Persistence of IgG, IgM, and IgE antibodies in human trichinosis.

An enzyme-linked immunosorbent assay (ELISA) was used to detect serum IgG, IgM, and IgE antibodies against Trichinella spirals s. str. following an outbreak of human trichinosis in 1989 in Chiang Rai Province, Thailand, for two years and seven months in order to observe diagnostic sensitivity of ELISA as well as the persistence of antibodies.

The value of enzyme-linked immunosorbent assay (ELISA) for diagnosis of human trichinosis.

Sera were collected from proven cases of trichinosis and other helminthic infections and examined for IgG and IgM antibodies by an enzyme-linked immunosorbent assay (ELISA). IgG-ELISA was positive in 68.75, 100, and 100% of cases on 23rd, 50th, 57th day of infection while IgM-ELISA was positive in 12.

Serodiagnosis of human gnathostomiasis.

An enzyme-linked immunosorbent assay (ELISA) was evaluated for serodiagnosis of human ocular and visceral gnathostomiasis in comparison to an indirect haemagglutination (IHA) and precipitin (PPT) tests. The ELISA antibody titers were found to range from 1:400 to 1:51,200 against somatic and 1:200 to 1:25,600 against excretory-secretory (ES) antigens. When sera were tested at single dilutions, the ELISA was positive in 7 of 8 gnathostomiasis cases while only 5 and 3 were positive by IHA and PPT respectively.

Prevalence of Toxoplasma antibodies in Chiang Mai population.

Five hundred sera from blood donors were examined for antibodies to Toxoplasma by the indirect hemagglutination antibody (IHA) and the indirect fluorescent antibody (IFA) techniques. In addition, the IHA test was used to detect Toxoplasma antibodies in 500 pregnant women's sera. It was found that 4.