Biomarkers of epithelial-mesenchymal transition in localized, surgically treated clear-cell renal cell carcinoma.

Introduction: It has been suggested that the metastatic potential of neoplastic cells can be predicted on the basis of their biological features, including expression of proteins involved in the epithelial to mesenchymal transition (EMT). Therefore, the purpose of this work was to (1) evaluate the expression of EMT markers: ZEB2, vimentin, N-cadherin, TWIST, PTEN, survivin, E-cadherin, Ki-67 and GLUT-1, (2) assess mutation status of two genes: PIK3CA and KRAS, and (3) investigate the potential relationships between the studied biomarkers and clinicopathological factors in clear-cell renal cell carcinoma (ccRCC).

Material And Methods: Tumor tissue samples (embedded in paraffin blocks) from 159 patients undergoing radical nephrectomy were analyzed.

MCPIP1 Downregulation in Clear Cell Renal Cell Carcinoma Promotes Vascularization and Metastatic Progression.

Clear cell renal cell carcinoma (ccRCC) is the most common type of kidney cancer and it forms highly vascularized tumors. The monocyte endoribonuclease MCPIP1 negatively regulates inflammation by degrading mRNA encoding proinflammatory cytokines, such as IL6, IL1, and IL12. MCPIP1 is also a negative regulator of NFκB and AP1 activity and it influences a broad range of miRNA activities.

MCPIP1 contributes to clear cell renal cell carcinomas development.

Monocyte Chemoattractant protein-induced protein 1 (MCPIP1), also known as Regnase-1, is encoded by the ZC3H12a gene, and it mediates inflammatory processes by regulating the stability of transcripts coding for proinflammatory cytokines and controlling activity of transcription factors, such as NF-κB and AP1. We found that MCPIP1 transcript and protein levels are strongly downregulated in clear cell renal cell carcinoma (ccRCC) samples, which were derived from patients surgically treated for renal cancer compared to surrounded normal tissues. Using Caki-1 cells as a model, we analyzed the role of MCPIP1 in cancer development.

Histopathological assessment of residual retroperitoneal mass removed in patients after chemotherapy for non-seminomatous germ cell tumours of the testis.

Between 1990 and 1999, 182 men were treated for non-seminomatous germ cell testicular tumours. In 24 of them after chemotherapy a residual retroperitoneal mass was removed. In 14 of them additional immunohistochemical (IHC) examinations using antibodies against cytokeratins, vimentin, PLAP, CD30, AFP, βhCG, p53, and MIB-1 were performed.

Variants of SCARB1 and VDR Involved in Complex Genetic Interactions May Be Implicated in the Genetic Susceptibility to Clear Cell Renal Cell Carcinoma.

The current data are still inconclusive in terms of a genetic component involved in the susceptibility to renal cell carcinoma. Our aim was to evaluate 40 selected candidate polymorphisms for potential association with clear cell renal cell carcinoma (ccRCC) based on independent group of 167 patients and 200 healthy controls. The obtained data were searched for independent effects of particular polymorphisms as well as haplotypes and genetic interactions.

Monocyte chemoattractant protein-induced protein 1 impairs adipogenesis in 3T3-L1 cells.

Monocyte chemoattractant protein-induced protein 1 (MCPIP1) encoded by the ZC3H12a gene (also known as Regnase-1) is involved in the regulation of degradation of mRNA of inflammatory modulators and for processing of pre-miRNA. These functions depend on the presence of the PIN domain. Moreover, MCPIP1 was described as a negative regulator of NF-κB and AP-1 signaling pathways although mechanisms underlying such activity remain unknown.

Differences in the expression of telomerase and prostate-specific membrane antigen in non-advanced prostatic cancer.

Early recognition of prostate cancer (PC) based on biological markers could be helpful in identification of differences in benign and malignant lesions and facilitate further precise indication for more aggressive treatment. Therefore, the aim of our study was to assess expression of hTERT (human telomerase reverse transcriptase, a catalytic subunit of telomerase) and prostate-specific membrane antigen (PSMA), both considered to be markers of tumor aggressiveness. 140 low advanced PC specimens from patients who underwent radical prostatectomy were studied.

Microvessel density and expression of vascular endothelial growth factor in clinically localized prostate cancer.

Identifying biological differences between benign lesions and malignant prostatic cancer (PC) may facilitate precise indication for more aggressive post-operative treatment. Therefore, we examined immunohistochemically histological specimens from 140 PC patients treated with radical surgery. The mean age of the patients was 62.

Expression of Ki-67 (MIB-1) and GLUT-1 proteins in non-advanced prostatic cancer.

The expression of Ki-67 (MIB-1) and glucose transporter-1 (GLUT-1) were evaluated in patients with clinically localized prostate cancer (PC) who had undergone radical prostatectomy with curative intent. 140 low advanced PC specimens were studied. Protein expression was assessed immunohistochemically on tumour sections and expressed as a labelling index, i.

Impairment of prostate cancer cell growth by a selective and reversible lysine-specific demethylase 1 inhibitor.

