Prognostic Value of Surgical Resection for Non-small-cell Lung Cancer Patients Comorbid With Minimal Pleural Effusion.

Background: The proportion and impact of minimal pleural effusion (PE) on prognosis remain blurred in operable non-small cell lung cancer (NSCLC) patients who reported minimal PE on imaging.

Methods: Clinical and prognostic data of operable NSCLC patients who presented no distant metastasis, no direct pleural invasion, but minimal PE on preoperative imaging were retrospectively analyzed. The patients were divided into surgical (81 cases) and non-surgical (10 cases) cohorts.

Rice E3 ubiquitin ligases balance immunity and yield through non-proteolytic ubiquitination.

The rice E3 ubiquitin ligases OsCIE1 and IPI7 mediate the non-proteolytic polyubiquitination of the pattern-recognition receptor kinase OsCERK1 and the transcription factor IPA1, respectively, in response to Magnaporthe oryzae infection, thereby fine-tuning rice growth-immunity trade-offs.

Innovations in Transgene Integration Analysis: A Comprehensive Review of Enrichment and Sequencing Strategies in Biotechnology.

Understanding the integration of transgene DNA (T-DNA) in transgenic crops, animals, and clinical applications is paramount for ensuring the stability and expression of inserted genes, which directly influence desired traits and therapeutic outcomes. Analyzing T-DNA integration patterns is essential for identifying potential unintended effects and evaluating the safety and environmental implications of genetically modified organisms (GMOs). This knowledge is crucial for regulatory compliance and fostering public trust in biotechnology by demonstrating transparency in genetic modifications.

DMSO-promoted α-bromination of α-aryl ketones for the construction of 2-aryl-2-bromo-cycloketones.

A DMSO-promoted practical one-step α-bromination reaction of α-aryl ketones with NBS has been developed for the construction of 2-aryl-2-bromo-cycloketones. The desired regioselective α-bromination products were isolated in moderate to good yields, with a maximum tested scale of 15 mmol. Notably, ketamine derivatives could be smoothly synthesized in two steps.

Donor-Acceptor Functionalized Water-Soluble Metal-Organic Cages Showing an Excellent Synergistic Photothermal-Chemotherapy Effect.

Water-soluble metal-organic cages (WSMOCs) show high potential as antitumor agents, while the targeted functionalization of WSMOCs toward enhanced antitumor performances is a challenging task. Herein, WSMOCs were functionalized with donor-acceptor (D-A) systems for synergistic photothermal-chemotherapy. Octahedral [ML] cages based on a 2,4,6-tri(2-pyridine-4-yl)-1,3,5-triazine (TPT) acceptor and M(bpy) (M = Pd for , Pt for ) nodes were functionalized with tetrathiafulvalene (TTF) to form and .

In Situ Cascade Catalytic Polymerization of Dopamine Based on Pt NPs/CoSAs@NC Nanoenzyme for Constructing Highly Sensitive Photocurrent-Polarity-Switching PEC Biosensing Platform.

Nanozymes open up new avenues for amplifying signals in photoelectrochemical (PEC) biosensing, which are yet limited by the generated small-molecule signal reporters. Herein, a multifunctional nanoenzyme of Pt NPs/CoSAs@NC consisting of Co single atoms on N-doped porous carbon decorated with Pt nanoparticles is successfully synthesized for cascade catalytic polymerization of dopamine for constructing a highly sensitive photocurrent-polarity-switching PEC biosensing platform. Taking protein tyrosine phosphatase 1B (PTP1B) as a target model, Pt NPs/CoSAs@NC nanoenzymes are linked to magnetic microspheres via phosphorylated peptides.

Tumor Mutation Signature Reveals the Risk Factors of Lung Adenocarcinoma with or Mutation.

Introduction: and mutations are frequently detected in lung adenocarcinoma (LUAD). Tumor mutational signature (TMS) determination is an approach to identify somatic mutational patterns associated with pathogenic factors. In this study, through the analysis of TMS, the underlying pathogenic factors of LUAD with and mutations were traced.

A SARS-CoV-2 EG.5 mRNA vaccine induces a broad-spectrum immune response in mice.

The emerging of emergent SARS-CoV-2 subvariants has reduced the protective efficacy of COVID-19 vaccines. Therefore, novel COVID-19 vaccines targeting these emergent variants are needed. We designed and prepared CoV072, an mRNA-based vaccine against SARS-CoV-2 Omicron (EG.

Characterization of the hepatic flora and metabolome in nonalcoholic fatty liver disease.

Background/aim: The purpose of this study was to examine the hepatic bacterial composition and metabolome characteristics of patients with NAFLD using 16S rDNA sequencing and metabolomics. The results of the study revealed substantial differences in hepatic bacterial composition and metabolites between the NAFLD group and the control group. These differences were used to identify potential biomarkers that could be employed to diagnose NAFLD.

The protective role of baicalin regulation of autophagy in cancers.

Autophagy is a conservative process of self degradation, in which abnormal organelles, proteins and other macromolecules are encapsulated and transferred to lysosomes for subsequent degradation. It maintains the intracellular balance, and responds to cellular conditions such as hunger or stress. To date, there are mainly three types of autophagy: macroautophagy, microautophagy and chaperone-mediated autophagy.

Construction and high-throughput screening of gradient nanowire coatings on titanium surface towards ameliorated osseointegration.

