Morphological effects of porous poly-d,l-lactic acid/hydroxyapatite scaffolds produced by supercritical CO2 foaming on their mechanical performance.

A novel supercritical CO2 foaming technique was used to fabricate scaffolds of controllable morphology and mechanical properties, with the potential to tailor the scaffolds to specific tissue engineering applications. Biodegradable scaffolds are widely used as temporary supportive structures for bone regeneration. The scaffolds must provide a sufficient mechanical support while allowing cell attachment and growth as well as metabolic activities.

Three-dimensional porous bioscaffolds for bone tissue regeneration: fabrication via adaptive foam reticulation and freeze casting techniques, characterization, and cell study.

Highly interconnected and 3D porous bioactive hydroxyapatite (HAP) and Bioglass scaffolds have been fabricated by an adaptive version of camphene based foam reticulation (ARM) and camphene freeze casting (CFC) methods. Controlled sublimation of camphene during freeze casting at -78°C produced process optimized bioscaffolds with open, uniform, and interconnected porous structures. HAP and Bioglass scaffolds with desired porosity, pore size, and microtopography were successfully fabricated using polyurethane foam templates of appropriate structures.

Synergizing metabolic flux analysis and nucleotide sugar metabolism to understand the control of glycosylation of recombinant protein in CHO cells.

Background: The glycosylation of recombinant proteins can be altered by a range of parameters including cellular metabolism, metabolic flux and the efficiency of the glycosylation process. We present an experimental set-up that allows determination of these key processes associated with the control of N-linked glycosylation of recombinant proteins.

Results: Chinese hamster ovary cells (CHO) were cultivated in shake flasks at 0 mM glutamine and displayed a reduced growth rate, glucose metabolism and a slower decrease in pH, when compared to other glutamine-supplemented cultures.

Ontogenesis of laterality in 3- to 10-yr.-old children: increased unimanual independence grounded on improved bimanual motor function.

Underlying sensorimotor factors, such as intermanual coupling, contributing to optimal laterality, long remain immature. Using the handmotor laterality blackboard, developmental change in symmetric bimanual motor function for 413 children in groups of 3 to 10 years of age shows synchronicity increased between groups 3 and 5 years of age; but between groups 5 and 7 years of age, given immature coupling, one hand may still disturb the movements of the other one. Between groups 7 and 9 years of age, the hands gradually stop disturbing each other and move independently and fluently.

Effect of feed and bleed rate on hybridoma cells in an acoustic perfusion bioreactor: metabolic analysis.

For the development of optimal perfusion processes, insight into the effect of feed and bleed rate on cell growth, productivity, and metabolism is essential. In the here presented study the effect of the feed and bleed rate on cell metabolism was investigated using metabolic flux analysis. Under all tested feed and bleed rates the biomass concentration as calculated from the nitrogen balance (biomass-nitrogen) increased linearly with an increase in feed rate, as would be expected.