evaluate the effects of chronic stress on renal morphology in prepubertal and adult rats, and to investigate if the effects of induced stress remain stable or worsen following removal of the stress stimuli.
Methods: Forty prepubertal rats (4 weeks old) and thirty six adult rats (10 weeks old) were used.
Purpose:: To investigate the glomerular number after different warm ischemia times.
Methods:: Thirty two pigs were assigned into four groups. Three groups (G10, G20, and G30) were treated with 10, 20, and 30 minutes of left renal warm ischemia.
Purpose: To compare, with stereological methods, the glomerular loss in kidneys submitted to radiofrequency ablation (RFA) or warm ischemia.
Materials And Methods: Twenty-six male pigs were divided into three groups. Eight animals were allocated in the sham group, which was submitted to laparoscopic dissection of the left renal hilum, without renal ischemia.
Purpose:: To evaluate the glomerular loss after arteriovenous or arterial warm ischemia in a swine model.
Methods:: Twenty four pigs were divided into Group Sham (submitted to all surgical steps except the renal ischemia), Group AV (submitted to 30 minutes of warm ischemia by arteriovenous clamping of left kidney vessels), and Group A (submitted to 30 minutes of ischemia by arterial clamping). Right kidneys were used as controls.
Purpose:: To investigate the protective effect of L-arginine on the prostate (nonneoplasic) of rats with radiation-induced injury.
Methods:: Twenty-nine Wistar rats, male adult, allocated into three groups: Control group (C) was not exposed to irradiation (n=10); Radiated group (R) had undergone pelvic irradiation (n=10); Supplemented and radiated group (R+S) had undergone pelvic irradiation plus L-arginine supplementation (n=9). The animals were observed for signs of toxicity.
The use of morphometrical tools in biomedical research permits the accurate comparison of specimens subjected to different conditions, and the surface density of structures is commonly used for this purpose. The traditional point-counting method is reliable but time-consuming, with computer-aided methods being proposed as an alternative. The aim of this study was to compare the surface density data of penile corpus cavernosum trabecular smooth muscle in different groups of rats, measured by two observers using the point-counting or color-based segmentation method.
View Article and Find Full Text PDFPurpose: To evaluate the penile morphological modifications of pubertal and adult rats chronically treated with supra-physiological doses of anabolic androgenic steroids.
Methods: Forty-eight male Wistar rats were distributed into four groups: two control groups, 105- and 65-day-old (C105 and C65, respectively) injected with peanut oil (vehicle); and two treated groups, 105- and 65-day-old (T105 and T65, respectively) injected with nandrolone decanoate at a dose of 10 mg Kg-1 of body weight. The rats were injected once a week for eight weeks.
Purpose: To investigate the structural and functional changes induced by corticosterone (CORT) in the ventral prostrate (VP) of rats in order to study chronic stress effects in the prepubertal phase.
Methods: Wistar rats received daily saline or CORT injections during the pubertal period from the 5th to 25th day of postnatal life. The animals were distributed into four groups: 1 - Control (n=5); 2 - Control 99mTc-P (n=5); 3 - Treated with CORT (n=14); 4 - Treated with CORT and 99mTc-P (n=10).
Article Synopsis
- The study aimed to analyze the endopelvic fascia structure in patients with different prostate weights, specifically those with benign prostatic hyperplasia (BPH) and prostate adenocarcinoma (PAC).
- Samples were taken from patients during surgery, and the analysis involved assessing elastic fibers, collagen, and smooth muscle using advanced imaging programs and statistical methods.
- Results showed significant differences in smooth muscle cell quantity among the groups, especially highlighting that heavier prostates (BPH) had lower smooth muscle content compared to lighter prostates (PAC) and younger controls, which may impact surgical evaluations for radical prostatectomy.
Article Synopsis
- - The study aimed to quantitatively analyze pelvic ureter tissues in adults with chronic dilation using stereological methods, focusing on samples from patients who underwent ureteral reimplantation surgery.
- - Researchers compared tissue samples from 6 patients with dilated ureters to a control group of 7 kidney transplant donors, assessing the volumetric density of collagen, elastic fibers, and smooth muscle fibers.
- - Results indicated no significant difference in collagen volume between the two groups, but the dilated ureters had significantly higher volumes of both elastic and smooth muscle fibers compared to controls.
