Molecular characterization of genes responsible for biofilm formation in isolated from mastitic cows.

Background And Aim: Mastitis is considered a significant disease of lactating animals. There are new attitudes for recognizing genes responsible for causing this disease to overcome and change the manipulation of this problem. This study aimed to isolate and identify strains from mastitic bovine animals and detect some specific biofilm-forming genes (, , and biofilm-associated protein [] genes , and ).

Genotyping and phylogenetic analysis of canine parvovirus circulating in Egypt.

Aim: This study aimed to detect and characterize current genotypes of canine parvovirus (CPV) in Egypt during 2018.

Materials And Methods: A total of 50 fecal swabs were collected from clinically infected domestic dogs of 2-5 months of age, suspected to suffer from CPV infection, from Cairo and Giza Governorates. The samples were subjected to qualitative antigen detection using the rapid test, followed by isolation on Madin-Darby Canine Kidney (MDCK) cells, molecular characterization with partial amplification of VP2 gene using polymerase chain reaction (PCR), followed by sequencing and phylogenetic analysis.