Circulating immune complexes during immunotherapy in allergy to dog.

Circulating immune complexes (CIC) were determined from dog-allergic asthmatic children (n = 35) receiving immunotherapy with dog dander and hair extract. The results from CIC are expressed in SDU (standard deviation units) and presented as follows: pretreatment results (n = 20), rush results (n = 11), mid-schedule results (n = 20), maintenance results (n = 15) and the results of the placebo-treated group (n = 12). The results of the placebo-treated group (n = 12) and those of the untreated atopic (n = 12) and non-atopic (n = 14) were controls.

[Malignant neuroleptic syndrome. Literature review and case report].

The neuroleptic malignant syndrome, first described by Delay and Deniker in 1968, is a rare but severe complication of neuroleptic treatment. According to the literature the syndrome particularly affects young men undergoing rapidly intensified neuroleptic treatment ("rapid neuroleptization"). The diagnosis is based on the triad of hyperthermia, extrapyramidal signs, and autonomic dysfunction, once infectious disease has been ruled out.

Serum immune complexes and rheumatoid factors in superficial bladder tumours.

Serum immune complex (IC) and rheumatoid factor (RF) levels of 39 patients with a transitional cell tumour of the bladder were analyzed using 4 different IC and 2 RF assays. Elevated serum immune complex and rheumatoid factor levels were observed in the majority of the patients (95%). Conglutinin-binding (KgB), C1q-binding (C1qB), platelet iodinated protein A test (PIPA) and RF-enzyme immunoassay (RF-EIA) gave positive results in 31%, 62%, 23% and 31% of the cases, respectively and separated cancer sera from normal blood donors (p less than 0.

Elevated serum immune complex levels in Pogosta disease, an acute alphavirus infection with rash and arthritis.

Circulating immune complexes (CIC) were studied in Pogosta disease, an acute alphavirus infection with fever, rash and arthritis. The disease is caused by a virus antigenically closely related to Sindbis virus. 75 serum specimens from 25 patients with serologically verified infection were obtained from 1-87 days after the onset.

Natural course of juvenile autoimmune thyroiditis.

Forty-six patients with juvenile autoimmune thyroiditis were followed for an average of 6.5 years. The diagnosis was based on a firm goiter and on cytologic findings of lymphocytic thyroiditis.

Circulating immune complexes in hypertensive disorders of pregnancy.

Circulating immune complexes (CICs) were measured in sera of pregnant women with pre-eclampsia and other hypertensive disorders of pregnancy and pregnant women with renal disease, using four different CIC assays: platelet 125I-labelled staphylococcal protein A test ( PIPA ), conglutinin-binding ELISA, C1q-binding ELISA and rheumatoid factor binding inhibition ELISA. CICs were shown to be present in the sera of 47% of women with severe pre-eclampsia, in 20% with mild pre-eclampsia and in 18% of women with normal pregnancy using the PIPA test. The PIPA test was capable of discriminating between patients with renal disease, which were all positive, and women with uncomplicated hypertension, which were all negative.

Clinical correlates of circulating immune complexes in patients with recent yersiniosis.

Circulating immune complexes (CICs) and rheumatoid factor were studied in 31 patients with serologically confirmed yersiniosis (12 in a pilot series and 19 in a prospective series). Yersiniosis is an intestinal infection complicated occasionally by extraintestinal symptoms such as aseptic arthritis. Four tests representing three main principles (affinity of human platelets and of C1q for complexed IgG and of conglutinin for C3) were used for the detection of CICs, which were found in all patients.

Circulating immune complexes and rheumatoid factors in patients with farmer's lung.

Circulating IgG immune complexes (CICs), IgM rheumatoid factors (RFs), complement level (C3 and C4), and IgG antibodies to Aspergillus fumigatus, Aspergillus umbrosus, Thermoactinomyces vulgaris, and Micropolyspora faeni were analysed in the sera of 14 patients with farmer's lung (FL), 10 in the acute and four in the subacute phase of the disease. Ten spouses of FL patients served as exposed healthy controls. C3 and C4 were measured fluoronephelometrically.

Immune complexes in serum and cerebrospinal fluid of multiple sclerosis patients and patients with other neurological diseases.

