Publications by authors named "W Sereepapong"
Determining Factors Influencing The Successful Embryo Transfer and Pregnancy during The Frozen Cycle of Fertilization: A Retrospective Cohort Study.
Int J Fertil Steril
October 2024
Background: Frozen embryo transfer (FET) has been increasingly used due to advancements in cryopreservation techniques and the safety advantages. This study aims to determine various factors influencing the successful FET.
Materials And Methods: Retrospective cohort analysis included 1112 women who underwent programmed FET between January 2012 and October 2022, at King Chulalongkorn Memorial Hospital, Thailand.
Article Synopsis
- The study explored how fibrin-based hydrogel encapsulation impacted ovarian tissue quality and cell survival after freezing
- The research involved four groups of ovarian cortex from patients aged 44-47, comparing fresh tissue, uncapsulated tissue, and two encapsulated conditions (with and without VEGF)
- Key findings showed that encapsulation helped maintain signaling pathways important for cell survival, although some unfavorable effects on certain expression levels were noted, suggesting a need for further research to enhance this technique for fertility preservation.
This study aimed to compare the efficacies of conventional and non-conventional (modified hydrostatic microfluidic pumpless device, MHPD) systems on ovarian tissue culture and in vitro follicle growth using a mouse model. A total of 56 ovarian cortical tissues retrieved from seven wild-type mice were divided into three groups: 1) fresh control, 2) conventional culture system (control), and 3) non-conventional system with MHPD. Ovarian tissues were cultured for 96 hours and evaluated for follicle morphology, developmental stage, intact follicle density, and relative gene expression levels (proliferating cell nuclear antigen, insulin like growth factor 1, BAX, and Bcl-2).
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- The study explored how encapsulating ovarian tissue from domestic cats in a biodegradable hydrogel affects its ability to survive cryopreservation and maintain follicle quality during culture.
- The experiments involved comparing ovarian tissues with hydrogel encapsulation to controls, assessing factors like follicle viability and morphology after slow freezing and vitrification.
- Results showed that hydrogel encapsulation improved follicle survival and quality, suggesting potential methods for enhancing human fertility preservation through better cryopreservation techniques.
Objective: The long interspersed elements (LINE-1, L1s) are a group of genetic elements found in large numbers in the human genome that can translate into phenotype by controlling genes. Growing evidence supports the role of epigenetic in polycystic ovary syndrome (PCOS). The purpose of this study is to evaluate the DNA methylation levels in LINE-1 in a tissue-specific manner using cumulus cells from patients with PCOS compared with normal controls.
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