seed proteins effect on snake venom enzymes with antioxidant and antibacterial activities.

Objective: This study assessed the inhibitory potential of proteins extracted from seeds on snake venom toxic enzymes along with their potential antioxidant and antibacterial activities.

Methods: Crude proteins were extracted using common biological buffers (20 mM acetate, 20 mM phosphate and 20 mM Tris) at a ratio of 1:5 followed by 80 % ammonium sulfate precipitation, dialysis, and lyophilization. Then the lyophilized extracts were resolved on 15 % sodium dodecyl sulfate polyacrylamide electrophoresis (SDS-PAGE) gels.