Paired serum and cerebrospinal fluid (CSF) specimens from 30 multiple sclerosis (MS) patients and 30 patients with other neurological diseases (ONDs) were analyzed for the presence of immune complexes (ICs). With each of the 4 tests used, ICs were found more frequently in sera from both MS and OND patients than in sera from healthy blood donors. IC-positivity for MS and OND patient CSF varied from 10-33% and from 10-17% in different tests.

Interference by rheumatoid factor and C1q in the detection of IgG complexes: studies of model systems by ELISA.

Interference by purified IgM rheumatoid factors (RFs) and C1q in the detection of model complexes was studied by enzyme-linked immunosorbent assay (ELISA). Soluble monoclonal and polyclonal cryoglobulin IgM RFs and human or porcine C1q inhibited dose-dependently the binding of human IgG complexes to solid-phase IgM RF, C1q, and bovine conglutinin (Kg). The inhibition patterns of the Kg binding were dependent on the order of confrontation between the reactants.

Evaluation of six tests for circulating IgG complexes with special reference to IgM rheumatoid factors: analysis of systemic lupus erythematosus and rheumatoid arthritis series.

Six tests for circulating immune complexes (CIC) developed in four laboratories and representing four main principles [affinity of human platelets, Clq, of RF for aggregated IgG, and of conglutinin (Kg) for complex-bound C3] were evaluated on series of SLE and definite RA. All tests detected human model complexes in the presence of NHS and discriminated the patient series from the blood donor series, most powerful being the PIPA (platelet test). The high correlation between the RF-binding inhibition tests (RFbI) and the RF-latex test suggested interference due to intrinsic RFs.

Significance of monospecific antisera in the diagnosis and prognosis of autoimmune haemolytic anaemias.

The significance of the monospecific antiglobulin test in the diagnosis and prognosis of autoimmune haemolytic anaemia (AIHA) was tested in 74 patients with AIHA and 59 patients with secondary AIHA. There were 48 female and 26 male patients. Idiopathic AIHA patients more often had two or more positive monospecific reactions in the direct antiglobulin test than secondary AIHA patients.

Circulating immune complexes, the concentration of serum IgA and the distribution of HLA antigens in IgA nephropathy.

A series of 40 IgA nephropathy patients is presented. IgA nephropathy was the most common (24%) type of glomerulonephritis in our renal biopsy material. Circulating immune complexes were measured by five methods that gave a positive result in 9-69% of the patients.

Circulating immune complexes, immunoconglutinins, and rheumatoid factors in nephropathia epidemica.

Circulating immune complexes (CICs), immunoconglutinins, and antiglobulins were studied in nephropathia epidemica, an acute infectious hemorrhagic fever occurring in Finland and Scandinavia. Sixty-one serum specimens from 18 serologically confirmed patients were collected between day -5 and day 230 from the onset of fever. Five CIC tests, three immunoconglutinin tests, and various other tests were used to characterize the disease immunologically.

Rheumatoid factor in acute viral infections: interference with determination of IgM, IgG, and IgA antibodies in an enzyme immunoassay.

IgM rheumatoid factor (RF), an autoantibody to the Fc fragment of IgG, was determined by solid-phase enzyme immunoassay (EIA). RF levels were significantly higher in patients with rubella virus infection than in patients with infections due to influenza virus, cytomegalovirus, respiratory syncytial virus, parainfluenza virus, adenovirus, mumps virus, or herpes simplex virus. To evaluate the role of RF in EIA determinations of viral antibodies, IgM RF from IgM-IgG cryoglobulin or control IgM was added to patient sera before assay for viral antibodies.

Glomerular electron-dense deposits and circulating immune complexes in patients with malignant tumours.

39 patients with malignant neoplasms were studied for the presence of paraneoplastic glomerular disease. The nephrotic syndrome was found in none of the patients. From 24 patients an adequate renal biopsy specimen was available for examination by electron microscopy.

Effects of immune complexes on plasma triglycerides in rabbits.

The effect of immune complexes on plasma lipids, especially triglycerides (TG) was studied in rabbits. After a single intravenous dose of bovine serum albumin (BSA), serological aberrations suggestive of immune complexes appeared around the 14th day. Changes in the plasma TG or cholesterol values were not observed within a follow-up period of 4 weeks.