Label-free visualization of unfolding and crosslinking mediated protein aggregation in nonenzymatically glycated proteins.

Nonenzymatic glycation (NEG) unfolds and crosslinks proteins, resulting in aggregation. Label-free evaluation of such structural changes, without disturbing molecular integrity, would be beneficial for understanding the fundamental mechanisms of protein aggregation. The current study demonstrates the assessment of NEG-induced protein aggregation by combining autofluorescence (AF) spectroscopy and imaging.

Disseminated herpes zoster with varicella encephalitis and pneumonia following ChAdOx1 nCoV-19 (AZD1222) vaccine in an immunocompetent male-a case report.

A middle-aged gentleman, presented to our outpatient department with painful skin lesions suggestive of disseminated herpes zoster. Further examination revealed bilateral cerebellar signs. He had a history of receiving a third dose of AZD1222 vaccine fourteen days prior to the onset of skin lesions but had no other significant medical history.

Structural and biochemical characterisation of the N-carbamoyl-β-alanine amidohydrolase from Rhizobium radiobacter MDC 8606.

N-carbamoyl-β-alanine amidohydrolase (CβAA) constitutes one of the most important groups of industrially relevant enzymes used in the production of optically pure amino acids and derivatives. In this study, a CβAA-encoding gene from Rhizobium radiobacter strain MDC 8606 was cloned and overexpressed in Escherichia coli. The purified recombinant enzyme (RrCβAA) showed a specific activity of 14 U·mg using N-carbamoyl-β-alanine as a substrate with an optimum activity at 55 °C and pH 8.

Impact of a Community-Based Weight Management Program in a North Carolina Health Care System.

Background And Objectives: Current strategies for obesity management in primary care leave many patients inadequately treated or unable to access treatment entirely. We aimed to evaluate a comprehensive, primary care clinic-based weight management program's clinical effectiveness in a community practice setting.  Methods:  This was an 18-month pre/postintervention study.

Anthropometric Indices and Type 2 Diabetes Mellitus as a Risk Factor in Predicting Nonalcoholic Fatty Liver Disease - A Prospective Study in Indian Population.

Background: The different anthropometric indices have different predictive values of nonalcoholic fatty liver disease (NAFLD) in various populations. Since obesity is a common cause of NAFLD and diabetes, therefore, it is critical to correlate the various anthropometric indices as a risk factor in terms of NAFLD and diabetes in the Indian population. In view of reported association between obesity and NAFLD, the study was employed to analyze the relationship of various anthropometric indices (body mass index [BMI], a body shape index [ABSI], waist-height ratio [WHtR], etc.