Publications by authors named "W Woudstra"
Researching disinfection strategies is pivotal because effectively eliminating bacteria and their byproducts during root canal treatment (RCT) remains a challenge. This study investigated the antimicrobial efficacy of natural antimicrobial compounds, propolis (PRO) and copaiba oil-resin (COR), compared to conventional agents in Endodontics. Antimicrobials were tested against endodontic pathogens via macrodilution with standardized inoculums to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC).
View Article and Find Full Text PDFFree radical detection in precision-cut mouse liver slices with diamond-based quantum sensing.
Proc Natl Acad Sci U S A
October 2024
Article Synopsis
- - Free radicals are important in various biological processes like cell communication and aging, and their levels increase in stressed cells, especially in liver diseases and cancer.
- - Detecting free radicals is difficult due to their small size and short lifespan, but diamond-based quantum sensing has been developed to measure them in living cells.
- - The method was adapted for fragile tissues, allowing researchers to successfully track free radical generation in liver slices under stress and observe the effects of antioxidants.
Huntington's disease (HD) is a well-studied yet rare disease caused by a specific mutation that results in the expression of polyglutamine (PolyQ). The formation of aggregates of PolyQ leads to disease and increases the level of free radicals. However, it is unclear where free radicals are generated and how they impact cells.
View Article and Find Full Text PDFAlthough free radicals, which are generated by macrophages play a key role in antimicrobial activities, macrophages sometimes fail to kill () as bacteria have evolved mechanisms to withstand oxidative stress. In the past decades, several ROS-related staphylococcal proteins and enzymes were characterized to explain the microorganism's antioxidative defense system. Yet, time-resolved and site-specific free radical/ROS detection in bacterial infection were full of challenges.
View Article and Find Full Text PDFA dynamic flow model mimicking duodenoscope reprocessing after bacterial contamination for translational research.
Antimicrob Steward Healthc Epidemiol
September 2022
Objective: Duodenoscopy-associated infections and outbreaks are reported globally despite strict adherence to duodenoscope reprocessing protocols. Therefore, new developments in the reprocessing procedure are needed.
Design: We evaluated a novel dynamic flow model for an additional cleaning step between precleaning and manual cleaning in the reprocessing procedure.