Rheology of protein-stabilised emulsion gels envisioned as composite networks. 2 - Framework for the study of emulsion gels.

Hypothesis: The aggregation of protein-stabilised emulsions leads to the formation of emulsion gels. These soft solids may be envisioned as droplet-filled matrices. Here however, it is assumed that protein-coated sub-micron droplets contribute to the network formation in a similar way to proteins.

Rheology of protein-stabilised emulsion gels envisioned as composite networks 1- Comparison of pure droplet gels and protein gels.

Hypothesis: Protein-stabilised emulsion gels can be studied in the theoretical framework of colloidal gels, because both protein assemblies and droplets may be considered as soft colloids. These particles differ in their nature, size and softness, and these differences may have an influence on the rheological properties of the gels they form.

Experiments: Pure gels made of milk proteins (sodium caseinate), or of sub-micron protein-stabilised droplets, were prepared by slow acidification of suspensions at various concentrations.

DNA-Coated Functional Oil Droplets.

Many industrial soft materials include oil-in-water (O/W) emulsions at the core of their formulations. By using tuneable interface stabilizing agents, such emulsions can self-assemble into complex structures. DNA has been used for decades as a thermoresponsive, highly specific binding agent between hard and, recently, soft colloids.

Thermal Hysteresis and Seeding of Twisted Fibers Formed by Achiral Discotic Particles.

In this paper, molecular dynamics simulations of simple disc-shaped particles are used to investigate the free self-assembly of defect-free fibers. Depending on the choice of particle shape and interaction strength, the formed fibers are reproducibly either straight or, for reasons of packing efficiency, spontaneously chiral. As they grow radially, increasing stresses cause chiral fibers to untwist either continuously or via morphological rearrangement.

Self-assembly of small peptide amphiphiles, the structures formed and their applications. (A foods and home and personal care perspective).

In this opinion piece, some specific challenges in the field of peptide self-assembly and gel formation are discussed. One major hurdle to finding functional small peptides is that there are a huge number of compounds to explore, which increases exponentially with the peptide size. This in itself creates a barrier to the discovery and application of materials, both through the difficulty of finding the peptides, and because protecting inventions also becomes more difficult.

Magnetically aligned supramolecular hydrogels.

The magnetic-field-induced alignment of the fibrillar structures present in an aqueous solution of a dipeptide gelator, and the subsequent retention of this alignment upon transformation to a hydrogel upon the addition of CaCl2 or upon a reduction in solution pH is reported. Utilising the switchable nature of the magnetic field coupled with the slow diffusion of CaCl2 , it is possible to precisely control the extent of anisotropy across a hydrogel, something that is generally very difficult to do using alternative methods. The approach is readily extended to other compounds that form viscous solutions at high pH.

A microrheological study of hydrogel kinetics and micro-heterogeneity.

The real-time dynamic heterogeneity of the gelation process of the amino acid derivative Fmoc-tyrosine (Fmoc-Y) is studied using particle tracking microrheology. To trigger gelation, glucono-δ-lactone (GdL) is added, which gradually lowers the p H over several hours. The onset of self-assembly in the system is signified by a sharp drop in the mean-squared displacement of embedded particles, a phenomenon that is found to correlate with the p H of the system reaching the pK(a) of Fmoc-Y.

Microrheology and microstructure of Fmoc-derivative hydrogels.

The viscoelasticity of hydrogel networks formed from the low-molecular-weight hydrogelator Fmoc-tyrosine (Fmoc-Y) is probed using particle-tracking microrheology. Gelation is initiated by adding glucono-δ-lactone (GdL), which gradually lowers the pH with time, allowing the dynamic properties of gelation to be examined. Consecutive plots of probe particle mean square displacement (MSD) versus lag time τ are shown to be superimposable, demonstrating the formation of a self-similar hydrogel network through a percolation transition.

Hierarchical self-assembly of chiral fibres from achiral particles.

