Peptidomic Analysis and Antimicrobial Activity of Serum Peptide from Clone BPM24.

Background: Hevea brasiliensis is severely affected by the fungal disease caused by spp. Significant loss of rubber yield is widespread and extensive use of chemical fungicides has resulted in health and environmental problems.

Objective: This work aims to extract and identify the latex serum peptides from a disease tolerant clone of , and study the inhibitory efficacy against pathogenic bacteria and fungi.

His in the pore-lining α4 of the Bacillus thuringiensis Cry4Aa δ-endotoxin is crucial for structural arrangements of the α4-α5 transmembrane hairpin and hence biotoxicity.

One proposed toxic mechanism of Bacillus thuringiensis Cry δ-endotoxins involves pore formation in target membranes by the α4-α5 transmembrane hairpin constituting their pore-forming domain. Here, nine selected charged and uncharged polar residues in the pore-lining α4 of the Cry4Aa mosquito-active toxin were substituted with Ala. All mutant toxins, i.