Publications by authors named "W-L Lao"
Proc Natl Acad Sci U S A
February 2020
Article Synopsis
- Transcriptome profiling using RNA-seq typically requires the synthesis of second-strand complementary DNA (cDNA), which can complicate library preparation.
- The new method, SHERRY, uses bacterial transposase Tn5 to construct RNA-seq libraries by directly adding sequencing adapters to RNA after reverse transcription, eliminating the need for second-strand synthesis.
- SHERRY is adaptable for various starting materials, from bulk RNA to single cells, and offers a simpler protocol that improves reproducibility and GC uniformity compared to traditional RNA-seq methods.
