Smoking impairs alveolar macrophage activation after inert dust exposure.

Magnetopneumography was applied to investigate intracellular phagosome motion in alveolar macrophage cells of healthy subjects (non-smokers and smokers). Ingested magnetic microparticles are inhaled and phagocytized by alveolar macrophages within hours. Thereby the particles are transferred into phagolysosomes.

Tumor response to ionizing radiation and combined 2-deoxy-D-glucose application in EATC tumor bearing mice: monitoring of tumor size and microscopic observations.

The present study deals with the changes induced by two fractionation schedules (5 x 9 Gy and 10 x 4.5 Gy; 30 MeV-electrons) of ionizing radiations and 2-deoxy-D-glucose (2-DG) application on EATC tumor bearing swiss albino mice. The monitoring of tumor response was carried out by means of caliper measurement on the macroscopic level and by histopathological examination of tumor preparations stained with hematoxiline and eosine on the microscopic level.

The RBE of negative pions in the treatment of tumors with different volumes.

For negative pions, large differences exist between experimental RBE values obtained by in vivo investigations with animals and corresponding data being used in clinical tumor therapy. Therefore, the influence of the treatment volume on the radiation quality and respectively on the RBE is examined. The RBE for euoxic mammalian cells is measured with high precision in different volumes irradiated in the spot-scan mode with the PIOTRON at the Paul Scherrer Institute.

Reversibility of inhibition of DNA double strand break repair by 2-deoxy-D-glucose in Ehrlich ascites tumour cells.

The glycolytic inhibitor 2-deoxy-D-glucose (2-DG), a potent therapeutic adjuvant in cancer radiotherapy, was tested for the reversibility of its inhibitory action on X-ray-induced DNA double strand break (dsb) repair. Cells were exposed to 40 Gy of X-rays and allowed to repair with or without 2-DG in suspension at 37 degrees C. DNA dsb rejoining was measured by pulsed field gel electrophoresis.

Effect of 2-deoxy-D-glucose on DNA double strand break repair, cell survival and energy metabolism in euoxic Ehrlich ascites tumour cells.

Effects of 2-deoxy-D-glucose (2-DG) on DNA double strand break (dsb) repair, cell survival and on the energy metabolism were investigated in exponentially growing Ehrlich ascites tumour (EAT) cells. Cells in suspension were exposed to 40 Gy of X-rays and allowed to repair (up to 4 h) with or without 2-DG at 37 degrees C. DNA dsb rejoining was measured by means of clamped homogeneous electric field (CHEF), a pulsed field gel electrophoresis technique.