Post-translational modifications of histones by chromatin modifying enzymes regulate chromatin structure and gene expression. As deregulation of histone modifications contributes to cancer progression, inhibition of chromatin modifying enzymes such as histone demethylases is an attractive therapeutic strategy to impair cancer growth. Lysine-specific demethylase 1 (LSD1) removes mono- and dimethyl marks from lysine 4 or 9 of histone H3.

Irradiation-induced bone sarcoma in a patient treated for cervix cancer 28 years earlier.

Aim Of The Study: To present a case of a patient with cervical carcinoma in stage IIA who was diagnosed with pelvic bone sarcoma 28 years after radiotherapy.

Case Presentation: A 37-year-old woman with IIA cervix cancer was treated with external beam irradiation and brachytherapy. The patient had undergone conventionally fractionated external beam irradiation using the "box" technique, with the total dose of 50 Gy and brachytherapy with radium applicators (intrauterine tube and fornix applicator) with the dose of 60 Gy calculated at point A.

The intraoperative assessment of the effectiveness of computed tomography in the detection of the residual mass of the retroperitoneal space in patients with nonseminoma testicular tumors after chemotherapy.

Background: The incidence of testicular tumors in Poland accounts for 2.4 new cases per 100.000 men a year.

Principles, implementation, and application of biology-oriented synthesis (BIOS).

Biology-oriented synthesis (BIOS) represents an alternative approach for the generation of compound collections for biological applications. In BIOS, biologically relevant and prevalidated scaffold structures, such as core structures of natural products or known drugs, are employed as scaffolds for the generation of compound collections with focused diversity. In this review, we discuss the underlying concept of the BIOS approach, and its practical implementation in library design and synthesis.

Biology-oriented combined solid- and solution-phase synthesis of a macroline-like compound collection.

Macrolines constitute a class of natural products that has more than 100 members and displays diverse biological activities. These compounds feature a cycloocta[b]indole scaffold that represents an interesting target structure for biology-oriented synthesis (BIOS). We have presented a solid-phase synthesis of isomerically pure cycloocta[b]indoles by employing the Pictet-Spengler reaction and the Dieckmann cyclization as key steps.

Gamma-pyrone natural products--a privileged compound class provided by nature.

In "Biology Oriented Synthesis" (BIOS), the inherent biological relevance of natural products is employed for the design and synthesis of compound libraries. Towards this end, library generation in BIOS is focused on compound classes from biologically relevant space such as the natural product space or also the drug space and only scaffolds of these areas of proven relevance are employed for synthesis of small focused libraries with limited diversity. We here present a short overview of gamma-pyrone natural products, highlighting their biological properties and their potential applicability in a BIOS of a compound library.

Synthesis and unexpected reactivity of Si-H functionalized dithieno[3,2-b:2',3'-d]phospholes.

[reaction: see text]. Si-H functionalized, blue light-emitting dithieno[3,2-b:2',3'-d]phospholes are accessible by reaction of an appropriate bithiophene precursor with a dichlorophosphane. Subsequent functionalization of the central phosphorus center allows for a fine-tuning of the optoelectronic properties of the material.

Amyloidoma and marginal zone malignant lymphoma (MALT type) in the gastric antrum. A case report.

A case of a lymphoma of the marginal zone (MALT type) in the antrum of the stomach concomitant with an amyloidoma immunohistochemically corresponding to amyloid AL has been reported in a 69-year-old woman.

Primary intrapericardial malignant fibrous histiocytoma. A case report.

A case of giant intrapericardial malignant fibrous histiocytoma has been reported. A brief review of primary cardiac tumours has been presented taking into account differential diagnosis of neoplasms of histologically myxomatous nature.

[Validity of alternatives to animal experimentation in biomedical research].

The "Three Rs" definition of alternatives by Russel and Burch (1959) refers to all methods, protocols, or technologies which replace the use of animals altogether, reduce the number of animals required, or refine existing procedures or technologies so as to minimize the level of stress endured by the animal. The move towards alternative methods in biomedical research must be seen as a gradual and evolutionary process during which each candidate method must be considered in a sequence of three consecutive steps, i.e.

[Suitability of various food mixtures for breeding and rearing of beagle dogs. 3. Methods and results for the Ingelheim team].

Two different modes of nutrition during breeding and rearing of dogs are investigated to determine which of them has the better effect. The use of a complete feed for breeding and maintenance (Altromin H) was compared with the use of a special breeding feed (Zahn Z), respectively, together with a special maintenance feed (Zahn H). A palatability test was made, a 2-year test for the effect on breeding, observation of weight gain and food consumption in the rearing phase on all the dogs of the strain Chbi:BEAGLE.

[Study on different kinds of animal feed for their adequateness for breeding and rearing beagle dogs. 1st communication: summarizing presentation of results (author's transl)].

Some of the current food mixtures for dogs available on the market and their further development were tested regarding the usage under rational and standardized experimental conditions. Further, it should be clarified which of the two separate modes of nutrition has the better effect on breeding and rearing of dogs. The use of a complete feed for breeding and maintaining (Altromin H) was compared with a special breeding feed (Zahn Z) together with a special maintenance feed (Zahn H).