PCR combined with lateral flow dipstick assay (PCR-LFD) for a rapid diagnosis of melioidosis.

Background: Melioidosis is an infectious disease caused by Burkholderia pseudomallei. Septicemic melioidosis patients have a high mortality rate within 48 hours.

Objective: To develop a polymerase chain reaction (PCR) combined with a lateral flow dipstick (LFD) assay for detection of B.