Aim: To evaluate the influence of dietary lipid quality on the body mass, carbohydrate metabolism and morphology of the rat ventral prostate.
Materials And Methods: Wistar rats were divided into four groups: SC (standard chow), HF-S (high-fat diet rich in saturated fatty acids), HF-P (high-fat diet rich in polyunsaturated fatty acids) and HF-SP (high-fat diet rich in saturated and polyunsaturated fatty acids). We analyzed body mass, fat mass deposits, plasma blood, insulin resistance and the ventral prostate structure.
Purpose: Many adverse effects have been associated with abuse of anabolic-androgenic steroids (AAS), including disorders of the urogenital tract. The objective of this study is to analyze the morphological modifications in the prostate ventral lobe of pubertal and adult rats chronically treated with AAS, using morphometric methods.
Materials And Methods: We studied 39 male Wistar rats weighing between 400 g and 550 g.
Objective: To assess the morphological changes of penile vascular structures and the corpus cavernosum area in alloxan-induced diabetic rabbits.
Materials And Methods: Twenty male rabbits (2 months old) were divided into two groups with 10 rabbits each, the control group (CG) and the diabetic group (DG). The animals from DG received an intravenous injection of alloxan (100mg/kg) to induce the diabetes.
Purpose: To investigate the seminiferous tubule histological morphology after an 8 mmHg pneumoperitoneum in the rat model.
Methods: Fourteen rats were divided into two groups: a Sham group submitted to anesthesia and a pneumoperitoneum (Pp) group submitted to abdominal insufflation at 8 mmHg during three hours, followed by desuflation. All rats were killed after six weeks, testicles were collected and evaluated for the tubule diameter, germinative epithelium height and Johnsen´s score.
Article Synopsis
- The study aimed to explore how elastic fiber content in different tissues of the rabbit penis changes as the rabbits age.
- Researchers sacrificed groups of rabbits at various ages (30, 120, 240, and 730 days) and analyzed tissue samples to determine elastic fiber volume with specific staining techniques.
- Results showed a significant increase in elastic fiber content in older rabbits, especially in the corpus spongiosum, but this trend plateaued or decreased at two years old, suggesting possible age-related changes in penile tissue properties.
Objectives: To evaluate histological alterations in prepuce of patients with phimosis submitted to topic treatment with betamethasone in association with hyaluronidase.
Materials And Methods: We studied sixty patients (mean age 4.5), presenting true phimosis and treated with a topical treatment with betamethasone cream (0.
Aims: Foreskin development begins at twelfth gestational week through a circular invagination of the ectoderm in the glandular periphery that grows ventrally and totally involves the glans around the twentieth gestational week. Studies of foreskin formation chronology and its histological constituents in human fetuses are rare. The objective of this study is to analyze foreskin development during the second trimester of the human fetal period.
View Article and Find Full Text PDFThis paper verifies the morphological changes induced by immobilization stress on the kidney of rats by using stereological methods. Fifteen 4-week-old Wistar male rats were randomly assigned to control (n = 7) and stressed (n = 8) groups. Stress stimuli were performed over 5 weeks by immobilization of the rats for 2 h daily in a rigid opaque plastic cylinder that restrained their movements.
View Article and Find Full Text PDFObjective: Patients who have had pelvic radiotherapy as part of their cancer therapy may develop subsequent urinary bladder effects such as hyperactive bladder, incontinence, and dysuria. Therefore, the goal of this study was to evaluate whether glutamine supplementation could prevent collagen expression damage in healthy urinary bladder caused by radiotherapy.
Methods: Fifteen adult Wistar rats were separated into a control group that received food and water ad libitum (C group), an irradiated group that received a single pelvic radiation dose of 1164 cGy (I group), and an irradiated group supplemented with l-glutamine every day during the entire experimental period (0.
Objectives: To access the kidney healing after laparoscopic partial nephrectomy without closing of the collecting system in pigs.
Methods: Fourteen pigs underwent left partial laparoscopic nephrectomy, with removal of 25% of the kidney length at caudal pole (n = 7) or at cranial pole (n = 7). Briefly, the surgical technique involved a transperitoneal laparoscopic access, en bloc vascular clamping of renal vessels, tissue excision with cold scissor and monopolar energy hemostasis of only the parenchyma, leaving the collecting system opened, with no insertion of a double-J catheter.