[Experimental research of targeting hTERT gene inhibited in hepatocellular carcinoma therapy by RNA interference].

Background & Objective: RNA interference (RNAi) is a new gene blocking technology that silences target gene at post-transcription level induced by the small interference RNA (siRNA). RNAi has been demonstrated great prospect in gene functional research and gene therapy areas. Nowadays, RNAi has been reported to be used to inhibit the expression of endogenous genes including cyclophilin, GAPDH, p53, and c-myc; and there were some progresses in the therapy of the diseases caused by AIDS and hepatitis viruses with RNAi.

The novel organization and complete sequence of the ribosomal RNA gene of Nosema bombycis.

We present here for the first time the complete DNA sequence data (4301bp) of the ribosomal RNA (rRNA) gene of the microsporidian type species, Nosema bombycis. Sequences for the large subunit gene (LSUrRNA: 2497bp, GenBank Accession No. ), the internal transcribed spacer (ITS: 179bp, GenBank Accession No.

[Effects of exercise training during 21 d -6 degrees head down bed rest on dynamic posture equilibrium and motor coordination].

Objective: To study the effects of 21 d -6 degrees head down bed rest (HDBR) with exercise training on dynamic posture equilibrium and motor coordination.

Method: Ten healthy young men were randomly divided into HDBR control groups and HDBR exercise groups, with 5 in each group. The subjects in the exercise group performed exercise with a bicycle ergometer in the supine position 1 h/d during bed rest.

[Study on relationship between emotional stability in flight and nerve system excitability].

Objective: To study the related factors of emotional stability in flight.

Method: Based on the operable definition of emotional stability in flight and the related literature review, 63 experienced pilots and flight coaches were investigated and the other-rating questionnaire of emotional stability in flight was established. To test the senior nerve system, Uchida Kraeplin (UK) test was administrated on 153 19-21 years old male student pilots of the second grade in the department of flight technique in China Civil Aviation College, who were selected through 13 h flight, 35 h solo flight, and acted as the standardization group.

[Detection of platelet Ca2+(i), CD62P, CD63 and plasma CD62P in cirrhosis patients].

Objectives: To study on the relationship between platelet Ca2+(i), CD62P, CD63, serum CD62P (SCD62P) and cirrhosis patients.

Methods: Platelet CD62P, CD63 were determined with flow cytometry, SCD63P with ELISA, and Ca2+(i) in platelet was determined with fluorophotometry.

Results: Platelet Ca2+(i), CD62P, CD63, and SCD62P levels in cirrhosis patients were (103.

[Preventive effects of exercise training on bone loss during 21 d -6 degrees head down bed-rest].

Objective: To observe the protective effects of exercise training during 21 d -6 degrees head down bed rest (HDBR) on bone loss.

Method: Ten healthy young men were randomly divided into HDBR group and HD-BR exercise group, with 5 in each group. Both groups were exposed to -6 degrees head down bed rest for 21 d.

[Effects of +Gx load on energy metabolism of brain tissue in rats].

Objective. To observe the changes of energy metabolism of brain tissue in rats under +Gx loads, and to explore its possible role in changes of brain function and work efficiency induced by +Gx stress. Method.

[Application of lower body negative pressure (LBNP) in aerospace medicine].

Effects of LBNP is similar to that produced by gravitational force, especially as a stress factor on the cardiovascular system as has been concerned in the area of aerospace medicine. This paper described experimental equipment, methods and physiological effects of LBNP, especially its application in the area of aerospace medicine. Several aspects for future research were put forward.

Washing and cryoprotectant effects on frozen storage of spent hen surimi.

Mince of 98-wk-old spent hens was washed two times with 0.1% NaCl. Portions of unwashed and washed mince were mixed with a cryoprotectant (CP) composed of 4% sucrose, 4% sorbitol, and 0.

Differences in molar absorptivity of 4-NP with the reaction solution and apparatus affect ALP measurement.

We examined the differences in molar absorptivity of 4-NP obtained using different kits for ALP measurement and different instruments. The apparent molar absorptivity of 4-NP in the same reaction solution determined by six different instruments was 15.98, 16.

Adulteration by synthetic therapeutic substances of traditional Chinese medicines in Taiwan.

The adulteration by synthetic therapeutic substances of traditional Chinese medicines has been reported on various occasions and has been a public health concern in Taiwan over the past several years. A large-scale effort was initiated in 1992 to screen traditional Chinese medicines that were suspected of adulteration with synthetic therapeutic substances. The term "adulteration" refers to traditional Chinese medicines that are tested and found to contain chemical substances not prescribed or labeled as part of the intended use.

Case-control study of the effectiveness of different types of helmets for the prevention of head injuries among motorcycle riders in Taipei, Taiwan.

A total of 1,351 victims of motorcycle accidents, brought to one of 15 hospitals responsible for emergency care in Taipei, Taiwan, between August 1 and October 15, 1990, were enrolled in a case-control study to investigate the effectiveness of different types of helmets for the prevention of head injuries. A total of 562 of those with head injuries were assigned to the case group, while the remaining 789 victims without head injuries were considered as emergency room controls. The case group was subdivided into daytime and evening cases, according to the time of accident.

[Chemotherapy of biliary tract infections (XXIV). Biliary excretion and gallbladder tissue levels of mezlocillin].

Mezlocillin (MZPC), a new broad spectrum penicillin, was studied for its biliary excretion and levels in gallbladder tissues. The mean serum concentration immediately after intravenous administration of 2 g of MZPC in 6 cholecystectomized patients was found to be 535.5 +/- 43.