Presence of a t(12;18)(q14;q21) Chromosome Translocation and Fusion of the Genes for High-mobility Group AT-Hook 2 () and WNT Inhibitory Factor 1 () in Infrapatellar Fat Pad Cells from a Patient With Hoffa's Disease.

Background/aim: Hoffa's disease is anterior knee pain presumably stemming from inflammatory fibrous hyperplasia of the infrapatellar fat pad (Hoffa's pad). The etiology and pathogenesis are unclear, however, and no genetic information about the disease has been published. We report the genetic findings in cells from the fat pad of a patient with Hoffa's disease.

Quantification of leg oedema in postmenopausal hypertensive patients treated with lercanidipine or amlodipine.

Objective: Of the study was to compare the leg oedema-forming potential of two different dihydropyridine calcium channel blockers in postmenopausal women.

Design: A total of 92 postmenopausal hypertensive patients [systolic blood pressure (SBP) 150-179 mmHg or diastolic blood pressure (DBP) 95-109 mmHg were randomized to receive a 4-week treatment with either 10 mg/day lercanidipine (n = 48) or 5 mg/day amlodipine (n = 44), with force-titration to 20 and 10 mg/day, respectively for an additional 4 weeks.

Methods: Leg volume was measured by water displacement volumetry, patients were questioned for symptoms and a physical examination was performed to detect the presence of oedema.

Relation between low dose of hydrochlorothiazide, antihypertensive effect and adverse effects.

Thiazide diuretics are widely used in the drug treatment of hypertension but their dose-response curves for the antihypertensive and adverse metabolic effects differ. To characterize the lower end of the dose-response curve a double-blind, parallel group trial was performed as multicentre study in Scandinavia. One hundred and eleven patients with newly diagnosed or previously treated mild to moderate hypertension (untreated diastolic blood pressure of 95-115 mmHg after 4 weeks placebo) were randomly allocated to various doses of hydrochlorothiazide (3, 6, 12.

The influence of sustained release theophylline therapy on free fatty acids in serum.

The serum concentrations of theophylline, total free fatty acids (FFA) and subgroups were studied in ten healthy volunteers treated with sustained release theophylline twice daily during 9 days. Analyses were performed before and on day 1, 4 and 9 of treatment. Mean theophylline serum levels within the therapeutic range were obtained during the study, ranging from 62 +/- 7 to 82 +/- 10 mumol/l.

Single dose pharmacokinetics of tiaprofenic acid. Effects of food and severe renal insufficiency.

The single oral dose pharmacokinetics of tiaprofenic acid (Surgam) has been investigated in fasting and non-fasting healthy volunteers (200 and 300 mg) and in fasting patients with severe renal insufficiency (200 mg). A dose independent pharmacokinetics of tiaprofenic acid was shown in fasting healthy volunteers and the following parameters were calculated after administration of 200 mg: tl = 0.53 +/- 0.

Long-term changes in transperitoneal water transport during continuous ambulatory peritoneal dialysis.

9 patients were observed prospectively during 14-40 months 003 continuous ambulatory peritoneal dialysis (CAPD) treatment. From start of CAPD, each patient recorded dwell time, drained ultrafiltration volume (delta V), initial glucose concentration in dialysate, dialy fluid intake, body weight and blood pressure on a special form. These data, together with monthly values for albumin, urea, creatinin, phosphate, glucose and beta 2-microglobulin in plasma and in instilled dialysate, were later fed into a specially designed computer program to compare changes in the monthly mean (+/- SEM) values.

The effect of systemic heparinization on plasma lipoproteins and toxicity in patients on hemodialysis and continuous ambulatory peritoneal dialysis.

The lipid patterns of plasma from 6 patients on hemodialysis (HD) and 6 patients on continuous ambulatory peritoneal dialysis (CAPD) were compared and correlated to plasma toxicity as measured by the survival of human macrophages cultured in vitro. The median values for plasma triglycerides (TG), cholesterol, low density lipoprotein (LDL) cholesterol, apolipoprotein B and lipolytic activities (lipoprotein lipase and hepatic lipase) were insignificantly higher in CAPD plasma than in HD plasma. The median high density lipoprotein (HDL) cholesterol/LDL cholesterol ratio was significantly higher in HD plasma than in CAPD plasma.