Surface nano-modification has emerged as an effective strategy to enhance osseointegration of titanium (Ti) implants. Despite its promise, rational optimization of surface nanomorphology for ameliorated osseointegration remains a significant challenge. Our research pioneering developed a one-step alkali etching technique to produce a gradient nanowire coating with continuously varied dimensions on Ti surfaces, which was subsequently served as a versatile platform for high-throughput screening of optimal dimensions to enhance osseointegration.

TClC effectively suppresses the growth and metastasis of NSCLC via polypharmacology.

Despite significant advances in targeted therapies and immunotherapies, non-small cell lung cancer (NSCLC) continues to present a global health challenge, with a modest five-year survival rate of 28 %, largely due to the emergence of treatment-resistant and metastatic tumors. In response, we synthesized a novel bioactive compound, ethyl 6-chlorocoumarin-3-carboxylyl L-theanine (TClC), which significantly inhibited NSCLC growth, epithelial mesenchymal transition (EMT), migration, and invasion and tumor growth and metastasis without inducing toxicity. TClC disrupts autocrine loops that promote tumor progression, particularly in stem-like CD133-positive NSCLC (CD133+ LC) cells, which are pivotal in tumor metastasis.

Novel carbon dots with dual Modulatory effects on the bone marrow and spleen as a potential therapeutic candidate for treating spinal cord injury.

Spinal cord injury triggers leukocyte mobilization from the peripheral circulation to the injury site, exacerbating spinal cord damage. Simultaneously, bone marrow hematopoietic stem cells (HSCs) and splenic leukocytes rapidly mobilize to replenish the depleted peripheral blood leukocyte pool. However, current treatments for spinal cord injuries overlook interventions targeting peripheral immune organs and tissues, highlighting the need to develop novel drugs capable of effectively regulating peripheral immunity and treating spinal cord injuries.

Resveratrol promotes cholesterol efflux from dendritic cells and controls costimulation and T-cell activation in high-fat and lipopolysaccharide-driven atherosclerotic mice.

Cholesterol aggregation in dendritic cells (DCs) triggers an inflammatory response and accelerates the development of atherosclerosis (AS). Resveratrol (RES), a natural compound with anti-inflammatory and cholesterol metabolism regulatory properties, has been shown to influence the maturation and inflammatory functions of DCs. However, its relationship with cholesterol metabolism remains unclear.

The study on cuproptosis in Alzheimer's disease based on the cuproptosis key gene .

Background: Alzheimer's disease (AD) is a neurodegenerative disorder characterized by memory and cognitive impairments. Previous studies have shown neuronal death in the brains of AD patients, but the role of cuproptosis and its associated genes in AD neurons remains unclear.

Methods: Intersection analysis was conducted using the AD transcriptome dataset GSE63060, neuron dataset GSE147528, and reported cuproptosis-related genes to identify the cuproptosis key gene highly expressed in AD.

Development and validation of a nomogram for predicting sleep disturbance in pregnant and postpartum women: A pilot study.

Background: Sleep disturbances are common in pregnant and postpartum women, impacting their health. Predictive tools for timely intervention are scarce.

Objective: To develop and validate a nomogram predicting sleep disturbance risk in this demographic.

Guilu Erxian glue reduces endoplasmic reticulum stress-mediated apoptosis and restores the balance of extracellular matrix synthesis and degradation in chondrocytes by inhibiting the ATF6/GRP78/CHOP signaling pathway.

Knee Osteoarthritis (KOA) is characterized by phenotypic alterations, apoptosis, and the breakdown of the extracellular matrix (ECM) in the superficial articular cartilage cells. The inflammatory response activates the Endoplasmic Reticulum Stress (ERS) signaling pathway, which plays a critical role in the pathophysiology and progression of KOA. Chondrocytes stimulated by thapsigargin(TG)exhibit heightened ERS and significantly increase the expression of ERS-associated proteins.

Baicalein inhibits NLRP3 inflammasome activation and mitigates placental inflammation and oxidative stress in gestational diabetes mellitus.

Gestational diabetes mellitus (GDM) is a common metabolic disorder during pregnancy characterized by glucose intolerance, which poses risks to both maternal and fetal health. Baicalein, a flavonoid derived from the roots of Georgi, exhibits various biological functions and has been implicated in the modulation of several diseases. However, the regulatory effects and underlying mechanisms of Baicalein in GDM progression remain unclear.

Reprogramming of fibroblasts into cancer-associated fibroblasts via IGF2-mediated autophagy promotes metastasis of lung cancer cells.

Cancer-associated fibroblasts (CAFs) are major component of stromal cells. Growing evidence suggests that CAFs promote tumor growth and metastasis; however, the reprogramming of normal fibroblasts (NFs) into CAFs by tumor cells still remains largely unknown. In this study, we found that non-small cell lung cancer (NSCLC) cells activated NFs into CAFs via autophagy induction.

C5aR1-positive adipocytes mediate non-shivering thermogenesis in neonatal mice.

Brown adipose tissue (BAT) plays an important role in maintaining body temperature in newborn mammals; however, its mechanisms remain poorly understood. Here, we report the identification of a special population of brown adipose tissue-derived stromal cells (ASCs) in neonatal mice that highly express CD45 and can be differentiated into adipocytes with lower thermogenic ability. These CD45 adipocytes also characteristically contained complement C5a receptor 1(C5aR1) on the cell membrane.