Publications by authors named "W Z Cande"
, the causative agent of African sleeping sickness, has a flagellum that is crucial for motility, pathogenicity, and viability. In most eukaryotes, the intraflagellar transport (IFT) machinery drives flagellum biogenesis, and anterograde IFT requires kinesin-2 motor proteins. In this study, we investigated the function of the two kinesin-2 proteins, TbKin2a and TbKin2b, in bloodstream form trypanosomes.
View Article and Find Full Text PDFMeiotic double-strand breaks (DSBs) are generated by the evolutionarily conserved SPO11 complex in the context of chromatin loops that are organized along axial elements (AEs) of chromosomes. However, how DSBs are formed with respect to chromosome axes and the SPO11 complex remains unclear in plants. Here, we confirm that DSB and bivalent formation are defective in maize spo11-1 mutants.
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- - Centrioles are essential organelles in eukaryotic cells that play a crucial role in forming cilia and during cell division.
- - In the study of Naegleria amoebae, researchers observed how these organisms rapidly transition from no centrioles to two; the first centriole is formed independently (de novo) and the second is formed next to the first through a process called mentored assembly.
- - The findings suggest that both methods of centriole assembly (de novo and mentored) are ancient and conserved across eukaryotes, fulfilling different but complementary roles in regulating centriole formation.
The binucleate pathogen Giardia intestinalis is a highly divergent eukaryote with a semiopen mitosis, lacking an anaphase-promoting complex/cyclosome (APC/C) and many of the mitotic checkpoint complex (MCC) proteins. However, Giardia has some MCC components (Bub3, Mad2, and Mps1) and proteins from the cohesin system (Smc1 and Smc3). Mad2 localizes to the cytoplasm, but Bub3 and Mps1 are either located on chromosomes or in the cytoplasm, depending on the cell cycle stage.
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- * Researchers used affinity purification and mass spectrometry to discover over 80 potential actin-binding proteins in Giardia, some of which resemble known actin-associated proteins.
- * The study validated the localization and interaction of seven proteins, suggesting that Giardia, despite lacking typical actin-binding proteins, retains a crucial set of actin-interacting proteins that may reflect early evolutionary functions of the actin cytoskeleton.