Publications by authors named "Wael Eter"
Article Synopsis
- - Purpose: The study aims to enhance understanding of diabetes by monitoring β-cell mass and function using a combined PET/MRI protocol, which allows for real-time assessment without needing to pre-label isolated cells.
- - Methods: Islets were transplanted into mice, and their mass and function were tracked using PET and MRI at various intervals post-transplant, with validations through additional imaging techniques to ensure accuracy.
- - Results: The imaging approach successfully monitored the growth and function of transplanted islets, showing a promising correlation between in vivo imaging data and validation methods, while highlighting the need for further research to optimize measurement of β-cell function.
Article Synopsis
- GLP-1R imaging using radiolabeled exendin is an effective method for measuring β-cell mass (BCM) in living organisms.
- High blood glucose levels lead to reduced exendin uptake in both mouse and human islets, correlating with decreased GLP-1R expression.
- Normalizing blood glucose levels can restore exendin uptake and GLP-1R expression, highlighting the importance of stable glycemic control before starting GLP-1R treatments or BCM assessments.
Pancreatic islet transplantation is a promising therapy for patients with type 1 diabetes. However, the duration of long-term graft survival is limited due to inflammatory as well as non-inflammatory processes and routine clinical tests are not suitable to monitor islet survival. In-exendin-SPECT (single photon emission computed tomography) is a promising method to non-invasively image islets after transplantation and has the potential to help improve the clinical outcome.
View Article and Find Full Text PDFSingle Photon Emission Computed Tomography (SPECT) has become a promising experimental approach to monitor changes in β-cell mass (BCM) during diabetes progression. SPECT imaging of pancreatic islets is most commonly cross-validated by stereological analysis of histological pancreatic sections after insulin staining. Typically, stereological methods do not accurately determine the total β-cell volume, which is inconvenient when correlating total pancreatic tracer uptake with BCM.
View Article and Find Full Text PDFIslet transplantation is a novel promising strategy to cure type 1 diabetes. However, the long-term outcome is still poor, because both function and survival of the transplant decline over-time. Non-invasive imaging methods have the potential to enable monitoring of islet survival after transplantation and the effects of immunosuppressive drugs on transplantation outcome.
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