How Malaysian Adolescents Navigate Sexual Health Information on the Internet.

Significant sociocultural barriers to sexual and reproductive health (SRH) information influence what is acceptable to discuss and learn within the Malaysian community. To address gaps in their knowledge, adolescents routinely turn to the Internet. This study identifies the ways they navigate SRH information found online.

Unravelling Antimicrobial Resistance in : Genetic Mechanisms and Future Directions.

Antimicrobial resistance (AMR) in , the causative agent of Enzootic Pneumonia in swine, poses a significant challenge to the swine industry. This review focuses on the genetic foundations of AMR in , highlighting the complexity of resistance mechanisms, including mutations, horizontal gene transfer, and adaptive evolutionary processes. Techniques such as Whole Genome Sequencing (WGS) and multiple-locus variable number tandem repeats analysis (MLVA) have provided insights into the genetic diversity and resistance mechanisms of .

DNA methylation analysis to differentiate reference, breed, and parent-of-origin effects in the bovine pangenome era.

Background: Most DNA methylation studies have used a single reference genome with little attention paid to the bias introduced due to the reference chosen. Reference genome artifacts and genetic variation, including single nucleotide polymorphisms (SNPs) and structural variants (SVs), can lead to differences in methylation sites (CpGs) between individuals of the same species. We analyzed whole-genome bisulfite sequencing data from the fetal liver of Angus (Bos taurus taurus), Brahman (Bos taurus indicus), and reciprocally crossed samples.

Evaluating the Efficacy of Target Capture Sequencing for Genotyping in Cattle.

(1) Background: Target capture sequencing (TCS) is potentially a cost-effective way to detect single-nucleotide polymorphisms (SNPs) and an alternative to SNP array-based genotyping. (2) Methods: We evaluated the effectiveness and reliability of TCS in cattle breeding scenarios using 48 female and 8 male samples. DNA was extracted from blood samples, targeted for 71,746 SNPs with TWIST probes, and sequenced on an MGI platform.