We investigate, by molecular dynamics simulation, the behaviour of discotic particles in a solvent of Lennard-Jones spheres. When chromonic disc-sphere interactions are imposed on these systems, three regimes of self-assembly are observed. At moderate temperatures, numerous short threads of discs develop, but these threads remain isolated from one another.

The influence of the kinetics of self-assembly on the properties of dipeptide hydrogels.

We discuss the effect of the kinetics of pH change on the mechanical properties of dipeptide hydrogels. Data from other peptide-based low molecular weight gelator (LMWG) systems suggest that the rheological properties are often highly dependent on the assembly rate. To examine kinetics here, we have used the hydrolysis of glucono-8-lactone (GdL).

Mixed biopolymer aqueous solutions--phase behaviour and rheology.

Mixed biopolymer solutions are found in many food systems and household products, and are also employed in industrial processes such as bio-separation and purification. They display a rich phase behaviour, ranging from association and precipitation to the more common segregative phase separation into two liquid phases. Understanding the underlying physics of their phase behaviour and of the rheology-morphology relationships of the resulting phases is a topic of interest and importance in terms of being able to reliably design and produce products containing mixed biopolymer solutions and predicting their behaviour.

Emulsification mechanism and storage instabilities of hydrocarbon-in-water sub-micron emulsions stabilised with Tweens (20 and 80), Brij 96v and sucrose monoesters.

The influence of both the nature of the surfactant and surfactant concentration on the processes of droplet break-up and coalescence in the formation of decane-in-water nano-emulsions in a high-pressure homogenizer was investigated. Emulsions were produced using a Christison Scientific M110-S microfluidiser with an impinging jet high-shear chamber. For all six surfactants studied (Tween 20, Tween 80, Brij 96v, sucrose monolaurate, sucrose monomyristate and sucrose monopalmate), the droplet size decreased with increasing surfactant concentration reaching a limiting droplet size at a surfactant concentration of 15 mM.

Controlled release from modified amino acid hydrogels governed by molecular size or network dynamics.

Hydrogels can be prepared using the commercially available Fmoc-phenylalanine or Fmoc-tyrosine as the gelator. Gelation is triggered by careful adjustment of the pH of the solution using glucono-delta-lactone (GdL). Model dyes have been entrapped in the hydrogels, and the release of the dyes from the hydrogels has been monitored.

Rheology of gelling and yielding soft matter systems.

Yielding and gelling soft matter materials are ubiquitous throughout biological and geological systems, the most commonly encountered examples being in food and other household products. In this Highlight, we attempt to summarise the rheological properties that are characteristic of structured soft matter systems, including their universal flow behaviour and viscoelastic response as well as appropriate methods of characterisation. We also discuss how the mechanical response depends on the materials' microstructure.

Interfacial tension in aqueous biopolymer-surfactant mixtures.

The current study offers a first insight into the interfacial properties of pullulan-sodium dodecyl sulphate (SDS) aqueous two-phase systems (ATPS) in the presence of sodium chloride (NaCl). The effect of composition on the interfacial tension (sigma) in these ATPS was investigated over a wide range of pullulan, SDS and NaCl concentrations. An increase in the interfacial tension was observed with increasing pullulan and SDS concentrations and a small increase was also observed as the NaCl concentration was increased.

Direct measurement of the effective charge in nonpolar suspensions by optical tracking of single particles.

The authors develop an ultrasensitive method for the measurement of the charge carried by a colloidal particle in a nonpolar suspension. The technique uses the phenomenon of the resonance of a particle held in an optical tweezer trap and driven by a sinusoidal electric field. The trapped particle forms a strongly damped harmonic oscillator whose fluctuations are a function of gamma, the ratio of the root-mean-square average of the electric and thermal forces on the particle.

Synergistic interaction in emulsions stabilized by a mixture of silica nanoparticles and cationic surfactant.

