Protein overabundance is driven by growth robustness.

Protein expression levels optimize cell fitness: Too low an expression level of essential proteins will slow growth by compromising essential processes; whereas overexpression slows growth by increasing the metabolic load. This trade-off naïvely predicts that cells maximize their fitness by sufficiency, expressing just enough of each essential protein for function. We test this prediction in the naturally-competent bacterium by characterizing the proliferation dynamics of essential-gene knockouts at a single-cell scale (by imaging) as well as at a genome-wide scale (by TFNseq).

A cytochrome repressed by a MarR family regulator confers resistance to metals, nitric oxide, sulfide, and cyanide in .

Unlabelled: is a ubiquitous environmental pathogen. Despite its remarkable adaptability, little is known about the mechanisms of stress resistance in this bacterium. Here, in a screen for iron-susceptible transposon mutants, we identified a cytochrome that protects against multiple stresses.

Protein overabundance is driven by growth robustness.

Protein expression levels optimize cell fitness: Too low an expression level of essential proteins will slow growth by compromising essential processes; whereas overexpression slows growth by increasing the metabolic load. This trade-off naïvely predicts that cells maximize their fitness by sufficiency, expressing just enough of each essential protein for function. We test this prediction in the naturally-competent bacterium by characterizing the proliferation dynamics of essential-gene knockouts at a single-cell scale (by imaging) as well as at a genome-wide scale (by TFNseq).

The Effect of Neuropriming and Focus of Attention on Amateur Standing Long Jump Performance.

Non-invasive brain stimulation has been prominent in recent neurophysiology research. The use of brain stimulation has not been examined in combination with the focus of attention paradigm, an established motor control tool. Therefore, the purpose of this study was to examine the effects of both brain stimulation and focus of attention on the outcome performance, peak force, lower extremity joint kinematics, and projection angle of a standing long jump.

Nursing students' reflections on vaccine administration during the COVID-19 global pandemic.

Background: During the COVID-19 pandemic, many nursing schools limited in-person clinical instruction to lower the risk of student exposure to SARS-CoV-2. Some U.S.