Synthesis and Characterization of Iron Complexes based on Bis-Phosphinite PONOP and Bis-Phosphite PONOP Pincer Ligands.

A series of bis-phosphinite and bis-phosphite PONOP iron complexes were prepared and characterized by NMR and IR spectroscopy. Bis-phosphinite PONOP iron dichloride complexes (PONOP)FeCl (PONOP = 2,6-(RPO)(CHN) and R = Pr, Bu) were prepared through complexation of the free ligands with FeCl and their solid-state structures were determined. Bis-phosphite PONOP iron complexes (PONOP)Fe(PMe) and (PONOP)Fe(PMe) (Cat = catechol) were synthesized through complexation of the free ligands to Fe(PMe).

Fowl cholera immunity induced by various vaccines in broiler minibreeder chickens determined by enzyme-linked immunosorbent assay.

Broiler minibreeder hens were vaccinated for protection against fowl cholera at 12 and 21 weeks of age using several vaccination schemes, which included a live Pasteurella multocida (CU strain) vaccine, two commercial polyvalent fowl cholera oil-based bacterins, and two experimentally prepared polyvalent oil-based bacterins. Some treatment groups received only live or killed vaccines, whereas others received a live vaccine at 12 weeks followed by a killed product at 21 weeks. At 42 weeks of age, all birds that received the live CU vaccine twice or once followed by a bacterin survived challenge.

Comparison of various antigens and their ability to detect protective antibodies against Pasteurella multocida using enzyme-linked immunosorbent assay.

Various antigenic extracts of the CU strain of Pasteurella multocida were prepared to determine their suitability as plate antigens for use in the enzyme-linked immunosorbent assay (ELISA) for the detection of fowl cholera antibodies. Antisera from two separate broiler breeder flocks with known fowl-cholera-vaccination histories were collected just before the birds were challenged with virulent strain X-73 P. multocida.

Response of broiler-type chickens to live Pasteurella multocida--duration of immunity and minimum dose.

Broiler-type chickens were exposed to avirulent Pasteurella multocida by stick-wing once or twice, and groups were challenged with pathogenic P. multocida at 20, 50, or 80 weeks postexposure. Immunity was not lower when chickens were challenged at 80 weeks rather than at 20 and 50 weeks postexposure.

Reaction of bobwhites, coturnix quail, guinea fowl, and mallards to avirulent and virulent Pasteurella multocida.

The CU strain of Pasteurella multocida was moderately virulent for coturnix quail by the oral route and highly virulent for bobwhites by stick-wing. The strain was avirulent for guinea fowl and provided protective immunity by oral or stick-wing routes. Mallards did not react to virulent P.