Using a range of complementary experiments, a detailed investigation into the behavior of dodecane-water emulsions stabilized by a mixture of silica nanoparticles and pure cationic surfactant has been made. Both emulsifiers prefer to stabilize o/w emulsions. At high pH, particles are ineffective emulsifiers, whereas surfactant-stabilized emulsions become increasingly stable to coalescence with concentration.

Characteristics and risks of drivers with low annual distance driven.

Objectives: It has been noted by several authors that risk (defined only in terms of total expected numbers of crash involvements per total distance driven) paints a misleading picture of crash liability, particularly for the young and the old, as their high risk is associated with risky driving patterns typical of people who drive low annual kms. This article sets out to analyze these driving patterns of low-km drivers and to evaluate the risk of these patterns. As licensing programs tend to focus on young and old drivers, who tend to drive lower annual distances, income and employment data are also analyzed for low-km drivers.

Interfacial tension in phase-separated gelatin/dextran aqueous mixtures.

The effect of solute concentrations on interfacial tension was investigated in phase-separated mixtures of dextran and gelatin over a range of concentrations that covered different tie-lines and different positions on one tie-line. The investigations were carried out using equilibrated gelatin-rich and dextran-rich phases in a computer-controlled Couette device at 40 degrees C (above the gelation point of gelatin) and interfacial tensions were measured using the retracting drop method. The results show that the interfacial tension can be related to the length of the tie-line or to the difference in the concentration of dextran (or gelatin) in the separated phases.

The contribution of alcohol to night time crash risk and other risks of night driving.

Many studies show that driving at night is more risky in terms of crash involvements per distance travelled than driving during the day. The reasons for this include the more prevalent use of alcohol by drivers at night, the effects of fatigue on the driving task and the risk associated with reduced visibility. Although the consumption of alcohol prior to driving occurs most commonly at night, drink-driving is not inherently a night time risk factor.

Association between older driver characteristics, on-road driving test performance, and crash liability.

From May 1999, a new system for licensing older drivers was introduced in New Zealand. It included a practical on-road driving test with expanded scope, to be completed every two years from the time the driver turns 80. The relationship between crashes and test performance needed to be studied to inform the debate regarding the testing system.

Older driver crash rates in relation to type and quantity of travel.

It is a well-established phenomenon that, notwithstanding their overall good crash record, older drivers have a higher than average rate of involvement in injury crashes when the rate is calculated by dividing crash numbers by distance driven. It has been hypothesised that at least some of this higher crash rate is an artefact of the different nature of driving undertaken by many older drivers. For example, driving in congested urban environments provides more opportunities for collisions than driving the same distance on a motorway.

The influence of alcohol, age and number of passengers on the night-time risk of driver fatal injury in New Zealand.

Breath alcohol measurements and other data collected at randomly selected roadside sites were combined with data on fatally injured drivers in crashes occurring on the same weekdays and times (Friday and Saturday nights) at locations matched by the size of the nearest town. A logistic model was fitted to these data for the years 1995-2000 to estimate the effects of alcohol, driver's age and the influence of passengers carried on the risk of driver fatal injury in New Zealand. The estimated risks increased steeply with increasing blood alcohol concentration (BAC), closely following an exponential curve at levels below about 200mg/dl (i.

Further results from a trial comparing a hidden speed camera programme with visible camera operation.

As described in a previous paper [Accident Anal. Prev., 33 (2001) 277], the hidden camera programme was found to be associated with significant net falls in speeds, crashes and casualties both in 'speed camera areas' (specific signed sites to which camera operation is restricted) and on 100 km/h speed limit roads generally.

Dynamic experimentation on the confocal laser scanning microscope: application to soft-solid, composite food materials.

Confocal laser scanning microscopy (CLSM) is used to follow the dynamic structural evolution of several phase-separated mixed biopolymer gel composites. Two protein/polysaccharide mixed gel systems were examined: gelatin/maltodextrin and gelatin/agarose. These materials exhibit 'emulsion-like' structures, with included spherical particles of one phase (i.