Hemodialysis and cell toxicity in vitro related to plasma triglycerides, post-heparin lipolytic activity and free fatty acids.

Plasma triglyceride (TG) concentrations, post-heparin lipolytic activities and the free fatty acid (FFA) pattern of uremic and normal plasma were compared and correlated to plasma toxicity as measured by the effect on human mononuclear phagocytes cultured in vitro. Plasma TG concentration and FFA: albumin molar ratios were significantly higher in uremic plasma, and a correlation was found between TG concentrations prior to heparinization and post-heparin FFA concentrations. Uremic plasma toxicity was significantly correlated to increased post-heparin FFA: albumin molar ratio.

Toxicity of plasma from hemodialysis patients treated with heparin or prostacyclin.

Hemodialysis was performed in 6 uremic patients with either a bolus dose of 5 000 IU heparin or prostacyclin at a constant infusion rate of 5 ng/kg/min. Clinical data, plasma triglycerides (TG), free fatty acids (FFA), platelet aggregation, white blood cell count and plasma toxicity were measured prior to and during both procedures. No serious side-effects were recorded.

Glucose, insulin and C-peptide kinetics during continuous ambulatory peritoneal dialysis.

The insulin and C-peptide kinetics due to glucose (50 g), given intraperitoneally or enterally has been compared in five non-diabetic patients on continuous ambulatory peritoneal dialysis (CAPD). The fasting C-peptide concentrations were three to ten times the normal values whereas the fasting plasma insulin concentrations were within normal limits. After intraperitoneal glucose administration a more marked hyperglycaemia (p less than 0.

Systemic heparinization of uremic patients and its effects on blood lipids and on in vitro toxicity of the plasma.

The effect of heparin on plasma lipids was investigated in uremic patients prior to and during hemodialysis, and in healthy individuals receiving equal heparin doses. The effects of heparin on plasma lipids were correlated with the effects of plasma on phytohemagglutinin (PHA)-stimulated lymphocytes, and on the function of human mononuclear phagocytes cultured in vitro. The plasma concentrations of high density lipoproteins, cholesterol and albumin were not influenced by systemic heparinization or hemodialysis.

Labetalol in the treatment of hypertension in patients with normal and impaired renal function.

Labetalol (Trandate) is a new antihypertensive agent with both alpha- and beta-adrenoceptor blocking properties. In a double-blind cross-over study the antihypertensive action and side-effects of labetalol and propranolol were compared in 18 previously untreated outpatients with hypertension, WHO stage I--III. Mean daily dose of labetalol was 667 mg and of propranolol 129 mg.

The effect of serum and plasma from haemodialysis patients on human mononuclear phagocytes cultured in vitro.

Human mononuclear phagocytes from healthy individuals were cultured in plasma and sera from normal persons and patients with chronic renal failure receiving haemodialysis. There was a marked detachment of macrophages from the glass coverslips when the cells were grown for 4 days in uraemic serum or plasma compared to normal serum. The ability of the macrophages to adhere to the glass coverslips was reduced when the cells were cultured in plasma from normal persons and uraemics subjected to systemic heparinization.

The effect of uraemic serum and plasma on proliferating cells in vitro.

Phytohaemagglutinin (PHA)-stimulated lymphocytes from healthy individuals and a malignant cell-line (K-562 cells) were cultured in a growth medium of 75 per cent RPMI 1640 (Gibco) supplemented with 25 per cent of uraemic serum or plasma obtained after systemic heparinization. Pooled human A Rh+ serum from 3 different donors served as controls. There was a depression of the DNA synthesis in both cell systems measured as uptake of methyl-3H-thymidine when the cells were cultured in uraemic milieu.

Serum levels of mecillinam in patients with severely impaired renal function.

12 patients with severe renal insufficiency were treated for urinary tract infection with 400 mg of mecillinam intravenously every 6 h. High serum concentrations of mecillinam were found 6 h after the first morning dose on day 2 and day 5 of the treatment period (mean values 11.4 and 14.